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This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant Cynomolgus CD14 Catalog 90174 C08H B3Y6B8 Met1 Met344 and
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Isotype Note IgG2a kappa Host Species Note Mouse Reactivity Note Human Cow Pig Sheep
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Image Search Results
Journal: Frontiers in immunology
Article Title: T-cell deficiency and hyperinflammatory monocyte responses associate with Mycobacterium avium complex lung disease.
doi: 10.3389/fimmu.2022.1016038
Figure Lengend Snippet: FIGURE 1 Individuals with MAC disease have infrequent Mav- or mycobacteria-specific responses. (A) Total magnitude of response (sum of IFNg, IL-5 and IL-17) against peptide pools, Mav and Mtb lysates as SFC per 106 cultured PBMC as determined by Fluorospot. Each point represents one participant (MACDZ, n = 10 in blue; IGRA+HC, n = 10 in teal), median ± interquartile range is shown. Two-tailed Mann-Whitney test, comparisons without a p-value indicated were not significant. Limit of detection is 20 SFC. (B) Frequency of PDL1+OX40+ CD4 T-cells in response to peptide pools, Mav and Mtb lysates. Each point represents one participant (MACDZ, n = 10; IGRA+HC, n = 10), median ± interquartile range is shown. Two-tailed Mann-Whitney test. CD4 T-cells were gated as CD3+CD4+CD8-CD19-CD14- in the live singlet gate of PBMC.
Article Snippet: In addition, other cells were stained with a mixture of the following antibodies: CD4-APCef780 (RPA-T4, eBiosciences), CD3-AF700 (UCHT1, BD Pharmigen), CD8a-BV650 (RPA-T8, Biolegend), CD19-PECy7 (HIB19,
Techniques: Cell Culture, Two Tailed Test, MANN-WHITNEY