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All (21)
InvivoGen
Hycult Biotech
Coley Pharma
Enzo Biochem
TIB MOLBIOL
Metabion
MilliporeSigma
 

About 25 products / 70 snippets

92 / 100
Bioz Stars
CpG-A DNA (ODN 2216), Human/Mouse
Hycult Biotech  
Catalog#: HC4037
Techniques
 
Article Snippets
"ed by 7-aminoactinomycin-D (7-AAD; 10 µg/ml; Sigma-Aldrich) staining using flow cytometric analysis. For activation, cells were exposed to the TLR9 agonist CpG-A (ODN 2216, 1 μM; Cat. no. HC4037 from Hycult Biotech, Uden, The Netherlands) in the absence or presence of AMA (0.5 μg/ml; Sigma-Aldrich) for 6 h. The optimal concentration of AMA was chosen based on preliminary experiments performed on G"  (see more)
96 / 100
Bioz Stars
ODN 2216
InvivoGen  
Catalog#: TLRL-2216-5
Techniques
 
Article Snippets
"one in (either RPMI 1640, SFEM II, or DC medium). Medium was devoid of growth factors and only supplemented with P/S and IL-3 (20 ng/mL). Cells were subsequently stimulated with agonists directed against TLR7 (R837, tlrl-imq, InvivoGen) or TLR9 (CpG-A 2216, .."
92 / 100
Bioz Stars
CpG-A DNA (ODN 2216), Human/Mouse
Hycult Biotech  
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Catalog#: HC4037
Techniques
 
Article Snippets
"ed by 7-aminoactinomycin-D (7-AAD; 10 µg/ml; Sigma-Aldrich) staining using flow cytometric analysis. For activation, cells were exposed to the TLR9 agonist CpG-A (ODN 2216, 1 μM; Cat. no. HC4037 from Hycult Biotech, Uden, The Netherlands) in the absence or presence of AMA (0.5 μg/ml; Sigma-Aldrich) for 6 h. The optimal concentration of AMA was chosen based on preliminary experiments performed on G"  (see more)
96 / 100
Bioz Stars
ODN 2216
InvivoGen  
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Catalog#: TLRL-2216-5
Techniques
 
Article Snippets
"one in (either RPMI 1640, SFEM II, or DC medium). Medium was devoid of growth factors and only supplemented with P/S and IL-3 (20 ng/mL). Cells were subsequently stimulated with agonists directed against TLR7 (R837, tlrl-imq, InvivoGen) or TLR9 (CpG-A 2216, .."
85 / 100
Bioz Stars
CpG-A ODN 2216
TriLink BioTech  
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Techniques
 
Article Snippets
"SAOS/p53 cell was HLA A2.1 + , p53 deficient human osteosarcoma cell line SAOS-2 transfected with mutated p53 (r to h at position 175), and maintained in 400 ug/ml Geneticin (G418, GIBCO BRL)."  (see more)
85 / 100
Bioz Stars
CpG-A ODN 2216
tebu-bio  
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Techniques
 
Article Snippets
"Healthy pDCs were cultured in the presence of IL-3 (1ng/ml) and stimulated with CpG-A ODN 2216 (Tebu bio) for indicated time."  (see more)
91 / 100
Bioz Stars
CpG-A ODN 2216
Metabion  
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Techniques
 
Article Snippets
"To assess cytokine production by CBMC upon contact with TLR9 ligands, CpG-A ODN 2216 (3 μg/ml; Metabion GmbH, Martinsried, Germany) was employed."  (see more)
80 / 100
Bioz Stars
CpG motifs (CpG ODN) 2216
Vivogen Biotechnology  
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Techniques
 
Article Snippets
"The synthetic oligodeoxynucleotide containing CpG motifs (CpG ODN) 2216 from In-vivoGen (Cayla, Toulouse, France) was used in culture at 12 μg/ml."  (see more)
88 / 100
Bioz Stars
CpG-A ODN 2216 (GsGsGGGACGATCGTCGsGsGsGsGsG)
Coley Pharmaceutical  
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Techniques
 
Article Snippets
"CpG-A ODN 2216 (GsGsGGGACGATCGTCGsGsGsGsGsG) was obtained from Coley Pharmaceuticals (Wellesley, MA); where s represents a phosporothioate linkage."  (see more)
80 / 100
Bioz Stars
CpG-A ODN 2216 Innaxon
Innaxon  
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Techniques
 
Article Snippets
".. LPS from E. Coli 055:85, #L4005-100 mg (Sigma, St Louis Mo., USA), CpG-A ODN 2216 Innaxon #INAX-200-005 (Adipogen, Switzerland), imiquimod VacciGrade #vac-img, HKLM #Tlrl-hklm, (Invivogen, San Diego, Calif., USA). .."  (see more)
91 / 100
Bioz Stars
CpG ODN 2216
Biomol  
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Techniques
 
Article Snippets
"CpG ODN 2216 was obtained from Biomol (Berlin, Germany)."  (see more)
80 / 100
Bioz Stars
ODN CpG 2216
Enzo Biochem / Enzo Life Sciences  
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Techniques
 
Article Snippets
".. 50 μg/ml ODN CpG2007 (Cell Sciences Hycult biotechnology, cat # HC4038 ) or ODN CpG 2216 (Alexis Biochemicals cat #TALX-746-005 ) to induce maturation of DCs."  (see more)
91 / 100
Bioz Stars
CpG-ODN 2216
Thermo Fisher Scientific / Molecular Probes  
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Techniques
 
Article Snippets
"Ready-to-use AlexaFluor 488-labelled CpG-ODN 2216 (type A) was ordered via Molecular Probes/Invitrogen."  (see more)
91 / 100
Bioz Stars
CpG ODN 2216
VBC-Biotech Service  
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Techniques
 
Article Snippets
".. reagent ODN2216, cells were cultured for 24 h (4×10 6 /ml) in the presence of CpG ODN 2216 (12.5 μg/ml; VBC-Biotech, Austria) in RPMI-1640 medium supplemented with 2 mM glutamine .."  (see more)