dea nonoate Search Results


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(A) The reactions that comprise the dissimilatory denitrification pathway, or the serial reduction of NO 3 − to nitrogen gas, in bacteria. (B) Growth of the wild-type P. aeruginosa strain PA14 and its derived lasR mutant PA14- lasR ::Gm in a chemically defined medium (PN minimal medium) with and without NO 3 − supplementation, under low oxygen mass-transfer conditions. Results representative of three separate experiments. (C) Nitrate (NO 3 − ) and nitrite (NO 2 − ) concentrations in shaken cultures of the indicated strains grown with LB and 5 mM added nitrate. Results shown are the averages of technical triplicates ±s.d. and are representative of two separate experiments. Slopes for lines fit to each dataset between 4 and 6 minutes were significantly different with p<0.001 for [NO 2 − ] and p<0.02 for [NO 3 − ]. Also shown for reference (inset) are concurrent growth curves for each strain; results representative of two separate experiments. (D) Rates of degradation of added NO· from a mixture of NO· donors <t>(DEANO</t> and ProliNO) by cells pre-grown in a sealed chamber in LB prior to donor addition. Results shown are average ±s.d. for technical duplicates and are representative of two separate experiments. Slopes for lines fit to each dataset between 25 and 100 seconds were significantly different (p<0.02). (E) NO· concentrations in sealed, stirred LB cultures of the indicated strains during growth with 50 mM added NO 3 − . Results are average ±s.d. of three separate experiments. Slopes for lines fit to each dataset between 4 and 9 minutes were significantly different between wild-type and lasR mutant cultures (p<0.02). For all experiments shown, similar results were obtained in at least two separate experiments with the Patient 1 early isolate and its derived lasR mutant (described in ), and complementation with the lasR gene on a plasmid restored wild-type phenotypes (not shown).
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(A) The reactions that comprise the dissimilatory denitrification pathway, or the serial reduction of NO 3 − to nitrogen gas, in bacteria. (B) Growth of the wild-type P. aeruginosa strain PA14 and its derived lasR mutant PA14- lasR ::Gm in a chemically defined medium (PN minimal medium) with and without NO 3 − supplementation, under low oxygen mass-transfer conditions. Results representative of three separate experiments. (C) Nitrate (NO 3 − ) and nitrite (NO 2 − ) concentrations in shaken cultures of the indicated strains grown with LB and 5 mM added nitrate. Results shown are the averages of technical triplicates ±s.d. and are representative of two separate experiments. Slopes for lines fit to each dataset between 4 and 6 minutes were significantly different with p<0.001 for [NO 2 − ] and p<0.02 for [NO 3 − ]. Also shown for reference (inset) are concurrent growth curves for each strain; results representative of two separate experiments. (D) Rates of degradation of added NO· from a mixture of NO· donors <t>(DEANO</t> and ProliNO) by cells pre-grown in a sealed chamber in LB prior to donor addition. Results shown are average ±s.d. for technical duplicates and are representative of two separate experiments. Slopes for lines fit to each dataset between 25 and 100 seconds were significantly different (p<0.02). (E) NO· concentrations in sealed, stirred LB cultures of the indicated strains during growth with 50 mM added NO 3 − . Results are average ±s.d. of three separate experiments. Slopes for lines fit to each dataset between 4 and 9 minutes were significantly different between wild-type and lasR mutant cultures (p<0.02). For all experiments shown, similar results were obtained in at least two separate experiments with the Patient 1 early isolate and its derived lasR mutant (described in ), and complementation with the lasR gene on a plasmid restored wild-type phenotypes (not shown).
