Journal: Frontiers in Endocrinology

Article Title: Dapagliflozin Attenuates Renal Tubulointerstitial Fibrosis Associated With Type 1 Diabetes by Regulating STAT1/TGFβ1 Signaling

doi: 10.3389/fendo.2019.00441

Figure Lengend Snippet: Sequences of primers used for quantitative RT-PCR analysis.

Article Snippet: Briefly, 4-μm-thick kidney sections were incubated with anti-FN (1:500), anti-Col IV (1:500), anti-STAT1 (1:500), and anti-TGFβ1 antibody (1:150) overnight at 4°C, followed by incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (BBI Life Science, Shanghai, China).

Techniques: Quantitative RT-PCR

Journal: Frontiers in Endocrinology

Article Title: Dapagliflozin Attenuates Renal Tubulointerstitial Fibrosis Associated With Type 1 Diabetes by Regulating STAT1/TGFβ1 Signaling

doi: 10.3389/fendo.2019.00441

Figure Lengend Snippet: The expressions of STAT1, TGFβ1, FN, and Col IV in non-diabetic adjacent normal kidney tissues (NC), and renal biopsy sections from DN patients. (A) Protein expression levels of STAT1, TGFβ1, FN, and Col IV were quantified by western blot analysis. (B) mRNA expressions levels of STAT1, TGFβ1, FN, and Col IV were quantified by qRT-PCR. * P < 0.05 vs. NC.

Article Snippet: Briefly, 4-μm-thick kidney sections were incubated with anti-FN (1:500), anti-Col IV (1:500), anti-STAT1 (1:500), and anti-TGFβ1 antibody (1:150) overnight at 4°C, followed by incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (BBI Life Science, Shanghai, China).

Techniques: Expressing, Western Blot, Quantitative RT-PCR

Journal: Frontiers in Endocrinology

Article Title: Dapagliflozin Attenuates Renal Tubulointerstitial Fibrosis Associated With Type 1 Diabetes by Regulating STAT1/TGFβ1 Signaling

doi: 10.3389/fendo.2019.00441

Figure Lengend Snippet: Effect of DAPA on renal production of fibrotic markers in diabetic mice. (A) Representative western blots for STAT1, TGF-β1, Col IV, and FN in renal cortical lysates. β-actin was used as internal control. (B) Density ratio of STAT1 to GAPDH mRNA expressions. (C–F) Normalized quantification of western blot data in diabetic mice at different times. * P < 0.05 vs. NC; ** P < 0.05 vs. DM-8W; * & P < 0.05 vs. DM-12W; # P < 0.05 vs. DM-16W. Values are means ± SEM. n = 3.

Article Snippet: Briefly, 4-μm-thick kidney sections were incubated with anti-FN (1:500), anti-Col IV (1:500), anti-STAT1 (1:500), and anti-TGFβ1 antibody (1:150) overnight at 4°C, followed by incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (BBI Life Science, Shanghai, China).

Techniques: Western Blot, Control

Journal: Frontiers in Endocrinology

Article Title: Dapagliflozin Attenuates Renal Tubulointerstitial Fibrosis Associated With Type 1 Diabetes by Regulating STAT1/TGFβ1 Signaling

doi: 10.3389/fendo.2019.00441

Figure Lengend Snippet: Effects of DAPA and Flu on the expression of EMT-related proteins in HK-2 cells. (A) Representative western blots illustrating STAT1, TGF-β1, E-cadherin, α-SMA, and β-actin protein expression in HK2 cells. (B–E) Quantification of the western blot data in HK-2 cells. Statistical analysis was performed with one-way ANOVA. Results are expressed as the mean ± SEM. * P < 0.05 vs. NC, # P < 0.05 vs. HG, #& P < 0.05 vs. HG+DAPA-1.

Article Snippet: Briefly, 4-μm-thick kidney sections were incubated with anti-FN (1:500), anti-Col IV (1:500), anti-STAT1 (1:500), and anti-TGFβ1 antibody (1:150) overnight at 4°C, followed by incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (BBI Life Science, Shanghai, China).

Techniques: Expressing, Western Blot