Journal: Emerging Microbes & Infections

Article Title: Identification and characterization of a novel incompatibility group X3 plasmid carrying bla NDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China

doi: 10.1038/emi.2012.37

Figure Lengend Snippet: Patient demographics, bacterial strains and features of plasmids carrying bla NDM-1

Article Snippet: Plasmid DNA was extracted with QIAGEN Midi Kit (Qiagen, Hilden, Germany) and introduced to competent E. coli DH5α (Invitrogen, Carlsbad, CA, USA) by electroporation, followed by selection of transformants on Luria Bertani agar supplemented with meropenem (0.5 µg/ml).

Techniques:

Journal: Emerging Microbes & Infections

Article Title: Identification and characterization of a novel incompatibility group X3 plasmid carrying bla NDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China

doi: 10.1038/emi.2012.37

Figure Lengend Snippet: Restriction analysis of IncX3 plasmids carrying bla NDM-1 . Plasmids were digested with ( A ) EcoR I and ( B ) Pst I and separated by electrophoresis in 1% agarose. M, GeneRulerTM DNA ladder. The labels above each lane show the strain number, bacterial species origin (EA, E. aerogenes ; KP, K. pneumoniae ; EC, E. coli ; EO, E. cloacae ; CF, C. freundii ) and the geographic source of importation (HN, Hunan; HF, Haifeng; DG, Dongguan; GZ, Guangzhou).

Article Snippet: Plasmid DNA was extracted with QIAGEN Midi Kit (Qiagen, Hilden, Germany) and introduced to competent E. coli DH5α (Invitrogen, Carlsbad, CA, USA) by electroporation, followed by selection of transformants on Luria Bertani agar supplemented with meropenem (0.5 µg/ml).

Techniques: Electrophoresis