Journal: Molecular Metabolism

Article Title: Loss of Tbk1 kinase activity protects mice from diet-induced metabolic dysfunction

doi: 10.1016/j.molmet.2018.06.007

Figure Lengend Snippet: Tbk1 Δ/ Δ mice have less inflammation relative to Tbk1 +/ + mice on HFD . (A) Representative 20x images and quantification of cd11b/c stained subcutaneous white adipose tissue from Tbk1 Δ/Δ and Tbk1 +/+ mice after 10 weeks on ND or HFD (n = 4–8 mice/group). Scale bar indicates 200 μM and ‘CLS’ refers to crown like structures. (B) mRNA expression of genes encoding CD11c, F4/80, TNFα, IL-6 and IL-12 in subcutaneous WAT of Tbk1 Δ/Δ and Tbk1 +/+ mice fed with HFD as indicated (n = 4–6 mice/group). (C) Liver tissue lysates from 14-week-old HFD-fed Tbk1 Δ/Δ and Tbk1 +/+ mice were immunoblotted with antibodies against IL-6 and IL-1β. β-actin and GAPDH were used as internal loading controls. Indicated cytokines from WAT (D) and liver tissue (E) lysates of HFD-fed Tbk1 +/+ and Tbk1 Δ/Δ mice were measured by Bio-Rad multiplex array (n = 4–8 mice/group for WAT, n = 8–12 mice/group for liver). All mice are from 129S5 background. Results are representative of mean +/− SEM. Statistical analysis by Student's t -test. * p < 0.05, ** p < 0.01.

Article Snippet: Sections for immunohistochemical analysis were blocked with 20% aquablock and incubated with rabbit anti-cd11b/c (Novus Biologicals, NB110-40766) in blocking solution (5% BSA in TBS with 0.05% tween) at 4 °C overnight.

Techniques: Staining, Expressing, Multiplex Assay

Journal: BMC Molecular and Cell Biology

Article Title: Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages

doi: 10.1186/s12860-020-00331-9

Figure Lengend Snippet: Antibodies used in the study

Article Snippet: CD11b/c Biotin (OX-42) , CD11b/c, CR3 , Cedarlane , CL042B , 2 μg/ml.

Techniques: Concentration Assay, Flow Cytometry, Control, Staining, FACS

Journal: Frontiers in Physiology

Article Title: Localization and Interaction between Kinin B1 Receptor and NADPH Oxidase in the Vascular System of Diabetic Rats

doi: 10.3389/fphys.2017.00861

Figure Lengend Snippet: Representative fluorescent photomicrographs (A) of STZ and control aortic sections showing immunofluorescent staining for B1R (Green color), macrophages (φ, Red color), vascular smooth muscle cells (VSMC, Red color), and endothelial cells (Endo, Red color). Merge pictures (as Orange-Yellow color) show the co-localization of B1R with macrophages and VSMC, and some endothelial cells in STZ-aorta. No co-localization was seen in control-aorta between B1R and the three cell types. Scale bars = 0.1 mm. Data are representative of a minimum of four aortic sections per rat from four controls and four STZ-diabetic rats. Bar Graph (B) illustrates semi-quantitative B1R immunofluorescent intensity in macrophages, VSMC and endothelial cells in STZ- and Control-aortae. Data are mean ± SEM obtained from four rats per group; * P < 0.0001 STZ vs. Control.

Article Snippet: The following primary antibodies were used: home-made rabbit Anti-B1R (Lin et al., ; Lacoste et al., ); mouse Anti-RECA-1 (NB-10064647, Novus Biologicals, Littleton, CO, USA); mouse Anti-rat CD11b/c recognition of macrophages (Cedarlane CL042AP, Burlington, ON, Canada); mouse Anti-alpha smooth muscle Actin (abcam ab7817, Toronto, ON, Canada) while goat Anti-NOX1 (SC-5821) and goat Anti-NOX2 (Anti gp91-phox, SC-5827) were from Santa Cruz Biotechnology, CA, USA.

Techniques: Control, Staining

Journal: Frontiers in Physiology

Article Title: Localization and Interaction between Kinin B1 Receptor and NADPH Oxidase in the Vascular System of Diabetic Rats

doi: 10.3389/fphys.2017.00861

Figure Lengend Snippet: Representative fluorescent photomicrographs (A) of STZ and control aortic sections showing immunofluorescent staining for NOX1 (Blue color), macrophages (φ, Red color), vascular smooth muscle cells (VSMC, Red color), and endothelial cells (Endo, Red color). While merge labeling (as Blue-Purple color) was absent in control-aorta, it was obvious in the STZ-aorta on each cell type. Scale bars = 0.1 mm. Data are representative of a minimum of four aortic sections per rat from four controls and four STZ-diabetic rats. Bar Graph (B) illustrates semi-quantitative NOX1 immunofluorescent intensity in macrophages, VSMC, and endothelial cells in STZ- and Control-aortae. Data are mean ± SEM obtained from four rats per group; * P < 0.0001 STZ vs. Control.

