Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: MTFR2-Mediated Fission Drives Fatty Acid and Mitochondrial Co-Transfer from Hepatic Stellate Cells to Tumor Cells Fueling Oncogenesis.

doi: 10.1002/advs.202416419

Figure Lengend Snippet: Figure 3. Activated HSCs enhance fatty acid (FA) synthesis and transfer to cancer cells, promoting proliferation. A) Western blot showing the ACC1 and CPT1A level of Huh7 cells and Huh7 cells cocultured with aHSCs (n = 3). B) Schematic of the experimental setup for FA chase pulse assay. HSCs were incubated in complete media (CM) with red fluorescent FA (Red C12) for 16 h (“pulse”). Following this, HSCs were cultured for an additional 72 h in CM or tumor-conditioned media (TCM) without the labeled FA (“chase”). Mitochondria and lipid drops (LDs) were stained before imaging. C,D) FA localization was assayed as described in (A) and chased in CM or TCM. C) LDs were labeled using BODIPY 493/503 (gray) and mitochondria were labeled using MitoTracker Far Red (green). Scale bar = 25 μm. D) Relative cellular localization of Red C12 was quantified by Pearson’s coefficient analysis

Article Snippet: For FA transfer assays, HSCs were preloaded with 1 × 10−3 m Red C12 (BODIPY 558/568 C12, MCE, Catalog No. HY-138226) in CM for 16 h, then rigorously washed three times with CM and equilibrated for 1 h to remove extracellular dye aggregates.

Techniques: Western Blot, Incubation, Cell Culture, Labeling, Staining, Imaging

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: MTFR2-Mediated Fission Drives Fatty Acid and Mitochondrial Co-Transfer from Hepatic Stellate Cells to Tumor Cells Fueling Oncogenesis.

doi: 10.1002/advs.202416419

Figure Lengend Snippet: Figure 5. Complementary roles of FAs transfer and mitochondrial transport in supporting tumor cell survival. A) Representative confocal images of Huh7 cells cocultured with aHSCs under L-778123 and NTL treatment. Maximum intensity projections (MIP) of confocal Z-stacks showing Huh7 cells (CellTrace green, green) cocultured with aHSCs pre-labeled with Red C12 fluorescent FA (red) (Upper panels). Corresponding 3D surface renderings (Imaris) of the boxed regions (Lower panels). Scale bars: 15 μm. B) Graph showing the fluorescence intensity of FAs in CellTrace green labeled Huh7 cells (Data are represented as mean ± SD, n = 6, at least 50 recipient cells analyzed per replicate, one-way ANOVA was performed). C) Flow cytometry analysis of fluorescence intensity of FAs in FITC-positive Huh7 cells. D) Confocal images showing the transportation of mitochondria from aHSCs to Huh7 cells. (Upper panels) MIP: Confocal Z-stacks of cocultured MitoTracker-labeled mitochondria in aHSCs (red) and CellTrace green-labeled Huh7 cells (green), with ActinTracker (cyan) highlighting tunneling nanotubes (TNTs). (Lower panels) 3D surface reconstruction (Imaris). Scale bars: 15 μm. E) Quantification of fluorescence intensity from Figure D, showing the uptake of HSC-derived mitochondria by Huh7 cells (Data are represented as mean ± SD, n = 6, at least 50 recipient cells analyzed per replicate, one-way ANOVA was performed). F) Fluorescence histograms of Huh7 cells (labeled

Article Snippet: For FA transfer assays, HSCs were preloaded with 1 × 10−3 m Red C12 (BODIPY 558/568 C12, MCE, Catalog No. HY-138226) in CM for 16 h, then rigorously washed three times with CM and equilibrated for 1 h to remove extracellular dye aggregates.

Techniques: Labeling, Flow Cytometry, Derivative Assay, Fluorescence

Journal: BMC Infectious Diseases

Article Title: Harmine acts as a quorum sensing inhibitor decreasing the virulence and antibiotic resistance of Pseudomonas aeruginosa

doi: 10.1186/s12879-024-09639-9

Figure Lengend Snippet: Structures of molecules and data of molecular docking. The structures of autoinducers C12-HSL ( a ), C4-HSL ( b ), and PQS ( c ); and the structure of Harmine ( d ). The molecular docking data ( e ) of autoinducers and Harmine with LasR , RhlR , and PqsR , respectively

Article Snippet: Harmine (CSA: 442-51-3), C12-HSL (CAS: 168982-69-2), C4-HSL (CAS: 67605–85-0) and PQS (CAS: 108985-27-9) were purchased from MedChemExpress (LLC, Shanghai, China).

Techniques: