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ecotropic packaging plasmid pik6.1mcv.ecopac.utd  (GeNOsys Inc)
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GeNOsys Inc ecotropic packaging plasmid pik6.1mcv.ecopac.utd
(A) The structure of retroviral cDNA construct. The cDNAs of proto-oncogene and homeobox gene families were obtained from Mammalian Gene Collection (MGC). Individual cDNA was amplified by PCR with the corresponding primers synthesized for each cDNA to include an HA tag in the 5’ position. The 5’ and 3’ UTRs were removed by amplifying the coding sequences only. To produce the retroviral mixture, we mixed a constant amount (0.1 μg for each cDNA) of retroviral plasmid for every cDNA, and the cDNA mixture was transfected into the 293T cells along with <t>ecotropic</t> packaging plasmid <t>pIK6.1MCV.ecopac.UTD</t> (Ecopac: M. Finer Cell Genosys, Redwood City, CA). (B) We generally achieved 10 to 30% infection rate using the fresh mouse bone marrow cells as the target. Four μg/ml polybrene was included in the transfection medium along with the cytokine mixture described in Materials and Method. (C) Survival kinetics of mice transplanted with bone marrow cells transduced with the retroviral cDNA library. One million retrovirus-transduced cells were transplanted into syngenic γ-irradiated host mice through tail vain. Ten host mice were transplanted with the virus-transduced cells, and the mice showing the leukemic symptoms were sacrificed and the leukemic cells were analyzed in terms of surface phenotype and cDNA insert(s). The control mice (n = 10) were transplanted with bone marrow cells transduced with MSCV.IRES.GFP. No mice in the control group showed any sign of illness during the observation period (200 days).
Ecotropic Packaging Plasmid Pik6.1mcv.Ecopac.Utd, supplied by GeNOsys Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecotropic+packaging+plasmids/ecotropic+packaging+plasmid+pik6+1mcv+ecopac+utd/pmc04659616-36-12-20
Average 90 stars, based on 1 article reviews
ecotropic packaging plasmid pik6.1mcv.ecopac.utd - by Bioz Stars, 2026-09
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(A) The structure of retroviral cDNA construct. The cDNAs of proto-oncogene and homeobox gene families were obtained from Mammalian Gene Collection (MGC). Individual cDNA was amplified by PCR with the corresponding primers synthesized for each cDNA to include an HA tag in the 5’ position. The 5’ and 3’ UTRs were removed by amplifying the coding sequences only. To produce the retroviral mixture, we mixed a constant amount (0.1 μg for each cDNA) of retroviral plasmid for every cDNA, and the cDNA mixture was transfected into the 293T cells along with ecotropic packaging plasmid pIK6.1MCV.ecopac.UTD (Ecopac: M. Finer Cell Genosys, Redwood City, CA). (B) We generally achieved 10 to 30% infection rate using the fresh mouse bone marrow cells as the target. Four μg/ml polybrene was included in the transfection medium along with the cytokine mixture described in Materials and Method. (C) Survival kinetics of mice transplanted with bone marrow cells transduced with the retroviral cDNA library. One million retrovirus-transduced cells were transplanted into syngenic γ-irradiated host mice through tail vain. Ten host mice were transplanted with the virus-transduced cells, and the mice showing the leukemic symptoms were sacrificed and the leukemic cells were analyzed in terms of surface phenotype and cDNA insert(s). The control mice (n = 10) were transplanted with bone marrow cells transduced with MSCV.IRES.GFP. No mice in the control group showed any sign of illness during the observation period (200 days).

Journal: PLoS ONE

Article Title: Characterization of Leukemia-Inducing Genes Using a Proto-Oncogene/Homeobox Gene Retroviral Human cDNA Library in a Mouse In Vivo Model

doi: 10.1371/journal.pone.0143240

Figure Lengend Snippet: (A) The structure of retroviral cDNA construct. The cDNAs of proto-oncogene and homeobox gene families were obtained from Mammalian Gene Collection (MGC). Individual cDNA was amplified by PCR with the corresponding primers synthesized for each cDNA to include an HA tag in the 5’ position. The 5’ and 3’ UTRs were removed by amplifying the coding sequences only. To produce the retroviral mixture, we mixed a constant amount (0.1 μg for each cDNA) of retroviral plasmid for every cDNA, and the cDNA mixture was transfected into the 293T cells along with ecotropic packaging plasmid pIK6.1MCV.ecopac.UTD (Ecopac: M. Finer Cell Genosys, Redwood City, CA). (B) We generally achieved 10 to 30% infection rate using the fresh mouse bone marrow cells as the target. Four μg/ml polybrene was included in the transfection medium along with the cytokine mixture described in Materials and Method. (C) Survival kinetics of mice transplanted with bone marrow cells transduced with the retroviral cDNA library. One million retrovirus-transduced cells were transplanted into syngenic γ-irradiated host mice through tail vain. Ten host mice were transplanted with the virus-transduced cells, and the mice showing the leukemic symptoms were sacrificed and the leukemic cells were analyzed in terms of surface phenotype and cDNA insert(s). The control mice (n = 10) were transplanted with bone marrow cells transduced with MSCV.IRES.GFP. No mice in the control group showed any sign of illness during the observation period (200 days).

Article Snippet: The retroviral vector plasmids were transfected into the 293T cells along with ecotropic packaging plasmid pIK6.1MCV.ecopac.UTD (Ecopac: M. Finer Cell Genosys, Redwood City, CA).

Techniques: Construct, Amplification, Synthesized, Plasmid Preparation, Transfection, Infection, Transduction, cDNA Library Assay, Irradiation