Dea/ Nonoate (Dea/No, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) The reactions that comprise the dissimilatory denitrification pathway, or the serial reduction of NO 3 − to nitrogen gas, in bacteria. (B) Growth of the wild-type P. aeruginosa strain PA14 and its derived lasR mutant PA14- lasR ::Gm in a chemically defined medium (PN minimal medium) with and without NO 3 − supplementation, under low oxygen mass-transfer conditions. Results representative of three separate experiments. (C) Nitrate (NO 3 − ) and nitrite (NO 2 − ) concentrations in shaken cultures of the indicated strains grown with LB and 5 mM added nitrate. Results shown are the averages of technical triplicates ±s.d. and are representative of two separate experiments. Slopes for lines fit to each dataset between 4 and 6 minutes were significantly different with p<0.001 for [NO 2 − ] and p<0.02 for [NO 3 − ]. Also shown for reference (inset) are concurrent growth curves for each strain; results representative of two separate experiments. (D) Rates of degradation of added NO· from a mixture of NO· donors <t>(DEANO</t> and ProliNO) by cells pre-grown in a sealed chamber in LB prior to donor addition. Results shown are average ±s.d. for technical duplicates and are representative of two separate experiments. Slopes for lines fit to each dataset between 25 and 100 seconds were significantly different (p<0.02). (E) NO· concentrations in sealed, stirred LB cultures of the indicated strains during growth with 50 mM added NO 3 − . Results are average ±s.d. of three separate experiments. Slopes for lines fit to each dataset between 4 and 9 minutes were significantly different between wild-type and lasR mutant cultures (p<0.02). For all experiments shown, similar results were obtained in at least two separate experiments with the Patient 1 early isolate and its derived lasR mutant (described in ), and complementation with the lasR gene on a plasmid restored wild-type phenotypes (not shown).
2 (N,N Diethylamino) Diazenolate 2 Oxide Dea Nonoate Deano, supplied by AG Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AG Scientific dea nonoate
(A) The reactions that comprise the dissimilatory denitrification pathway, or the serial reduction of NO 3 − to nitrogen gas, in bacteria. (B) Growth of the wild-type P. aeruginosa strain PA14 and its derived lasR mutant PA14- lasR ::Gm in a chemically defined medium (PN minimal medium) with and without NO 3 − supplementation, under low oxygen mass-transfer conditions. Results representative of three separate experiments. (C) Nitrate (NO 3 − ) and nitrite (NO 2 − ) concentrations in shaken cultures of the indicated strains grown with LB and 5 mM added nitrate. Results shown are the averages of technical triplicates ±s.d. and are representative of two separate experiments. Slopes for lines fit to each dataset between 4 and 6 minutes were significantly different with p<0.001 for [NO 2 − ] and p<0.02 for [NO 3 − ]. Also shown for reference (inset) are concurrent growth curves for each strain; results representative of two separate experiments. (D) Rates of degradation of added NO· from a mixture of NO· donors <t>(DEANO</t> and ProliNO) by cells pre-grown in a sealed chamber in LB prior to donor addition. Results shown are average ±s.d. for technical duplicates and are representative of two separate experiments. Slopes for lines fit to each dataset between 25 and 100 seconds were significantly different (p<0.02). (E) NO· concentrations in sealed, stirred LB cultures of the indicated strains during growth with 50 mM added NO 3 − . Results are average ±s.d. of three separate experiments. Slopes for lines fit to each dataset between 4 and 9 minutes were significantly different between wild-type and lasR mutant cultures (p<0.02). For all experiments shown, similar results were obtained in at least two separate experiments with the Patient 1 early isolate and its derived lasR mutant (described in ), and complementation with the lasR gene on a plasmid restored wild-type phenotypes (not shown).
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(A) The reactions that comprise the dissimilatory denitrification pathway, or the serial reduction of NO 3 − to nitrogen gas, in bacteria. (B) Growth of the wild-type P. aeruginosa strain PA14 and its derived lasR mutant PA14- lasR ::Gm in a chemically defined medium (PN minimal medium) with and without NO 3 − supplementation, under low oxygen mass-transfer conditions. Results representative of three separate experiments. (C) Nitrate (NO 3 − ) and nitrite (NO 2 − ) concentrations in shaken cultures of the indicated strains grown with LB and 5 mM added nitrate. Results shown are the averages of technical triplicates ±s.d. and are representative of two separate experiments. Slopes for lines fit to each dataset between 4 and 6 minutes were significantly different with p<0.001 for [NO 2 − ] and p<0.02 for [NO 3 − ]. Also shown for reference (inset) are concurrent growth curves for each strain; results representative of two separate experiments. (D) Rates of degradation of added NO· from a mixture of NO· donors <t>(DEANO</t> and ProliNO) by cells pre-grown in a sealed chamber in LB prior to donor addition. Results shown are average ±s.d. for technical duplicates and are representative of two separate experiments. Slopes for lines fit to each dataset between 25 and 100 seconds were significantly different (p<0.02). (E) NO· concentrations in sealed, stirred LB cultures of the indicated strains during growth with 50 mM added NO 3 − . Results are average ±s.d. of three separate experiments. Slopes for lines fit to each dataset between 4 and 9 minutes were significantly different between wild-type and lasR mutant cultures (p<0.02). For all experiments shown, similar results were obtained in at least two separate experiments with the Patient 1 early isolate and its derived lasR mutant (described in ), and complementation with the lasR gene on a plasmid restored wild-type phenotypes (not shown).