Article Snippet: The following primary antibodies were used: home-made rabbit Anti-B1R (Lin et al., ; Lacoste et al., ); mouse Anti-RECA-1 (NB-10064647, Novus Biologicals, Littleton, CO, USA); mouse Anti-rat CD11b/c recognition of macrophages (Cedarlane CL042AP, Burlington, ON, Canada); mouse Anti-alpha smooth muscle Actin (abcam ab7817, Toronto, ON, Canada) while goat Anti-NOX1 (SC-5821) and goat Anti-NOX2 (Anti gp91-phox, SC-5827) were from Santa Cruz Biotechnology, CA, USA.

Techniques: Control, Staining, Labeling

Journal: Frontiers in Physiology

Article Title: Localization and Interaction between Kinin B1 Receptor and NADPH Oxidase in the Vascular System of Diabetic Rats

doi: 10.3389/fphys.2017.00861

Figure Lengend Snippet: Representative fluorescent photomicrographs (A) of STZ and control aortic sections showing immunofluorescent staining for NOX2 (Blue color), macrophages (φ, Red color), vascular smooth muscle cells (VSMC, Red color), and endothelial cells (Endo, Red color). While merge labeling (as Blue-Purple color) was small in control-aorta, it was obvious in the STZ-aorta on each cell type. Scale bars = 0.1 mm. Data are representative of a minimum of four aortic sections per rat from four controls and four STZ-diabetic rats. Bar Graph (B) illustrates semi-quantitative NOX2 immunofluorescent intensity in macrophages, VSMC, and endothelial cells in STZ- and Control-aortae. Data are mean ± SEM obtained from four rats per group; * P < 0.0001 STZ vs. Control.

Article Snippet: The following primary antibodies were used: home-made rabbit Anti-B1R (Lin et al., ; Lacoste et al., ); mouse Anti-RECA-1 (NB-10064647, Novus Biologicals, Littleton, CO, USA); mouse Anti-rat CD11b/c recognition of macrophages (Cedarlane CL042AP, Burlington, ON, Canada); mouse Anti-alpha smooth muscle Actin (abcam ab7817, Toronto, ON, Canada) while goat Anti-NOX1 (SC-5821) and goat Anti-NOX2 (Anti gp91-phox, SC-5827) were from Santa Cruz Biotechnology, CA, USA.

Techniques: Control, Staining, Labeling

Journal: Frontiers in Physiology

Article Title: Localization and Interaction between Kinin B1 Receptor and NADPH Oxidase in the Vascular System of Diabetic Rats

doi: 10.3389/fphys.2017.00861

Figure Lengend Snippet: Representative fluorescent photomicrographs of STZ and control aortic sections showing merge immunofluorescent staining (as White color dots) between B1R and NOX1 ( Upper-Left ) or B1R and NOX2 ( Upper-Right ) on the same cell type (macrophages and VSMC and to some extent on endothelial cells) in STZ-aorta. Such co-localization did not occur in control-aorta. Scale bars = 0.1 mm. Data are representative of a minimum of four aortic sections per rat from four controls and four STZ-diabetic rats. Bottom Bar Graphs illustrate semi-quantitative immunofluorescent intensity for B1R with NOX1 (Left) and NOX2 (Right) on the same cell type in STZ- and Control-aortae. Data are mean ± SEM obtained from four rats per group; * P < 0.0001 STZ vs. Control.

Article Snippet: The following primary antibodies were used: home-made rabbit Anti-B1R (Lin et al., ; Lacoste et al., ); mouse Anti-RECA-1 (NB-10064647, Novus Biologicals, Littleton, CO, USA); mouse Anti-rat CD11b/c recognition of macrophages (Cedarlane CL042AP, Burlington, ON, Canada); mouse Anti-alpha smooth muscle Actin (abcam ab7817, Toronto, ON, Canada) while goat Anti-NOX1 (SC-5821) and goat Anti-NOX2 (Anti gp91-phox, SC-5827) were from Santa Cruz Biotechnology, CA, USA.

