frameshift mutation Search Results


frameshift mutations  (Kunkel GmbH)
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Frameshift Mutations, supplied by Kunkel GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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frameshift mutation p.pro1719arg*6  (Trudeau Institute Inc)
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Trudeau Institute Inc frameshift mutation p.pro1719arg*6
Frameshift Mutation P.Pro1719arg*6, supplied by Trudeau Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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homopolymer track frameshift mutation 482_483insc  (Illumina Inc)
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Illumina Inc homopolymer track frameshift mutation 482_483insc
Homopolymer Track Frameshift Mutation 482 483insc, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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frameshift mutations  (Blackwell Science Ltd)
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Blackwell Science Ltd frameshift mutations
Frameshift Mutations, supplied by Blackwell Science Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mutation surveyor for frameshift mutations  (SoftGenetics)
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SoftGenetics mutation surveyor for frameshift mutations
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frameshift mutation c.222_223del  (Shinagawa Corporation)
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Shinagawa Corporation frameshift mutation c.222_223del
Frameshift Mutation C.222 223del, supplied by Shinagawa Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene mutation total hd mit mtbd aaa total frameshift  (Pathogenica Inc)
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Gene Mutation Total Hd Mit Mtbd Aaa Total Frameshift, supplied by Pathogenica Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hifi-validated frameshift mutations  (Illumina Inc)
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Illumina Inc hifi-validated frameshift mutations
Hifi Validated Frameshift Mutations, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wsmv nia-pro and cp cistrons with frameshift and nucleotide substitution mutations  (GenScript corporation)
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GenScript corporation wsmv nia-pro and cp cistrons with frameshift and nucleotide substitution mutations
Wsmv Nia Pro And Cp Cistrons With Frameshift And Nucleotide Substitution Mutations, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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frameshift mutation in the nrip1 coding sequence (rip msi)  (Illumina Inc)
90
Illumina Inc frameshift mutation in the nrip1 coding sequence (rip msi)
Biological characterization of the RIP MSI <t>frameshift</t> mutation. ( A ) Intrinsic transrepression assay in HCT116LR cells transiently transfected with increasing doses of the Gal4DBD-fused NRIP1 and RIP MSI expression vectors. ( B ) MSH2 and MSH6 mRNA levels in RIPKO MEFs stably expressing GFP, GFP-RIP140 or GFP-RIP MSI ; n = 3 independent experiments. ( C ) Luciferase assay with the MSH2 gene reporter and increasing doses of NRIP1 or RIP MSI expression vectors (mean ± SD; n = 3 independent experiments). ( D ) MSH2 and MSH6 mRNA levels in HT29 cells transiently transfected with pEGFP, pEGFP-RIP140 or pEGFP-RIP MSI expression vectors. ( E ) MSH2 and MSH6 mRNA levels in stably transfected MEF-WT MSI and MEF-KO MSI . Data were normalized as compared to WT and RIPKO control cells expressing GFP alone and set at 1. ( F ) Cell proliferation of RIPKO MEFs stably expressing GFP, GFP-RIP140 or GFP-RIP MSI at day 4 and 6 after seeding. Results are the fold-change vs. control (GFP) cells after normalization to the cell density at day 1; n = 3 independent experiments. ( G ) Cell proliferation of human RKO CRC cells transiently transfected with pEGFP, pEGFP-RIP140 or pEGFP-RIP MSI expression vectors over 8 days. Results are the fold-change (triplicates) after normalization to the cell density at day 0. For all panels: data are the mean ± SD; ns = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 (Kruskal–Wallis test).
Frameshift Mutation In The Nrip1 Coding Sequence (Rip Msi), supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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frameshift mutation c.376_378delinstt, p.leu126serfsx6  (SAS institute)
90
SAS institute frameshift mutation c.376_378delinstt, p.leu126serfsx6