Dea Nonoate, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) The reactions that comprise the dissimilatory denitrification pathway, or the serial reduction of NO 3 − to nitrogen gas, in bacteria. (B) Growth of the wild-type P. aeruginosa strain PA14 and its derived lasR mutant PA14- lasR ::Gm in a chemically defined medium (PN minimal medium) with and without NO 3 − supplementation, under low oxygen mass-transfer conditions. Results representative of three separate experiments. (C) Nitrate (NO 3 − ) and nitrite (NO 2 − ) concentrations in shaken cultures of the indicated strains grown with LB and 5 mM added nitrate. Results shown are the averages of technical triplicates ±s.d. and are representative of two separate experiments. Slopes for lines fit to each dataset between 4 and 6 minutes were significantly different with p<0.001 for [NO 2 − ] and p<0.02 for [NO 3 − ]. Also shown for reference (inset) are concurrent growth curves for each strain; results representative of two separate experiments. (D) Rates of degradation of added NO· from a mixture of NO· donors <t>(DEANO</t> and ProliNO) by cells pre-grown in a sealed chamber in LB prior to donor addition. Results shown are average ±s.d. for technical duplicates and are representative of two separate experiments. Slopes for lines fit to each dataset between 25 and 100 seconds were significantly different (p<0.02). (E) NO· concentrations in sealed, stirred LB cultures of the indicated strains during growth with 50 mM added NO 3 − . Results are average ±s.d. of three separate experiments. Slopes for lines fit to each dataset between 4 and 9 minutes were significantly different between wild-type and lasR mutant cultures (p<0.02). For all experiments shown, similar results were obtained in at least two separate experiments with the Patient 1 early isolate and its derived lasR mutant (described in ), and complementation with the lasR gene on a plasmid restored wild-type phenotypes (not shown).
Diethylammonium(z) 1 (N, N Diethylamino)diazen 1 Ium 1,2 Diolate Dea Nonoate, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) The reactions that comprise the dissimilatory denitrification pathway, or the serial reduction of NO 3 − to nitrogen gas, in bacteria. (B) Growth of the wild-type P. aeruginosa strain PA14 and its derived lasR mutant PA14- lasR ::Gm in a chemically defined medium (PN minimal medium) with and without NO 3 − supplementation, under low oxygen mass-transfer conditions. Results representative of three separate experiments. (C) Nitrate (NO 3 − ) and nitrite (NO 2 − ) concentrations in shaken cultures of the indicated strains grown with LB and 5 mM added nitrate. Results shown are the averages of technical triplicates ±s.d. and are representative of two separate experiments. Slopes for lines fit to each dataset between 4 and 6 minutes were significantly different with p<0.001 for [NO 2 − ] and p<0.02 for [NO 3 − ]. Also shown for reference (inset) are concurrent growth curves for each strain; results representative of two separate experiments. (D) Rates of degradation of added NO· from a mixture of NO· donors <t>(DEANO</t> and ProliNO) by cells pre-grown in a sealed chamber in LB prior to donor addition. Results shown are average ±s.d. for technical duplicates and are representative of two separate experiments. Slopes for lines fit to each dataset between 25 and 100 seconds were significantly different (p<0.02). (E) NO· concentrations in sealed, stirred LB cultures of the indicated strains during growth with 50 mM added NO 3 − . Results are average ±s.d. of three separate experiments. Slopes for lines fit to each dataset between 4 and 9 minutes were significantly different between wild-type and lasR mutant cultures (p<0.02). For all experiments shown, similar results were obtained in at least two separate experiments with the Patient 1 early isolate and its derived lasR mutant (described in ), and complementation with the lasR gene on a plasmid restored wild-type phenotypes (not shown).
