Journal: ACS Omega

Article Title: Silencing of LncRNA XIST Suppressed Tumor Growth and Metastasis in Papillary Thyroid Carcinoma by Modulating miR-204/FN1 Axis

doi: 10.1021/acsomega.5c00390

Figure Lengend Snippet: Protein expression of EMT-related proteins (E-cadherin, N-cadherin, and Vimentin) in PTC cells with different transfections using Western blot. * p < 0.05, ** p < 0.01 compared with the blank group; # p < 0.05, ## p < 0.01 compared with the siNC + inhibitor NC group; and p < 0.05, and p < 0.01 compared with the si-lncRNA + inhibitor NC group; @ p < 0.05, @@ p < 0.01 compared with the si-lncRNA + miRNA inhibitor group; $ p < 0.05, $$ p < 0.01 compared with the siNC + miRNA inhibitor group; % p < 0.05, %%: p < 0.01 compared with the si-FN1 + inhibitor NC group.

Article Snippet: After blocking with 5% skim milk at 37 °C for 2 h, the membranes were incubated with anti-E-cadherin antibody (1:1000, Proteintech, Wuhan, China), anti-N-cadherin antibody (1:500, Boster, Wuhan, China), anti-Vimentin antibody (1:1000, Proteintech), and anti-GAPDH antibody (1:5000, Proteintech) overnight at 4 °C.

Techniques: Expressing, Transfection, Western Blot

Journal: Journal of neurological disorders & stroke

Article Title: Neuroendocrine Targeting of Tissue Plasminogen Activator (t-PA)

doi:

Figure Lengend Snippet: Microtiter wells were coated with t-PA and the interaction with 125I-CgA was determined as described in Experimental Procedures in the presence of the indicated concentrations of anti-t-PA mAb's directed against either the EGF-finger domain (open circles), kringle 2 (closed squares), kringle 1 (open squares) or normal mouse IgG (closed circles). Specific binding is shown.

Article Snippet: Anti-t-PA monoclonal antibodies (mAB’s) (#3700 against the EGF/Finger domain, #3704 against kringle 2 and #372 against kringle 1) were purchased from American Diagnostica (Greenwich, CT).

Techniques: Binding Assay