g2565ba fluorescent scanner Search Results


93
Agilent technologies situ hybridization kit plus
Situ Hybridization Kit Plus, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Crna Cy3 Fluorescence Labeling, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Agilent technologies dna microarray scanner g2565ba
Dna Microarray Scanner G2565ba, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Agilent technologies microarray scanner
Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a <t>microarray</t> scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).
Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Agilent technologies dna microarray scanner
Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a <t>microarray</t> scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).
Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Agilent technologies laser microarray scanner g2565ba fluorescent scanner
Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a <t>microarray</t> scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).
Laser Microarray Scanner G2565ba Fluorescent Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Agilent technologies laser confocal scanner
Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a <t>microarray</t> scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).
Laser Confocal Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Agilent technologies hybridization
Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a <t>microarray</t> scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).
Hybridization, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Agilent technologies genechip
Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a <t>microarray</t> scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).
Genechip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Agilent technologies dearray software
Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a <t>microarray</t> scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).
Dearray Software, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Agilent technologies fluorescent cyanine 3-ctp–labeled crna
Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a <t>microarray</t> scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).
Fluorescent Cyanine 3 Ctp–Labeled Crna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Agilent technologies feature extraction software version 11
Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a <t>microarray</t> scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).
Feature Extraction Software Version 11, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a microarray scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).

Journal: BMC Pharmacology & Toxicology

Article Title: 3-D DNA methylation phenotypes correlate with cytotoxicity levels in prostate and liver cancer cell models

doi: 10.1186/2050-6511-14-11

Figure Lengend Snippet: Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a microarray scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).

Article Snippet: Fluorescence detection was performed comparatively at 5 microns resolution with a microarray scanner (G2565BA, Agilent Technologies) equipped with a helium-neon laser (633 nm) to excite Cy3, and the above-mentioned confocal microscope with a Plan-Apo 10X 0.7 lens.

Techniques: Immunofluorescence, Concentration Assay, Methylation, Microarray, Binding Assay, Microscopy