Diethylamine Nonoate (Dea/ No), supplied by OXIS International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) The reactions that comprise the dissimilatory denitrification pathway, or the serial reduction of NO 3 − to nitrogen gas, in bacteria. (B) Growth of the wild-type P. aeruginosa strain PA14 and its derived lasR mutant PA14- lasR ::Gm in a chemically defined medium (PN minimal medium) with and without NO 3 − supplementation, under low oxygen mass-transfer conditions. Results representative of three separate experiments. (C) Nitrate (NO 3 − ) and nitrite (NO 2 − ) concentrations in shaken cultures of the indicated strains grown with LB and 5 mM added nitrate. Results shown are the averages of technical triplicates ±s.d. and are representative of two separate experiments. Slopes for lines fit to each dataset between 4 and 6 minutes were significantly different with p<0.001 for [NO 2 − ] and p<0.02 for [NO 3 − ]. Also shown for reference (inset) are concurrent growth curves for each strain; results representative of two separate experiments. (D) Rates of degradation of added NO· from a mixture of NO· donors <t>(DEANO</t> and ProliNO) by cells pre-grown in a sealed chamber in LB prior to donor addition. Results shown are average ±s.d. for technical duplicates and are representative of two separate experiments. Slopes for lines fit to each dataset between 25 and 100 seconds were significantly different (p<0.02). (E) NO· concentrations in sealed, stirred LB cultures of the indicated strains during growth with 50 mM added NO 3 − . Results are average ±s.d. of three separate experiments. Slopes for lines fit to each dataset between 4 and 9 minutes were significantly different between wild-type and lasR mutant cultures (p<0.02). For all experiments shown, similar results were obtained in at least two separate experiments with the Patient 1 early isolate and its derived lasR mutant (described in ), and complementation with the lasR gene on a plasmid restored wild-type phenotypes (not shown).
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(A) The reactions that comprise the dissimilatory denitrification pathway, or the serial reduction of NO 3 − to nitrogen gas, in bacteria. (B) Growth of the wild-type P. aeruginosa strain PA14 and its derived lasR mutant PA14- lasR ::Gm in a chemically defined medium (PN minimal medium) with and without NO 3 − supplementation, under low oxygen mass-transfer conditions. Results representative of three separate experiments. (C) Nitrate (NO 3 − ) and nitrite (NO 2 − ) concentrations in shaken cultures of the indicated strains grown with LB and 5 mM added nitrate. Results shown are the averages of technical triplicates ±s.d. and are representative of two separate experiments. Slopes for lines fit to each dataset between 4 and 6 minutes were significantly different with p<0.001 for [NO 2 − ] and p<0.02 for [NO 3 − ]. Also shown for reference (inset) are concurrent growth curves for each strain; results representative of two separate experiments. (D) Rates of degradation of added NO· from a mixture of NO· donors <t>(DEANO</t> and ProliNO) by cells pre-grown in a sealed chamber in LB prior to donor addition. Results shown are average ±s.d. for technical duplicates and are representative of two separate experiments. Slopes for lines fit to each dataset between 25 and 100 seconds were significantly different (p<0.02). (E) NO· concentrations in sealed, stirred LB cultures of the indicated strains during growth with 50 mM added NO 3 − . Results are average ±s.d. of three separate experiments. Slopes for lines fit to each dataset between 4 and 9 minutes were significantly different between wild-type and lasR mutant cultures (p<0.02). For all experiments shown, similar results were obtained in at least two separate experiments with the Patient 1 early isolate and its derived lasR mutant (described in ), and complementation with the lasR gene on a plasmid restored wild-type phenotypes (not shown).
