Journal: Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy

Article Title: Retrospective study on the efficacy of a low-carbohydrate diet for impaired glucose tolerance

doi: 10.2147/DMSO.S62681

Figure Lengend Snippet: Baseline characteristics of the patients

Article Snippet: Serum TGs were measured by the cholesterol oxidation enzyme and enzymatic colorimetry methods (Sekisui Medical H-7700).

Techniques: Control, Clinical Proteomics

Journal: Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy

Article Title: Retrospective study on the efficacy of a low-carbohydrate diet for impaired glucose tolerance

doi: 10.2147/DMSO.S62681

Figure Lengend Snippet: Endpoints at 12 months

Article Snippet: Serum TGs were measured by the cholesterol oxidation enzyme and enzymatic colorimetry methods (Sekisui Medical H-7700).

Techniques: Control, Clinical Proteomics

Journal: Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy

Article Title: Retrospective study on the efficacy of a low-carbohydrate diet for impaired glucose tolerance

doi: 10.2147/DMSO.S62681

Figure Lengend Snippet: ( A ) Profile of fasting plasma glucose; ( B ) glycohemoglobin; ( C ) body weight; ( D ) HOMA-IR; ( E ) LDL cholesterol; ( F ) HDL cholesterol; and ( G ) TGs in each group. Notes: Data points represent the mean ± standard error for each group at the indicated times. The dashed lines and the solid lines represent the LCD group and the control group, respectively. ------- LCD group, —— control group. The HOMA-IR was calculated as fasting insulin (μU/mL) × fasting glucose (mg/dL) ÷ 405. Abbreviations: HDL, high-density lipoprotein; HOMA-IR, homeostasis model of assessment of insulin resistance; LCD, low-carbohydrate diet; LDL, low-density lipoprotein; TGs, triglycerides.

Article Snippet: Serum TGs were measured by the cholesterol oxidation enzyme and enzymatic colorimetry methods (Sekisui Medical H-7700).

Techniques: Clinical Proteomics, Control

Journal: Molecular Neurobiology

Article Title: The Role of Oxidized Cholesterol in Diabetes-Induced Lysosomal Dysfunction in the Brain

doi: 10.1007/s12035-015-9207-1

Figure Lengend Snippet: Cholesterol accumulation within lysosomes of T2D mice. Representative images of hippocampus colabeled with filipin ( green ), which stains cholesterol, and LAMP-1 ( red ), which stains lysosomes, in a – c db+ and d – f db/db mice. Areas of colocalization ( yellow ) demonstrate cholesterol accumulation within lysosomes ( asterisks ). Scale bar represents 10 μm

Article Snippet: In vitro experiments involved treating primary CN with 25 mM of glucose [ ] with or without 5 μM oxidized cholesterol (27-hydroxycholesterol; Medical Isotopes, Inc., Pelham, NH; Prasanthi et al. 2009) for 72 h. Lysosomal destabilization was measured using acridine orange (AO), a lysosomotropic weak base, and metachromatic fluorochrome.

Techniques:

Journal: Molecular Neurobiology

Article Title: The Role of Oxidized Cholesterol in Diabetes-Induced Lysosomal Dysfunction in the Brain

doi: 10.1007/s12035-015-9207-1

Figure Lengend Snippet: CN treatment with oxidized cholesterol and AO Fluroskan analysis of green and red fluorescence at a time 0 and b after treatment with control DMSO or oxidized cholesterol for 72 h. Fluorescence is represented by arbitrary units (A.U.). c , d Images are representative of AO staining following 72 h of c control DMSO or d oxidized cholesterol treatment, where nuclei are stained green and red puncta represent lysosomes. ** p < 0.01 compared with control DMSO. Scale bar represents 50 μm

Article Snippet: In vitro experiments involved treating primary CN with 25 mM of glucose [ ] with or without 5 μM oxidized cholesterol (27-hydroxycholesterol; Medical Isotopes, Inc., Pelham, NH; Prasanthi et al. 2009) for 72 h. Lysosomal destabilization was measured using acridine orange (AO), a lysosomotropic weak base, and metachromatic fluorochrome.

Techniques: Fluorescence, Control, Staining