Journal: Journal of cellular and molecular medicine

Article Title: Orderly Regulation of Macrophages and Fibroblasts by Axl in Bleomycin-Induced Pulmonary Fibrosis in Mice.

doi: 10.1111/jcmm.70321

Figure Lengend Snippet: FIGURE 8 | Axl deficiency could inhibit the proliferation and migration of fibroblasts. The following experiments were performed after prima- ry fibroblasts were treated with TGF-β or PBS for 48 h. (A) EDU incubation for 2 h and immunofluorescence detection. Scale bars: All 20 μm. (B) Quantitative results in Figure A. (C) The results of cell proliferation were detected by CCK-8. (D) Photograph of the cell invasion experiment de- scribed above. Scale bars: All 100 μm. (E) The invasion experiment of the above cells was quantitative, and five random visual fields were selected from each Transwell under ×400 magnification for statistics. (F) Cell proteins were extracted and the protein levels of collagen Type I were assessed by western blotting. (G) Quantitative results in Figure F. (H) The cells were stained by immunofluorescence with PXN, phalloidin and Axl, and then photographed under confocal microscopy. Scale bars: All 20 μm. (I) PXN was quantified in Figure H. A total of 15 cells were selected from each slide under ×400 magnification for quantification. n = 3 mice/group, */***p < 0.05/0.001.

Article Snippet: After primary fibroblast serum starvation for 12–24 h, the medium was changed and 5 ng/mL TGF- β (MedChem Express, nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jcm m .70321 by IN A SP - N E PA L , W iley O nline L ibrary on [11/01/2025].

Techniques: Migration, Incubation, Immunofluorescence, CCK-8 Assay, Western Blot, Staining, Confocal Microscopy

Journal: Journal of cellular and molecular medicine

Article Title: Orderly Regulation of Macrophages and Fibroblasts by Axl in Bleomycin-Induced Pulmonary Fibrosis in Mice.

doi: 10.1111/jcmm.70321

Figure Lengend Snippet: FIGURE 9 | Inhibition of Axl or FAK can reduce the activation of PXN in fibroblasts. The extracted primary fibroblasts were treated with inhib- itors for 4 h, then TGF-β or PBS were added and follow-up experiments were performed 48 h later. The concentration of R428 was 2 mmol/L, and the concentration of PF-573228 was 3 mmol/L. (A) Photograph of the cell invasion experiment described above (original magnification: ×200). (B) The invasion experiment of the above cells was quantitative, and five random visual fields were selected from each Transwell under ×400 magnification for statistics. (C) Cell proteins were extracted and the protein levels of β-tubulin, PXN, p-PXN, FAK and p-FAK were assessed by western blotting. (D) Protein quantification of p-FAK in Figure C. (E) Protein quantification of p-paxillin in Figure C. n = 3 mice/group, */***p < 0.05/0.001.

Article Snippet: After primary fibroblast serum starvation for 12–24 h, the medium was changed and 5 ng/mL TGF- β (MedChem Express, nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jcm m .70321 by IN A SP - N E PA L , W iley O nline L ibrary on [11/01/2025].

Techniques: Inhibition, Activation Assay, Concentration Assay, Western Blot