Dea Nonoate, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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N/A
DEA NONOate is a NO donor It spontaneously dissociates in a pH dependent first order process with a half life of 2 minutes and 16 minutes at 37°C and 22 25°C pH 7 4 respectively
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(A) The reactions that comprise the dissimilatory denitrification pathway, or the serial reduction of NO 3 − to nitrogen gas, in bacteria. (B) Growth of the wild-type P. aeruginosa strain PA14 and its derived lasR mutant PA14- lasR ::Gm in a chemically defined medium (PN minimal medium) with and without NO 3 − supplementation, under low oxygen mass-transfer conditions. Results representative of three separate experiments. (C) Nitrate (NO 3 − ) and nitrite (NO 2 − ) concentrations in shaken cultures of the indicated strains grown with LB and 5 mM added nitrate. Results shown are the averages of technical triplicates ±s.d. and are representative of two separate experiments. Slopes for lines fit to each dataset between 4 and 6 minutes were significantly different with p<0.001 for [NO 2 − ] and p<0.02 for [NO 3 − ]. Also shown for reference (inset) are concurrent growth curves for each strain; results representative of two separate experiments. (D) Rates of degradation of added NO· from a mixture of NO· donors (DEANO and ProliNO) by cells pre-grown in a sealed chamber in LB prior to donor addition. Results shown are average ±s.d. for technical duplicates and are representative of two separate experiments. Slopes for lines fit to each dataset between 25 and 100 seconds were significantly different (p<0.02). (E) NO· concentrations in sealed, stirred LB cultures of the indicated strains during growth with 50 mM added NO 3 − . Results are average ±s.d. of three separate experiments. Slopes for lines fit to each dataset between 4 and 9 minutes were significantly different between wild-type and lasR mutant cultures (p<0.02). For all experiments shown, similar results were obtained in at least two separate experiments with the Patient 1 early isolate and its derived lasR mutant (described in ), and complementation with the lasR gene on a plasmid restored wild-type phenotypes (not shown).

Journal: PLoS Pathogens

Article Title: Nutrient Availability as a Mechanism for Selection of Antibiotic Tolerant Pseudomonas aeruginosa within the CF Airway

doi: 10.1371/journal.ppat.1000712

Figure Lengend Snippet: (A) The reactions that comprise the dissimilatory denitrification pathway, or the serial reduction of NO 3 − to nitrogen gas, in bacteria. (B) Growth of the wild-type P. aeruginosa strain PA14 and its derived lasR mutant PA14- lasR ::Gm in a chemically defined medium (PN minimal medium) with and without NO 3 − supplementation, under low oxygen mass-transfer conditions. Results representative of three separate experiments. (C) Nitrate (NO 3 − ) and nitrite (NO 2 − ) concentrations in shaken cultures of the indicated strains grown with LB and 5 mM added nitrate. Results shown are the averages of technical triplicates ±s.d. and are representative of two separate experiments. Slopes for lines fit to each dataset between 4 and 6 minutes were significantly different with p<0.001 for [NO 2 − ] and p<0.02 for [NO 3 − ]. Also shown for reference (inset) are concurrent growth curves for each strain; results representative of two separate experiments. (D) Rates of degradation of added NO· from a mixture of NO· donors (DEANO and ProliNO) by cells pre-grown in a sealed chamber in LB prior to donor addition. Results shown are average ±s.d. for technical duplicates and are representative of two separate experiments. Slopes for lines fit to each dataset between 25 and 100 seconds were significantly different (p<0.02). (E) NO· concentrations in sealed, stirred LB cultures of the indicated strains during growth with 50 mM added NO 3 − . Results are average ±s.d. of three separate experiments. Slopes for lines fit to each dataset between 4 and 9 minutes were significantly different between wild-type and lasR mutant cultures (p<0.02). For all experiments shown, similar results were obtained in at least two separate experiments with the Patient 1 early isolate and its derived lasR mutant (described in ), and complementation with the lasR gene on a plasmid restored wild-type phenotypes (not shown).

Article Snippet: NO donors DEANO (DEA-NONOate) and ProliNO (Proli-NONOate) were purchased from AG Scientific (San Diego, CA) and SperNO was obtained from CalBiochem (San Diego, CA).

Techniques: Bacteria, Derivative Assay, Mutagenesis, Plasmid Preparation