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Image Search Results
Journal:
Article Title: Bifidobacterium longum Requires a Fructokinase (Frk; ATP: d -Fructose 6-Phosphotransferase, EC 2.7.1.4) for Fructose Catabolism
doi: 10.1128/JB.186.19.6515-6525.2004
Figure Lengend Snippet: Bacterial strains, plasmids, and oligonucleotides used for this study
Article Snippet:
Techniques: Plasmid Preparation, Sequencing, Expressing
Journal:
Article Title: Bifidobacterium longum Requires a Fructokinase (Frk; ATP: d -Fructose 6-Phosphotransferase, EC 2.7.1.4) for Fructose Catabolism
doi: 10.1128/JB.186.19.6515-6525.2004
Figure Lengend Snippet: Purification of the fructokinase Frk. Samples (1 μg of protein) were separated in a sodium dodecyl sulfate-10% polyacrylamide gel and stained with Coomassie brilliant blue R-250. Lane 1, molecular mass standard; lane 2, negative control [a crude extract of E. coli BL21 (DE3) containing pET28a(+)]; lane 3, crude extract of IPTG-induced cells of E. coli BL21 (DE3) containing pFrk; lane 4, eluate with sonication buffer containing 150 mM imidazole from cobalt-based affinity column; lane 5, purified Frk after elimination of the six-His tag by thrombin cleavage.
Article Snippet:
Techniques: Purification, Staining, Negative Control, Sonication, Affinity Column