cd14 pc5 5 Search Results


90
Becton Dickinson anti-cd14 pc5.5 m5e2
PMN-MDSC discriminate active TB and LTBI. (A) Gating strategy used to identify MDSC: in the morphological gate (FSC/SSC) we excluded debris, then we gated Lin-/HLA DRlow/- cells. In this gate, we selected CD11b+CD33+ cells (MDSC). Then, the expression of <t>CD14,</t> CD15 is shown. The percentage of Lin-/HLA-DR-/CD11b+/CD33+ has been calculated in the morphological gate. (B) PMN-MDSC frequency in active TB , LTBI and HD . Results are shown as Box and Whiskers. The Kruskall-Wallis test (with Dunn’s multiple comparison posttest) was applied. (C) Receiver operating characteristic (ROC) curve for the PMN-MDSC frequency. Right panel TB vs HD, left panel TB vs LTBI. (D) Receiver operating characteristic (ROC) curve for the Mo/Ly ratio. Right panel TB vs HD, left panel TB vs LTBI. PMN-MDSC: polymorphonuclear-myeloid-derived suppressor cells; TB, tuberculosis; LTBI, latent tuberculosis infection; HD, healthy donors.
Anti Cd14 Pc5.5 M5e2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd14+pc5+5/anti+cd14/pmc08107479-62-22-36
Average 90 stars, based on 1 article reviews
anti-cd14 pc5.5 m5e2 - by Bioz Stars, 2026-09
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PMN-MDSC discriminate active TB and LTBI. (A) Gating strategy used to identify MDSC: in the morphological gate (FSC/SSC) we excluded debris, then we gated Lin-/HLA DRlow/- cells. In this gate, we selected CD11b+CD33+ cells (MDSC). Then, the expression of CD14, CD15 is shown. The percentage of Lin-/HLA-DR-/CD11b+/CD33+ has been calculated in the morphological gate. (B) PMN-MDSC frequency in active TB , LTBI and HD . Results are shown as Box and Whiskers. The Kruskall-Wallis test (with Dunn’s multiple comparison posttest) was applied. (C) Receiver operating characteristic (ROC) curve for the PMN-MDSC frequency. Right panel TB vs HD, left panel TB vs LTBI. (D) Receiver operating characteristic (ROC) curve for the Mo/Ly ratio. Right panel TB vs HD, left panel TB vs LTBI. PMN-MDSC: polymorphonuclear-myeloid-derived suppressor cells; TB, tuberculosis; LTBI, latent tuberculosis infection; HD, healthy donors.

Journal: Frontiers in Immunology

Article Title: PMN-MDSC Frequency Discriminates Active Versus Latent Tuberculosis and Could Play a Role in Counteracting the Immune-Mediated Lung Damage in Active Disease

doi: 10.3389/fimmu.2021.594376

Figure Lengend Snippet: PMN-MDSC discriminate active TB and LTBI. (A) Gating strategy used to identify MDSC: in the morphological gate (FSC/SSC) we excluded debris, then we gated Lin-/HLA DRlow/- cells. In this gate, we selected CD11b+CD33+ cells (MDSC). Then, the expression of CD14, CD15 is shown. The percentage of Lin-/HLA-DR-/CD11b+/CD33+ has been calculated in the morphological gate. (B) PMN-MDSC frequency in active TB , LTBI and HD . Results are shown as Box and Whiskers. The Kruskall-Wallis test (with Dunn’s multiple comparison posttest) was applied. (C) Receiver operating characteristic (ROC) curve for the PMN-MDSC frequency. Right panel TB vs HD, left panel TB vs LTBI. (D) Receiver operating characteristic (ROC) curve for the Mo/Ly ratio. Right panel TB vs HD, left panel TB vs LTBI. PMN-MDSC: polymorphonuclear-myeloid-derived suppressor cells; TB, tuberculosis; LTBI, latent tuberculosis infection; HD, healthy donors.

Article Snippet: Evaluation of MDSC percentage was performed using 0.5 x 10 6 PBMC stained with anti-CD15 FITC (clone MMA), anti-CD33 PE (clone WM53), anti-CD14 PC5.5 (clone M5E2), anti-CD11b PE-Cy7 (clone M1/70), anti-CD16 pacific blue (clone 3G8) from BD Biosciences, and anti-HLA-DR ECD (clone IMMU-357), cocktail of antibodies anti-CD3 APC-A700 (clone UCHT1), -CD56 APC-A700 (N901), -CD19 APC-A700 (clone J3-119) (Lin) from Beckman Coulter.

Techniques: Expressing, Derivative Assay, Infection

PMN-MDSC frequency correlates with the CD14++CD16- monocyte proportion. (A) Gating strategy used to identify monocyte subsets: in the morphological gate (FSC/SSC) we excluded debris gate (A), and morphologically identified monocytes gate (B). In gate B, we selected CD14++CD16, CD14++CD16+, and CD14-CD16++ monocytes. The percentage of monocyte subsets was calculated in the gate (A) Correlation between PMN-MDSC frequency and CD14++CD16 (B) , CD14++CD16+ (C) , and CD14-CD16++ (D) monocytes from active TB and LTBI subjects. The Spearman test was applied to evaluate correlations. The p <0.05 was considered statistically significant.

Journal: Frontiers in Immunology

Article Title: PMN-MDSC Frequency Discriminates Active Versus Latent Tuberculosis and Could Play a Role in Counteracting the Immune-Mediated Lung Damage in Active Disease

doi: 10.3389/fimmu.2021.594376

Figure Lengend Snippet: PMN-MDSC frequency correlates with the CD14++CD16- monocyte proportion. (A) Gating strategy used to identify monocyte subsets: in the morphological gate (FSC/SSC) we excluded debris gate (A), and morphologically identified monocytes gate (B). In gate B, we selected CD14++CD16, CD14++CD16+, and CD14-CD16++ monocytes. The percentage of monocyte subsets was calculated in the gate (A) Correlation between PMN-MDSC frequency and CD14++CD16 (B) , CD14++CD16+ (C) , and CD14-CD16++ (D) monocytes from active TB and LTBI subjects. The Spearman test was applied to evaluate correlations. The p <0.05 was considered statistically significant.

Article Snippet: Evaluation of MDSC percentage was performed using 0.5 x 10 6 PBMC stained with anti-CD15 FITC (clone MMA), anti-CD33 PE (clone WM53), anti-CD14 PC5.5 (clone M5E2), anti-CD11b PE-Cy7 (clone M1/70), anti-CD16 pacific blue (clone 3G8) from BD Biosciences, and anti-HLA-DR ECD (clone IMMU-357), cocktail of antibodies anti-CD3 APC-A700 (clone UCHT1), -CD56 APC-A700 (N901), -CD19 APC-A700 (clone J3-119) (Lin) from Beckman Coulter.

Techniques: