Journal: Nature Communications

Article Title: An immune checkpoint score system for prognostic evaluation and adjuvant chemotherapy selection in gastric cancer

doi: 10.1038/s41467-020-20260-7

Figure Lengend Snippet: Multi-colour immunofluorescence staining of anti-CKpan and CD45 antibodies with CD155, NECTIN2, CEACAM1, HMGB1, SIGLEC6 or CD44 antibodies. Scale bar = 200 μm.

Article Snippet: Multiple immunofluorescence staining was performed to identify the expression of six indicators (NECTIN2 (95333S, CST, 1 : 100), CEACAM1 (44464S, CST, 1 : 200), HMGB1 (ab18256, Abcam, 1 : 800), SIGLEC6 (ab38581, Abcam, 1 : 100), CD44 (3570S, CST, 1 : 50), CD155 (81254S, CST, 1 : 200)), CD45 (ab10558, Abcam, 1 : 100) and panCK (ab7753, Abcam, 1 : 100) in 135 types of GC tissues.

Techniques: Immunofluorescence, Staining

Journal: International Journal of Molecular Sciences

Article Title: SOCS1 Inhibits IL-6-Induced CD155 Overexpression in Lung Adenocarcinoma

doi: 10.3390/ijms252212141

Figure Lengend Snippet: IL-6 and CD155 expression in lung adenocarcinoma data from TCGA. ( A ) IL-6 expression did not change among lung adenocarcinoma stages. CD155 (PVR) expression in stage III cases ( n = 84) was higher than that in early stages I ( n = 275) and II ( n = 121). Patients with stage IV ( n = 25). The minimum, 25th percentile, median, 75th percentile, and maximum values are shown. ( B ) Correlation between the expression of IL-6 and PVR (CD155) in the lung adenocarcinoma cohort ( n = 505). ( C ) Correlation between IL-6 and CD155 among the clinical stages of the lung adenocarcinoma cohort.

Article Snippet: Soluble CD155 was quantified using the Human CD155/PVR/Poliovirus Receptor ELISA Kit, PicoKine (Cat. No. EK1831, Boster Biological Technology, Pleasanton, CA, USA).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: SOCS1 Inhibits IL-6-Induced CD155 Overexpression in Lung Adenocarcinoma

doi: 10.3390/ijms252212141

Figure Lengend Snippet: Effect of IL-6 stimulation on CD155 expression on lung adenocarcinoma cell lines. ( A ) IL-6 stimulation induced CD155 mRNA expression in the tested cell lines. ( B ) Upon IL-6 stimulation, CD155 protein expression increased in A-549 and NCI-H1437 cells, but no change was detected in the advanced stages 3B1A and NCI-H1573 cell lines. ( C ) Results show that CD155 concentration was augmented only in the supernatant of the IL-6-stimulated A-549 cell line. ( D ) Addition of the JAK2 inhibitor AG490 prevents IL-6-induced CD155 overexpression in the A-549 and NCI-H1437 cell lines. ( A , C ) are shown as mean + SD. * p < 0.05, ** p < 0.01.

Article Snippet: Soluble CD155 was quantified using the Human CD155/PVR/Poliovirus Receptor ELISA Kit, PicoKine (Cat. No. EK1831, Boster Biological Technology, Pleasanton, CA, USA).

Techniques: Expressing, Concentration Assay, Over Expression

Journal: International Journal of Molecular Sciences

Article Title: SOCS1 Inhibits IL-6-Induced CD155 Overexpression in Lung Adenocarcinoma

doi: 10.3390/ijms252212141

Figure Lengend Snippet: SOCS1 negatively regulates CD155 expression. ( A ) Comparison of CD155 expression between lung adenocarcinoma patients from TCGA with high and low SOCS1 expression. ( B ) Western blot analysis showed that the 3B1A and NCI-H1573 (advanced tumor stages) cell lines showed high SOCS1 expression compared to the A-549 and NCI-H1437 (tumor stage I) cell lines, and this expression was not significantly affected by IL-6 stimulation. ( C ) Western blotting and ( D ) immunofluorescence show that SOCS1 knockdown enables the 3B1A and NCI-H1573 cell lines to augment CD155 expression upon IL-6 stimulation. CD155 (green) and nuclear staining (blue). Magnification 400×.

Article Snippet: Soluble CD155 was quantified using the Human CD155/PVR/Poliovirus Receptor ELISA Kit, PicoKine (Cat. No. EK1831, Boster Biological Technology, Pleasanton, CA, USA).

Techniques: Expressing, Comparison, Western Blot, Immunofluorescence, Knockdown, Staining

Journal: International Journal of Molecular Sciences

Article Title: SOCS1 Inhibits IL-6-Induced CD155 Overexpression in Lung Adenocarcinoma

doi: 10.3390/ijms252212141

Figure Lengend Snippet: IL-6 and CD155 expression in patients with LUAD. ( A ) Representative micrographs of cases with high (top) and low (bottom) IL-6 and CD155 staining and cases with low (top) and high (bottom) CD8+ T cell infiltration in lung adenocarcinoma biopsies. IL-6 showed cytoplasmic staining, while CD155 was found in the membrane and cytoplasm of the tumor cells. Magnification 200×. ( B ) Positive correlation between the relative expression of IL-6 and CD155 in lung adenocarcinoma in tissues ( n = 39). ( C ) Negative correlation between CD155 expression and CD8+ T-cell infiltration in lung adenocarcinoma tissues. ( D ) Correlation between serum IL-6 and CD155 concentrations in patients with primary lung adenocarcinoma ( n = 31).

Article Snippet: Soluble CD155 was quantified using the Human CD155/PVR/Poliovirus Receptor ELISA Kit, PicoKine (Cat. No. EK1831, Boster Biological Technology, Pleasanton, CA, USA).

Techniques: Expressing, Staining, Membrane