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The TOH-containing emulgel alleviated activation of the <t>JAK2/STAT3</t> pathway in inflammation and cell proliferation. ( a ) Immunohistochemical staining for JAK2, pJAK2, STAT3, and pSTAT3 on the skin section of all groups was performed. Representative images of skin sections were taken at ×40 magnification (scale bars: 50 μm). ( b , c ) Quantification of JAK2, and STAT3 positive cells in all groups (n = 4). ( d , e ) The mRNA expression of JAK2 and STAT3 . ( f ) Protein expression of JAK2, pJAK2, STAT3 and pSTAT3 evaluated by western blot. ( g , h ) Relative protein expression levels of pJAK2 and pSTAT3 normalized to total JAK2 and STAT3, respectively (n = 4). ( j , k ) Quantification of pJAK2, and pSTAT3 positive cells in all groups (n = 4). ( l , m ) The mRNA expression of BCL2 and CCND1 were analyzed by RT-qPCR (n = 4). The data are presented as the means ± SD. Statistical significance was determined by one-way ANOVA. * p < 0.05, ** p < 0.01, and *** p < 0.001.
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Cell Signaling Technology Inc rabbit anti pstat3 y705
The TOH-containing emulgel alleviated activation of the <t>JAK2/STAT3</t> pathway in inflammation and cell proliferation. ( a ) Immunohistochemical staining for JAK2, pJAK2, STAT3, and pSTAT3 on the skin section of all groups was performed. Representative images of skin sections were taken at ×40 magnification (scale bars: 50 μm). ( b , c ) Quantification of JAK2, and STAT3 positive cells in all groups (n = 4). ( d , e ) The mRNA expression of JAK2 and STAT3 . ( f ) Protein expression of JAK2, pJAK2, STAT3 and pSTAT3 evaluated by western blot. ( g , h ) Relative protein expression levels of pJAK2 and pSTAT3 normalized to total JAK2 and STAT3, respectively (n = 4). ( j , k ) Quantification of pJAK2, and pSTAT3 positive cells in all groups (n = 4). ( l , m ) The mRNA expression of BCL2 and CCND1 were analyzed by RT-qPCR (n = 4). The data are presented as the means ± SD. Statistical significance was determined by one-way ANOVA. * p < 0.05, ** p < 0.01, and *** p < 0.001.
Rabbit Anti Pstat3 Y705, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: Cell Stem Cell

Article Title: Optimized human intestinal organoid model reveals interleukin-22-dependency of paneth cell formation

doi: 10.1016/j.stem.2022.08.002

Figure Lengend Snippet:

Article Snippet: Antibodies used in this study are Phospho STAT3 (Tyr705) (D3A7) XP rabbit antibody, STAT3 (79D7) rabbit antibody, phospho-AKT (ser473) rabbit antibody, Akt (pan) (11E7) rabbit antibody, Phospho-S6 Ribosomal Protein rabbit antibody (Ser235/236), S6 Ribosomal Protein (5G10) rabbit antibody, β-Actin (13E5) rabbit antibody (HRP-conjugated) (Cell Signaling Technology).

Techniques: Recombinant, Reverse Transcription, SYBR Green Assay, Flow Cytometry, Software

The TOH-containing emulgel alleviated activation of the JAK2/STAT3 pathway in inflammation and cell proliferation. ( a ) Immunohistochemical staining for JAK2, pJAK2, STAT3, and pSTAT3 on the skin section of all groups was performed. Representative images of skin sections were taken at ×40 magnification (scale bars: 50 μm). ( b , c ) Quantification of JAK2, and STAT3 positive cells in all groups (n = 4). ( d , e ) The mRNA expression of JAK2 and STAT3 . ( f ) Protein expression of JAK2, pJAK2, STAT3 and pSTAT3 evaluated by western blot. ( g , h ) Relative protein expression levels of pJAK2 and pSTAT3 normalized to total JAK2 and STAT3, respectively (n = 4). ( j , k ) Quantification of pJAK2, and pSTAT3 positive cells in all groups (n = 4). ( l , m ) The mRNA expression of BCL2 and CCND1 were analyzed by RT-qPCR (n = 4). The data are presented as the means ± SD. Statistical significance was determined by one-way ANOVA. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Scientific Reports

Article Title: A tryptophol-containing emulgel ameliorates imiquimod-induced mice psoriasis

doi: 10.1038/s41598-025-04431-4

Figure Lengend Snippet: The TOH-containing emulgel alleviated activation of the JAK2/STAT3 pathway in inflammation and cell proliferation. ( a ) Immunohistochemical staining for JAK2, pJAK2, STAT3, and pSTAT3 on the skin section of all groups was performed. Representative images of skin sections were taken at ×40 magnification (scale bars: 50 μm). ( b , c ) Quantification of JAK2, and STAT3 positive cells in all groups (n = 4). ( d , e ) The mRNA expression of JAK2 and STAT3 . ( f ) Protein expression of JAK2, pJAK2, STAT3 and pSTAT3 evaluated by western blot. ( g , h ) Relative protein expression levels of pJAK2 and pSTAT3 normalized to total JAK2 and STAT3, respectively (n = 4). ( j , k ) Quantification of pJAK2, and pSTAT3 positive cells in all groups (n = 4). ( l , m ) The mRNA expression of BCL2 and CCND1 were analyzed by RT-qPCR (n = 4). The data are presented as the means ± SD. Statistical significance was determined by one-way ANOVA. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: A rabbit monoclonal anti-β-actin (13E5) antibody (#4970S), rabbit monoclonal anti-phosphor-Stat3 (Tyr705) antibody (D3A7), rabbit monoclonal anti-Jak2 antibody (D2E12) were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA).

Techniques: Activation Assay, Immunohistochemical staining, Staining, Expressing, Western Blot, Quantitative RT-PCR