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GeneTex
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Biorbyt
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Image Search Results
Journal: Cell Death and Differentiation
Article Title: Enhancer of polycomb maintains germline activity and genome integrity in Drosophila testis
doi: 10.1038/s41418-017-0056-5
Figure Lengend Snippet: E(Pc) germline clones exhibit increased γH2Av. a–l Apical tip of testes stained with GFP b, f, j to identify GFP-negative E(Pc) mutant clones, DAPI c, g, k, γH2Av d, h and H2Av l. m Quantification of intensity of γH2Av and H2Av signals between GFP-negative E(Pc) mutant clones and GFP-positive control germ cells. γH2Avclone/γH2Avctrl = 4.05 ± 2.58. H2Avclone/H2Avctrl = 1.00 ± 0.33 (mean ± SD). Two-tailed t-test. ****P < 0.0001. n–p Apical tip of hs-I-CreI testes before heat shock (no HS) n, a half day after heat shock (0.5D AHS) o and 3 days after heat shock (3D AHS) p stained with hub and cyst cell marker Arm and DNA DSB marker γH2Av. q–s Apical tip of hs-I-CreI, tub-Gal80ts, nos > E(Pc) shRNA testes before heat shock q, 0.5D AHS r, and 3D AHS s stained with Arm and γH2Av. Asterisk: hub. Scale bar: 20 μm
Article Snippet: Primary antibodies were as follows: Vasa (rabbit, 1:200, Santa Cruz, sc-30,210), Vasa (rat, 1:50, DSHB), Fas III (mouse, 1:100, DSHB, 7G10), Armadillo (mouse, 1:200, DSHB, N2 7A1), α-spectrin (mouse, 1:50, DSHB, 3A9), GFP (chicken, 1:1000, Abcam, ab13970), STAT92E (rabbit, 1:800, from Denise Montell, UC Santa Barbara), pMAD (rabbit, 1:100, Abcam, ab52903), DE-Cad (rat, 1:20, DSHB, DCAD2), anti-Ser10-phosphorylated Histone H3 (mouse, 1:1000, Millipore, #05–806), H3K27me3 (rabbit, 1:2000, Millipore, #07–449), H3K4me3 (rabbit, 1:2000, Millipore, #07–473), H2Av (rabbit, 1:500, active motif, 39,716),
Techniques: Clone Assay, Staining, Mutagenesis, Positive Control, Two Tailed Test, Marker, shRNA
Journal: Cell
Article Title: Recycling of modified H2A-H2B provides short-term memory of chromatin states
doi: 10.1016/j.cell.2023.01.007
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Purification, Western Blot, Software