c43 de3 hosts Search Results


bl21(de3) competent e. coli  (New England Biolabs)
99
New England Biolabs bl21(de3) competent e. coli
Bl21(de3) Competent E. Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c43+de3+hosts/BL21(DE3)+Competent+E%2E+coli/custom%40c2527%4010045489
Average 99 stars, based on 1 article reviews
bl21(de3) competent e. coli - by Bioz Stars, 2026-09
99/100 stars
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e. coli host strain c43 (de3)  (Avidis Inc)
90
Avidis Inc e. coli host strain c43 (de3)
Evaluation of enzyme activity and antigen in <t>E</t> <t>coli</t> crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight <t>C43</t> <t>(DE3)</t> cultures expressing the indicated VLCAD proteins in pET-21a.
E. Coli Host Strain C43 (De3), supplied by Avidis Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c43+de3+hosts/e++coli+c41++de3++cells/pmc02702680-85-19-25
Average 90 stars, based on 1 article reviews
e. coli host strain c43 (de3) - by Bioz Stars, 2026-09
90/100 stars
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e. coli bl21(de3  (Promega)
90
Promega e. coli bl21(de3
Evaluation of enzyme activity and antigen in <t>E</t> <t>coli</t> crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight <t>C43</t> <t>(DE3)</t> cultures expressing the indicated VLCAD proteins in pET-21a.
E. Coli Bl21(de3, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c43+de3+hosts/e++coli+bl21+de3+plyss/10__1074_slash_jbc__m314165200-45-6-7
Average 90 stars, based on 1 article reviews
e. coli bl21(de3 - by Bioz Stars, 2026-09
90/100 stars
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c43(de3  (Avidis Inc)
90
Avidis Inc c43(de3
Evaluation of enzyme activity and antigen in <t>E</t> <t>coli</t> crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight <t>C43</t> <t>(DE3)</t> cultures expressing the indicated VLCAD proteins in pET-21a.
C43(de3, supplied by Avidis Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c43+de3+hosts/c43+de3/pmc04641660-89-14-15
Average 90 stars, based on 1 article reviews
c43(de3 - by Bioz Stars, 2026-09
90/100 stars
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host strains like  (Lucigen Corp)
86
Lucigen Corp host strains like
Evaluation of enzyme activity and antigen in <t>E</t> <t>coli</t> crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight <t>C43</t> <t>(DE3)</t> cultures expressing the indicated VLCAD proteins in pET-21a.
Host Strains Like, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c43+de3+hosts/host+like+strains/pm42092412-58-12-24
Average 86 stars, based on 1 article reviews
host strains like - by Bioz Stars, 2026-09
86/100 stars
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bl21 de3  (Addgene inc)
93
Addgene inc bl21 de3
Evaluation of enzyme activity and antigen in <t>E</t> <t>coli</t> crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight <t>C43</t> <t>(DE3)</t> cultures expressing the indicated VLCAD proteins in pET-21a.
Bl21 De3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c43+de3+hosts/BL21(DE3)-R3+(Bacterial+strain+%2326241)/pm40378355-1817-10-36
Average 93 stars, based on 1 article reviews
bl21 de3 - by Bioz Stars, 2026-09
93/100 stars
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pet-21a(+) expression constructs  (Avidis Inc)
90
Avidis Inc pet-21a(+) expression constructs
Evaluation of enzyme activity and antigen in E coli crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight C43 (DE3) cultures expressing the indicated VLCAD proteins in <t>pET-21a.</t>
Pet 21a(+) Expression Constructs, supplied by Avidis Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c43+de3+hosts/pet+21a+expression+plasmid/pmc02702680-151-1-19
Average 90 stars, based on 1 article reviews
pet-21a(+) expression constructs - by Bioz Stars, 2026-09
90/100 stars
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e. coli strain c43 (de3)  (Promega)
90
Promega e. coli strain c43 (de3)
Evaluation of enzyme activity and antigen in E coli crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight C43 (DE3) cultures expressing the indicated VLCAD proteins in <t>pET-21a.</t>
E. Coli Strain C43 (De3), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c43+de3+hosts/e++coli+strain+c43++de3/pm30926919-283-28-33
Average 90 stars, based on 1 article reviews
e. coli strain c43 (de3) - by Bioz Stars, 2026-09
90/100 stars
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streptovaricins producing wild-type strain  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection streptovaricins producing wild-type strain
Evaluation of enzyme activity and antigen in E coli crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight C43 (DE3) cultures expressing the indicated VLCAD proteins in <t>pET-21a.</t>
Streptovaricins Producing Wild Type Strain, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c43+de3+hosts/streptovaricins/pmc07481197__41467_2020_18336_MOESM1_ESM-196-46-42
Average 90 stars, based on 1 article reviews
streptovaricins producing wild-type strain - by Bioz Stars, 2026-09
90/100 stars
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hiload 16 60 superdex pg 200 column  (Cytiva Europe)
97
Cytiva Europe hiload 16 60 superdex pg 200 column
Evaluation of enzyme activity and antigen in E coli crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight C43 (DE3) cultures expressing the indicated VLCAD proteins in <t>pET-21a.</t>
Hiload 16 60 Superdex Pg 200 Column, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c43+de3+hosts/HiLoad+16%2F600+Superdex+200+pg/pmc08682760-105-35-43
Average 97 stars, based on 1 article reviews
hiload 16 60 superdex pg 200 column - by Bioz Stars, 2026-09
97/100 stars
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t4 dna ligase  (New England Biolabs)
99
New England Biolabs t4 dna ligase
Evaluation of enzyme activity and antigen in E coli crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight C43 (DE3) cultures expressing the indicated VLCAD proteins in <t>pET-21a.</t>
T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c43+de3+hosts/T4+DNA+Ligase/custom%40m0202%4019205689
Average 99 stars, based on 1 article reviews
t4 dna ligase - by Bioz Stars, 2026-09
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Image Search Results


