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Image Search Results
Journal: Oncology Letters
Article Title: Combined treatment with D-allose, docetaxel and radiation inhibits the tumor growth in an in vivo model of head and neck cancer
doi: 10.3892/ol.2018.7787
Figure Lengend Snippet: Change of mRNA expression after the D-allose and radiation treatment.
Article Snippet: For the Western blot analyses, proteins were separated on 10% SDS-PAGE gels, transferred to nitrocellulose membranes, blocked with 5% (w/v) non-fat dried milk in PBS, and incubated with anti-TXNIP (MBL, Nagoya, Japan),
Techniques: Expressing, Irradiation
Journal: Oncology Letters
Article Title: Combined treatment with D-allose, docetaxel and radiation inhibits the tumor growth in an in vivo model of head and neck cancer
doi: 10.3892/ol.2018.7787
Figure Lengend Snippet: TNF-α, TXNIP and TRX expression after the treatment. Histological features of the skin region. Three weeks after the initial treatment, skin specimens were obtained and fixed with 4% PFA. To observe inflammatory changes, TNF-α antibody was used. (Aa) Normal, saline-treated skin was included as a control (bar, 50 µm). (Ab) Hyperkeratosis and epidermal thickening (arrows) were observed after radiotherapy, with strong TNF-α staining. (Ac) Weak TNF-α staining was observed with D-allose treatment (arrow). (Ad) Radiation-induced epidermal thickening and TNF-α staining were reduced with additional D-allose treatment (arrow). (B) Western blot analysis of TXNIP and TRX expression. Proteins were obtained from: 1, normal skin with saline treatment; 2, normal skin with D-allose treatment for 2 weeks; 3, tumor tissue with saline treatment; 4, tumor tissue with D-allose treatment for 2 weeks; and 5, tumor tissue with D-allose treatment for 3 weeks. TXNIP, thioredoxin interacting protein; TRX, thioredoxin; TNF-α, tumor necrosis factor-α.
Article Snippet: For the Western blot analyses, proteins were separated on 10% SDS-PAGE gels, transferred to nitrocellulose membranes, blocked with 5% (w/v) non-fat dried milk in PBS, and incubated with anti-TXNIP (MBL, Nagoya, Japan),
Techniques: Expressing, Staining, Western Blot