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Image Search Results
Journal: Communications Biology
Article Title: Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production
doi: 10.1038/s42003-020-0910-8
Figure Lengend Snippet: a Schematic illustration of FFA production pathway. The truncated acyl-CoA thioesterase gene (‘ tesA ), under control of P clac143 inducible promoter, was inserted into the chromosome with simultaneous knockout of the endogenous long-chain-fatty-acid--CoA ligase ( fadD ). ‘TesA converts fatty acyl-ACP to free fatty acid and its reconversion to fatty acyl-CoA is negated by knocking out the endogenous fadD . Comparison of the growth and FFA productivity of the engineered Synechococcus sp. PCC 11901 and PCC 7002 strains using regular AD7 ( b ) and MAD ( c ) medium. Filled and dashed lines indicate growth (OD 730 measurements) of the induced and non-induced cultures respectively, while the column bars correspond to the FFA production. Arrows indicate addition of IPTG inducer and light intensity increase. d Comparison of growth and FFA production by the WT (dashed lines and columns) and Δ fadD knockout control (filled lines and columns) strains. e Analysis of FFA production by the engineered PCC 11901 strain induced at OD 730 ≈ 8. FFA were extracted either directly from the culture or from the medium supernatant. For OD 730 measurements data points with error bars represent mean of n = 3 biological replicates ±standard deviation (for PCC 11901 Δ fadD :: tesA strain n = 2 in panels b and c and n = 3 in panel e ). FFA concentration as mean of n = 6 replicates (three biological and two technical) ±standard deviation (for 11901 Δ fadD :: tesA strain n = 4 in panels b and c and n = 6 in panel e ). f Detail of WT and engineered PCC 11901 strain cultures centrifuged after 7 days of cultivation. g Lipidomic profiles of the FFA from PCC 11901 and PCC 7002 FFA producer strains: C12:0 (lauric acid), C14:0 (myristic acid), C16:0 (palmitic acid), C18:0 (stearic acid) and C18:1n9t (elaidic acid).
Article Snippet: A synthetic version of the
Techniques: Control, Knock-Out, Comparison, Standard Deviation, Concentration Assay
Journal: Communications Biology
Article Title: Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production
doi: 10.1038/s42003-020-0910-8
Figure Lengend Snippet: FFA production yields achieved by engineered photoautotrophic and heterotrophic hosts having similar modifications.
Article Snippet: A synthetic version of the
Techniques: Modification