Evaluation of enzyme activity and antigen in E coli crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight C43 (DE3) cultures expressing the indicated VLCAD proteins in pET-21a.

Journal:

Article Title: EXPRESSION AND CHARACTERIZATION OF MUTATIONS IN HUMAN VERY LONG-CHAIN ACYL-COA DEHYDROGENASE USING A PROKARYOTIC SYSTEM

doi: 10.1016/j.ymgme.2007.01.013

Figure Lengend Snippet: Evaluation of enzyme activity and antigen in E coli crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight C43 (DE3) cultures expressing the indicated VLCAD proteins in pET-21a.

Article Snippet: Expression and purification of recombinant VLCADs The pET-21a(+) expression constructs containing the various VLCAD sequences were transformed into the E. coli host strain C43 (DE3) (Avidis, Saint Beauzire, France).

Techniques: Activity Assay, Recombinant, Mutagenesis, Purification, Expressing

Evaluation of enzyme activity and antigen in E coli crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight C43 (DE3) cultures expressing the indicated VLCAD proteins in pET-21a.

Journal:

Article Title: EXPRESSION AND CHARACTERIZATION OF MUTATIONS IN HUMAN VERY LONG-CHAIN ACYL-COA DEHYDROGENASE USING A PROKARYOTIC SYSTEM

doi: 10.1016/j.ymgme.2007.01.013

Figure Lengend Snippet: Evaluation of enzyme activity and antigen in E coli crude lysates containing the recombinant VLCAD and various mutant VLCAD enzymes. (A) Activity was measured using C16:0-CoA as substrate. Bars represent means and standard deviations of triplicate assays. Note that mutant R429W had minimal detectable activity (0.4 ± 0.01 mU/mg) while R573W had no detectable activity. (B) VLCAD antigen was detected using a polyclonal antibody raised against the purified recombinant ΔEx3 VLCAD shown in lane 1. The top band represents VLCAD while the bottom band is due to non-specific immunoreactivity with an E coli protein. Lanes 2 through 8 contain 25 μg of E coli crude lysate from induced overnight C43 (DE3) cultures expressing the indicated VLCAD proteins in pET-21a.

Article Snippet: The pET-21a(+) expression constructs containing the various VLCAD sequences were transformed into the E. coli host strain C43 (DE3) (Avidis, Saint Beauzire, France).

Techniques: Activity Assay, Recombinant, Mutagenesis, Purification, Expressing