Biological characterization of the RIP MSI <t>frameshift</t> mutation. ( A ) Intrinsic transrepression assay in HCT116LR cells transiently transfected with increasing doses of the Gal4DBD-fused NRIP1 and RIP MSI expression vectors. ( B ) MSH2 and MSH6 mRNA levels in RIPKO MEFs stably expressing GFP, GFP-RIP140 or GFP-RIP MSI ; n = 3 independent experiments. ( C ) Luciferase assay with the MSH2 gene reporter and increasing doses of NRIP1 or RIP MSI expression vectors (mean ± SD; n = 3 independent experiments). ( D ) MSH2 and MSH6 mRNA levels in HT29 cells transiently transfected with pEGFP, pEGFP-RIP140 or pEGFP-RIP MSI expression vectors. ( E ) MSH2 and MSH6 mRNA levels in stably transfected MEF-WT MSI and MEF-KO MSI . Data were normalized as compared to WT and RIPKO control cells expressing GFP alone and set at 1. ( F ) Cell proliferation of RIPKO MEFs stably expressing GFP, GFP-RIP140 or GFP-RIP MSI at day 4 and 6 after seeding. Results are the fold-change vs. control (GFP) cells after normalization to the cell density at day 1; n = 3 independent experiments. ( G ) Cell proliferation of human RKO CRC cells transiently transfected with pEGFP, pEGFP-RIP140 or pEGFP-RIP MSI expression vectors over 8 days. Results are the fold-change (triplicates) after normalization to the cell density at day 0. For all panels: data are the mean ± SD; ns = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 (Kruskal–Wallis test).
Frameshift Mutation C.376 378delinstt, P.Leu126serfsx6, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mbd4/ med1 gene  (Morishita Jintan)
90
Morishita Jintan mbd4/ med1 gene
Biological characterization of the RIP MSI <t>frameshift</t> mutation. ( A ) Intrinsic transrepression assay in HCT116LR cells transiently transfected with increasing doses of the Gal4DBD-fused NRIP1 and RIP MSI expression vectors. ( B ) MSH2 and MSH6 mRNA levels in RIPKO MEFs stably expressing GFP, GFP-RIP140 or GFP-RIP MSI ; n = 3 independent experiments. ( C ) Luciferase assay with the MSH2 gene reporter and increasing doses of NRIP1 or RIP MSI expression vectors (mean ± SD; n = 3 independent experiments). ( D ) MSH2 and MSH6 mRNA levels in HT29 cells transiently transfected with pEGFP, pEGFP-RIP140 or pEGFP-RIP MSI expression vectors. ( E ) MSH2 and MSH6 mRNA levels in stably transfected MEF-WT MSI and MEF-KO MSI . Data were normalized as compared to WT and RIPKO control cells expressing GFP alone and set at 1. ( F ) Cell proliferation of RIPKO MEFs stably expressing GFP, GFP-RIP140 or GFP-RIP MSI at day 4 and 6 after seeding. Results are the fold-change vs. control (GFP) cells after normalization to the cell density at day 1; n = 3 independent experiments. ( G ) Cell proliferation of human RKO CRC cells transiently transfected with pEGFP, pEGFP-RIP140 or pEGFP-RIP MSI expression vectors over 8 days. Results are the fold-change (triplicates) after normalization to the cell density at day 0. For all panels: data are the mean ± SD; ns = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 (Kruskal–Wallis test).
Mbd4/ Med1 Gene, supplied by Morishita Jintan, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Biological characterization of the RIP MSI frameshift mutation. ( A ) Intrinsic transrepression assay in HCT116LR cells transiently transfected with increasing doses of the Gal4DBD-fused NRIP1 and RIP MSI expression vectors. ( B ) MSH2 and MSH6 mRNA levels in RIPKO MEFs stably expressing GFP, GFP-RIP140 or GFP-RIP MSI ; n = 3 independent experiments. ( C ) Luciferase assay with the MSH2 gene reporter and increasing doses of NRIP1 or RIP MSI expression vectors (mean ± SD; n = 3 independent experiments). ( D ) MSH2 and MSH6 mRNA levels in HT29 cells transiently transfected with pEGFP, pEGFP-RIP140 or pEGFP-RIP MSI expression vectors. ( E ) MSH2 and MSH6 mRNA levels in stably transfected MEF-WT MSI and MEF-KO MSI . Data were normalized as compared to WT and RIPKO control cells expressing GFP alone and set at 1. ( F ) Cell proliferation of RIPKO MEFs stably expressing GFP, GFP-RIP140 or GFP-RIP MSI at day 4 and 6 after seeding. Results are the fold-change vs. control (GFP) cells after normalization to the cell density at day 1; n = 3 independent experiments. ( G ) Cell proliferation of human RKO CRC cells transiently transfected with pEGFP, pEGFP-RIP140 or pEGFP-RIP MSI expression vectors over 8 days. Results are the fold-change (triplicates) after normalization to the cell density at day 0. For all panels: data are the mean ± SD; ns = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 (Kruskal–Wallis test).

