h2a ub Search Results


ha-h2a kr2 -ub (h2a-k13,15,118,119,125,127,129r-ub-kless)  (GenScript corporation)
90
GenScript corporation ha-h2a kr2 -ub (h2a-k13,15,118,119,125,127,129r-ub-kless)
USP48 antagonizes BRCA1-mediated resection. a Increased resection seen on USP48 knockdown requires BRCA1 and SMARCAD1. Resection lengths were measured in HeLa cells depleted as indicated. Cells were treated with 10 mM BrdU for 24 h with addition of 10 µM Olaparib for the final 16 h. Cells were lysed and DNA fibres spread before staining for BrdU-positive single-stranded DNA resection tracks. n = 190 tracks for each condition. Bars indicate median, also shown numerically in brackets. *** p < 0.005 Mann–Whitney test. b Western blottings to demonstrate protein expression levels in HeLa cells following siRNA as indicated. c Rad51 foci formation in S-phase (EdU positive) HeLa cells depleted as indicated. Cells were fixed at 2 h post 5 Gy IR. Scale bars = 10 µm (see Supplementary Fig. for quantification). d Suppressive impact of USP48 Iso2 overexpression on resection lengths can be rescued by co-expression of an uncleavable <t>H2A-Ub</t> fusion. Resection lengths were measured in untransfected HeLa cells or those transfected with USP48 Iso2 and expressing either H2A or H2A-Ub fusion <t>(KR2</t> denotes that lysines 13/15, 118/119, and 125/127/129 were mutated to arginines). Cells were prepared as in a and 210 tracks were measured for each condition. Bars indicate median, also shown numerically in brackets. *** p < 0.005, NS = nonsignificant, Mann–Whitney test. e USP48 restricts positioning of 53BP1 in damage foci. Images of BRCA1 and 53BP1 in cells treated with control or USP48 siRNA exposed to 2 Gy IR and fixed 8 h later. Scale bars = 2 µm. Quantification of mean relative intensity profiles for co-localizing foci. n = 25, bars = SEM. Right panel shows western blotting of USP48 protein levels
Ha H2a Kr2 Ub (H2a K13,15,118,119,125,127,129r Ub Kless), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ha-h2a kr2 -ub (h2a-k13,15,118,119,125,127,129r-ub-kless) - by Bioz Stars, 2026-04
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USP48 antagonizes BRCA1-mediated resection. a Increased resection seen on USP48 knockdown requires BRCA1 and SMARCAD1. Resection lengths were measured in HeLa cells depleted as indicated. Cells were treated with 10 mM BrdU for 24 h with addition of 10 µM Olaparib for the final 16 h. Cells were lysed and DNA fibres spread before staining for BrdU-positive single-stranded DNA resection tracks. n = 190 tracks for each condition. Bars indicate median, also shown numerically in brackets. *** p < 0.005 Mann–Whitney test. b Western blottings to demonstrate protein expression levels in HeLa cells following siRNA as indicated. c Rad51 foci formation in S-phase (EdU positive) HeLa cells depleted as indicated. Cells were fixed at 2 h post 5 Gy IR. Scale bars = 10 µm (see Supplementary Fig. for quantification). d Suppressive impact of USP48 Iso2 overexpression on resection lengths can be rescued by co-expression of an uncleavable H2A-Ub fusion. Resection lengths were measured in untransfected HeLa cells or those transfected with USP48 Iso2 and expressing either H2A or H2A-Ub fusion (KR2 denotes that lysines 13/15, 118/119, and 125/127/129 were mutated to arginines). Cells were prepared as in a and 210 tracks were measured for each condition. Bars indicate median, also shown numerically in brackets. *** p < 0.005, NS = nonsignificant, Mann–Whitney test. e USP48 restricts positioning of 53BP1 in damage foci. Images of BRCA1 and 53BP1 in cells treated with control or USP48 siRNA exposed to 2 Gy IR and fixed 8 h later. Scale bars = 2 µm. Quantification of mean relative intensity profiles for co-localizing foci. n = 25, bars = SEM. Right panel shows western blotting of USP48 protein levels

Journal: Nature Communications

Article Title: USP48 restrains resection by site-specific cleavage of the BRCA1 ubiquitin mark from H2A

doi: 10.1038/s41467-017-02653-3

Figure Lengend Snippet: USP48 antagonizes BRCA1-mediated resection. a Increased resection seen on USP48 knockdown requires BRCA1 and SMARCAD1. Resection lengths were measured in HeLa cells depleted as indicated. Cells were treated with 10 mM BrdU for 24 h with addition of 10 µM Olaparib for the final 16 h. Cells were lysed and DNA fibres spread before staining for BrdU-positive single-stranded DNA resection tracks. n = 190 tracks for each condition. Bars indicate median, also shown numerically in brackets. *** p < 0.005 Mann–Whitney test. b Western blottings to demonstrate protein expression levels in HeLa cells following siRNA as indicated. c Rad51 foci formation in S-phase (EdU positive) HeLa cells depleted as indicated. Cells were fixed at 2 h post 5 Gy IR. Scale bars = 10 µm (see Supplementary Fig. for quantification). d Suppressive impact of USP48 Iso2 overexpression on resection lengths can be rescued by co-expression of an uncleavable H2A-Ub fusion. Resection lengths were measured in untransfected HeLa cells or those transfected with USP48 Iso2 and expressing either H2A or H2A-Ub fusion (KR2 denotes that lysines 13/15, 118/119, and 125/127/129 were mutated to arginines). Cells were prepared as in a and 210 tracks were measured for each condition. Bars indicate median, also shown numerically in brackets. *** p < 0.005, NS = nonsignificant, Mann–Whitney test. e USP48 restricts positioning of 53BP1 in damage foci. Images of BRCA1 and 53BP1 in cells treated with control or USP48 siRNA exposed to 2 Gy IR and fixed 8 h later. Scale bars = 2 µm. Quantification of mean relative intensity profiles for co-localizing foci. n = 25, bars = SEM. Right panel shows western blotting of USP48 protein levels

Article Snippet: HA-H2A and HA-H2A KR2 -Ub (H2A-K13,15,118,119,125,127,129R-Ub-Kless) were described previously and all H2A constructs including HA-H2A KR1 -Ub (H2A-125,127,129R-Ub-Kless) were originally synthesized by Genscript.

Techniques: Knockdown, Staining, MANN-WHITNEY, Western Blot, Expressing, Over Expression, Transfection, Control