cd73 nt5e oncogene Search Results


bcl2  (Proteintech)
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Proteintech bcl2
Apoptosis markers in MeT-5A and MPM cells (as Mero-14, Mero-25, IST-Mes2, NCI-H28, and MSTO-211H) after the treatment with fludarabine (F-araA) (A) Caspase activities measured in MeT-5A and MPM cells 24 h after treatment with either vehicle (DMSO) or two different concentrations of F-araA, 10 μM and 1 μM. Data represent the mean ± SEM from three independent experiments. Statistical significance between treatment and vehicle is indicated as P < 0.05*, P < 0.01**, and P < 0.001***. (B) Western blot analysis of BAX and <t>Bcl2,</t> at 48 or 72 h after a treatment with F-araA at 1 μM. An amount of 10 μg of proteins, obtained from cell lysates, was employed. GAPDH or β-Actin were used as protein loading control
Bcl2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Apoptosis markers in MeT-5A and MPM cells (as Mero-14, Mero-25, IST-Mes2, NCI-H28, and MSTO-211H) after the treatment with fludarabine (F-araA) (A) Caspase activities measured in MeT-5A and MPM cells 24 h after treatment with either vehicle (DMSO) or two different concentrations of F-araA, 10 μM and 1 μM. Data represent the mean ± SEM from three independent experiments. Statistical significance between treatment and vehicle is indicated as P < 0.05*, P < 0.01**, and P < 0.001***. (B) Western blot analysis of BAX and Bcl2, at 48 or 72 h after a treatment with F-araA at 1 μM. An amount of 10 μg of proteins, obtained from cell lysates, was employed. GAPDH or β-Actin were used as protein loading control

Journal: Investigational New Drugs

Article Title: Drug-repositioning screening identified fludarabine and risedronic acid as potential therapeutic compounds for malignant pleural mesothelioma

doi: 10.1007/s10637-020-01040-y

Figure Lengend Snippet: Apoptosis markers in MeT-5A and MPM cells (as Mero-14, Mero-25, IST-Mes2, NCI-H28, and MSTO-211H) after the treatment with fludarabine (F-araA) (A) Caspase activities measured in MeT-5A and MPM cells 24 h after treatment with either vehicle (DMSO) or two different concentrations of F-araA, 10 μM and 1 μM. Data represent the mean ± SEM from three independent experiments. Statistical significance between treatment and vehicle is indicated as P < 0.05*, P < 0.01**, and P < 0.001***. (B) Western blot analysis of BAX and Bcl2, at 48 or 72 h after a treatment with F-araA at 1 μM. An amount of 10 μg of proteins, obtained from cell lysates, was employed. GAPDH or β-Actin were used as protein loading control

Article Snippet: The following primary and secondary antibodies, purchased from Proteintech Europe (Manchester, UK), were employed: DCK (17758–1-AP DCK Rabbit Polyclonal antibody), NT5E (12231–1-AP NT5E/CD73 Rabbit Polyclonal antibody,), BAX (50599–2-Ig BAX Rabbit Polyclonal antibody), BCL2 (12789–1-AP BCL2 Rabbit Polyclonal antibody), β-actin (60008–1-Ig beta Actin Mouse Monoclonal antibody), GAPDH (60004–1-Ig Mouse Monoclonal antibody), Peroxidase-conjugated Affinipure Goat Anti-Mouse IgG(H + L) (SA00001–1) and Peroxidase-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) (SA00001–2).

Techniques: Western Blot