Journal: Cancers

Article Title: A Truncated NRIP1 Mutant Amplifies Microsatellite Instability of Colorectal Cancer by Regulating MSH2/MSH6 Expression, and Is a Prognostic Marker of Stage III Tumors

doi: 10.3390/cancers13174449

Figure Lengend Snippet: Biological characterization of the RIP MSI frameshift mutation. ( A ) Intrinsic transrepression assay in HCT116LR cells transiently transfected with increasing doses of the Gal4DBD-fused NRIP1 and RIP MSI expression vectors. ( B ) MSH2 and MSH6 mRNA levels in RIPKO MEFs stably expressing GFP, GFP-RIP140 or GFP-RIP MSI ; n = 3 independent experiments. ( C ) Luciferase assay with the MSH2 gene reporter and increasing doses of NRIP1 or RIP MSI expression vectors (mean ± SD; n = 3 independent experiments). ( D ) MSH2 and MSH6 mRNA levels in HT29 cells transiently transfected with pEGFP, pEGFP-RIP140 or pEGFP-RIP MSI expression vectors. ( E ) MSH2 and MSH6 mRNA levels in stably transfected MEF-WT MSI and MEF-KO MSI . Data were normalized as compared to WT and RIPKO control cells expressing GFP alone and set at 1. ( F ) Cell proliferation of RIPKO MEFs stably expressing GFP, GFP-RIP140 or GFP-RIP MSI at day 4 and 6 after seeding. Results are the fold-change vs. control (GFP) cells after normalization to the cell density at day 1; n = 3 independent experiments. ( G ) Cell proliferation of human RKO CRC cells transiently transfected with pEGFP, pEGFP-RIP140 or pEGFP-RIP MSI expression vectors over 8 days. Results are the fold-change (triplicates) after normalization to the cell density at day 0. For all panels: data are the mean ± SD; ns = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001 (Kruskal–Wallis test).

Article Snippet: For samples from ICM and CRCT, the frameshift mutation in the NRIP1 coding sequence (RIP MSI ) was detected by Illumina HiSeq4000 deep-sequencing (Integragen genomics, France) using specific primers ( ) and DNA extracted using the QIAamp DNA FFPE kit (Qiagen, Hilden, Germany).

Techniques: Mutagenesis, Transfection, Expressing, Stable Transfection, Luciferase, Control

RIP MSI frameshift mutation and survival of patients with CRC. Kaplan–Meier plots of OS rate in patients with CRC divided according to the presence/absence of the RIP MSI mutation in the tumor DNA. ( A ) Whole cohort ( n = 189); Patients with ( B ) stage I/II CRC ( n = 127); ( C ) stage III CRC ( n = 62); ( D ) stage III CRC with dMLH1 ( n = 40); ( E ) stage III CRC and proficient MLH1 ( n = 20). ( F ) Forest plot of the associations between the indicated variables and OS (Cox univariate analysis; * p < 0.05 and ** p < 0.01) in the whole cohort and in the indicated subgroups. HR, Cox Hazard Ratio; CI, Confidence Interval of HR.

Journal: Cancers

Article Title: A Truncated NRIP1 Mutant Amplifies Microsatellite Instability of Colorectal Cancer by Regulating MSH2/MSH6 Expression, and Is a Prognostic Marker of Stage III Tumors

doi: 10.3390/cancers13174449

Figure Lengend Snippet: RIP MSI frameshift mutation and survival of patients with CRC. Kaplan–Meier plots of OS rate in patients with CRC divided according to the presence/absence of the RIP MSI mutation in the tumor DNA. ( A ) Whole cohort ( n = 189); Patients with ( B ) stage I/II CRC ( n = 127); ( C ) stage III CRC ( n = 62); ( D ) stage III CRC with dMLH1 ( n = 40); ( E ) stage III CRC and proficient MLH1 ( n = 20). ( F ) Forest plot of the associations between the indicated variables and OS (Cox univariate analysis; * p < 0.05 and ** p < 0.01) in the whole cohort and in the indicated subgroups. HR, Cox Hazard Ratio; CI, Confidence Interval of HR.

Article Snippet: For samples from ICM and CRCT, the frameshift mutation in the NRIP1 coding sequence (RIP MSI ) was detected by Illumina HiSeq4000 deep-sequencing (Integragen genomics, France) using specific primers ( ) and DNA extracted using the QIAamp DNA FFPE kit (Qiagen, Hilden, Germany).

Techniques: Mutagenesis