Techniques: Control, Staining

Journal: Frontiers in Physiology

Article Title: Localization and Interaction between Kinin B1 Receptor and NADPH Oxidase in the Vascular System of Diabetic Rats

doi: 10.3389/fphys.2017.00861

Figure Lengend Snippet: Microphotographs of immunolocalization of B1R with NOX1 (A) and NOX2 (B) on macrophages, endothelial cells and VSMC by confocal microscopy (40 X). Shown are immunolabeling for B1R (green), macrophages (φ, Red color), endothelial cells (Endo, Red color), vascular smooth muscle cells (VSMC, Red color), and NOX1 or NOX2 (Blue color) in STZ- and Control-aorta. Note that B1R co-localized (as White color dots) with NOX1 and NOX2 on macrophages, VSMC, and rarely on endothelial cells in STZ-aorta. Triple immunocolocalization was not seen in Control-aorta. Data are representative of a minimum of four aortic sections per rat from four Controls and four STZ-diabetic rats.

Article Snippet: The following primary antibodies were used: home-made rabbit Anti-B1R (Lin et al., ; Lacoste et al., ); mouse Anti-RECA-1 (NB-10064647, Novus Biologicals, Littleton, CO, USA); mouse Anti-rat CD11b/c recognition of macrophages (Cedarlane CL042AP, Burlington, ON, Canada); mouse Anti-alpha smooth muscle Actin (abcam ab7817, Toronto, ON, Canada) while goat Anti-NOX1 (SC-5821) and goat Anti-NOX2 (Anti gp91-phox, SC-5827) were from Santa Cruz Biotechnology, CA, USA.

Techniques: Confocal Microscopy, Immunolabeling, Control

Journal: Frontiers in Physiology

Article Title: Localization and Interaction between Kinin B1 Receptor and NADPH Oxidase in the Vascular System of Diabetic Rats

doi: 10.3389/fphys.2017.00861

Figure Lengend Snippet: Representative fluorescent photomicrographs (A) of STZ and control popliteal sections showing immunofluorescent staining for B1R (Green color), macrophages (φ, Red color), vascular smooth muscle cells (VSMC, Red color), and endothelial cells (Endo, Red color). Merge pictures (as Orange-Yellow color) show the co-localization of B1R on macrophages, VSMC, and endothelial cells in STZ-popliteal artery. B1R was also slightly expressed on VSMC and endothelial cells in the control resistance artery. Scale bars = 0.1 mm. Data are representative of a minimum of four popliteal sections per rat from four controls and four STZ-diabetic rats. Bar Graph (B) illustrates semi-quantitative B1R immunofluorescent intensity in macrophages, VSMC, and endothelial cells in STZ- and Control-popliteal artery. Data are mean ± SEM obtained from four rats per group; * P < 0.0001 STZ vs. Control.

Article Snippet: The following primary antibodies were used: home-made rabbit Anti-B1R (Lin et al., ; Lacoste et al., ); mouse Anti-RECA-1 (NB-10064647, Novus Biologicals, Littleton, CO, USA); mouse Anti-rat CD11b/c recognition of macrophages (Cedarlane CL042AP, Burlington, ON, Canada); mouse Anti-alpha smooth muscle Actin (abcam ab7817, Toronto, ON, Canada) while goat Anti-NOX1 (SC-5821) and goat Anti-NOX2 (Anti gp91-phox, SC-5827) were from Santa Cruz Biotechnology, CA, USA.

Techniques: Control, Staining

Journal: Frontiers in Physiology

Article Title: Localization and Interaction between Kinin B1 Receptor and NADPH Oxidase in the Vascular System of Diabetic Rats

doi: 10.3389/fphys.2017.00861

Figure Lengend Snippet: Representative fluorescent photomicrographs (A) of STZ and control renal cortex showing immunofluorescent staining for B1R (Green color), macrophages (φ, Red color), vascular smooth muscle cells (VSMC, Red color), and endothelial cells (Endo, Red color). Merge pictures (as Orange-Yellow color) show the co-localization of B1R on macrophages, VSMC, and endothelial cells in STZ-arteries (A) and/or glomeruli (G). B1R was not coexpressed in the same control structures except in glomeruli. Scale bars = 0.1 mm. Data are representative of a minimum of four renal sections per rat from four controls and four STZ-diabetic rats. Bar Graph (B) illustrates semi-quantitative B1R immunofluorescent intensity in macrophages, VSMC and endothelial cells in STZ- and Control-renal cortex. Data are mean ± SEM obtained from four rats per group; * P < 0.0001 STZ vs. Control.

Article Snippet: The following primary antibodies were used: home-made rabbit Anti-B1R (Lin et al., ; Lacoste et al., ); mouse Anti-RECA-1 (NB-10064647, Novus Biologicals, Littleton, CO, USA); mouse Anti-rat CD11b/c recognition of macrophages (Cedarlane CL042AP, Burlington, ON, Canada); mouse Anti-alpha smooth muscle Actin (abcam ab7817, Toronto, ON, Canada) while goat Anti-NOX1 (SC-5821) and goat Anti-NOX2 (Anti gp91-phox, SC-5827) were from Santa Cruz Biotechnology, CA, USA.

Techniques: Control, Staining