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Image Search Results
Journal: Regenerative Therapy
Article Title: Evaluation of human keratinocyte sheets transplanted onto porcine excised esophagus after submucosal dissection in an ex vivo model
doi: 10.1016/j.reth.2020.11.004
Figure Lengend Snippet: Endoscopic balloon-based cell sheet transplantation device. (A) Pass the air tube through an endoscopic biopsy channel. (B) Connect cell sheet carrier and air tube. Inflate the balloon by injecting 20 cc of air with syringe. (C) The normal state of the balloon. (D) The contracted state of the balloon. (E) The expanded state of the balloon.
Article Snippet: The endoscopic balloon-based
Techniques: Transplantation Assay
Journal: Regenerative Therapy
Article Title: Evaluation of human keratinocyte sheets transplanted onto porcine excised esophagus after submucosal dissection in an ex vivo model
doi: 10.1016/j.reth.2020.11.004
Figure Lengend Snippet: (A) Normal Human Epidermal Keratinocytes (NHEK) cultured on temperature-responsive culture inserts were harvested as a cell sheet after incubation at 20 °C for 30 min. (B) Ex vivo porcine ESD model (1) Utilizing freshly excised slaughterhouse porcine tissue. (2) A longitudinal incision with Cooper scissors is made from the oral side of the esophagus to the gastric side (3,4) Dissection of about 10 cm of submucosa with Cooper scissors. (C) Transplantation of untreated and expanded cell sheets (control group and expanded group). (D) Expanded group (1) cell sheet is placed on the deflated balloon device. (2) Inflate the balloon for 30 s by injecting 20 cc of air to inflate then deflate repeatedly in sequence 3 times total.
Article Snippet: The endoscopic balloon-based
Techniques: Cell Culture, Incubation, Ex Vivo, Dissection, Transplantation Assay, Sequencing
Journal: Regenerative Therapy
Article Title: Evaluation of human keratinocyte sheets transplanted onto porcine excised esophagus after submucosal dissection in an ex vivo model
doi: 10.1016/j.reth.2020.11.004
Figure Lengend Snippet: Endoscopic transplantation of keratinocyte sheets to an ex vivo porcine submucosal dissection model. A) Balloon inflation method. (1) cell sheet placed on the deflated balloon device with a some culture medium. (2) Unfold the sheet by expanding the balloon. (3) Center the sheet on the balloon with forceps. (4) Shrink the sheet by deflating the balloon. (5) Ensure sheet remains unfolded in the center of the balloon by Inflating the balloon. (6) Store the cell sheet in the device by air suction. (B) Modified balloon method. (1) Unfold the sheet in the center of the expanded balloon with forceps. (2) Store the cell sheet in the device using balloon deflation. (C) Endoscopic views of a cell sheet transplantation. (1) Delivering the cell sheet to the ulcer site through the over-tube. Cell sheet faces the ulcer site by insertion/extraction and rotation of the device (2,3) Attach the cell sheet to the ulcer site for about 30 s by inflating the balloon. (4) Deflate the balloon. (5) Sheet transplant findings under normal observation. (6) Sheet transplant findings under NBI (narrow band imaging) observation.
Article Snippet: The endoscopic balloon-based
Techniques: Transplantation Assay, Ex Vivo, Dissection, Modification, Imaging