Journal: PLoS ONE

Article Title: Functional Characterization of the Type III Secretion ATPase SsaN Encoded by Salmonella Pathogenicity Island 2

doi: 10.1371/journal.pone.0094347

Figure Lengend Snippet: ( A ) Salmonella wild-type, ΔssaN mutant, ΔssaN mutant complemented with cloned ssaN , and ssaD mutant strains were grown in LPM medium (pH 5.8). Protein samples from equal numbers of bacteria were analyzed by Western blotting using an anti-SseB antibody for the secreted protein fraction (Sup) and whole cell lysates (Lysate). ( B ) Salmonella wild-type and mutant strains that harbored pSseJ-2HA plasmid were grown in LPM medium (pH 5.8). Protein samples from equal numbers of bacteria were subjected to Western blot analysis to detect SeeJ-2HA and SseB in secreted protein fractions (Sup) and in whole cell lysates (Lysate). ( C ) Salmonella wild-type, ΔssaN mutant, ΔssaN mutant complemented with cloned ssaN , and ΔssaN mutant complemented with point mutated ssaN R192G strains were grown in LPM medium (pH 5.8). Protein samples from equal numbers of bacteria were analyzed by Western blotting with an anti-SseB antibody for the secreted protein fraction (Sup) and whole cell lysates (Lysate). ( D ) Confocal immunofluorescence analysis of HeLa cells infected with Salmonella strains that expressed SseJ-2HA. Infected cells were fixed at 8 h after infection. Bacteria were labeled with a rabbit anti- Salmonella LPS O4 antibody ( red ), and a mouse anti-HA antibody was used to detect HA-fusion proteins ( green ). All these experiments were repeated independently three times with similar results. Representative images are shown.

Article Snippet: Rabbit polyclonal anti- Salmonella LPS O4 antibody (Denka Seiken) was used at a dilution of 1∶5,000.

Techniques: Mutagenesis, Clone Assay, Bacteria, Western Blot, Plasmid Preparation, Immunofluorescence, Infection, Labeling

Journal: Sensors (Basel, Switzerland)

Article Title: Characterizations of Anti-Alpha-Fetoprotein-Conjugated Magnetic Nanoparticles Associated with Alpha-Fetoprotein for Biomedical Applications

doi: 10.3390/s17092018

Figure Lengend Snippet: Pictures showing ( a ) biofunctionalized Fe 3 O 4 -anti-AFP; ( b ) AFPs; ( c ) magnetic cluster composed of Fe 3 O 4 -anti-AFP-AFP.

Article Snippet: The BMNPs were Fe 3 O 4 -anti-AFP (MF-AFP-0060, MagQu Co. Ltd., New Taipei City, Taiwan), and the biotarget was AFP, which is a biomarker for diagnosing HCC.

Techniques:

Journal: Pharmaceutics

Article Title: Biomimetic Cell-Derived Nanoparticles: Emerging Platforms for Cancer Immunotherapy

doi: 10.3390/pharmaceutics15071821

Figure Lengend Snippet: Application of biomimetic cell-derived nanoparticles in ACT.

Article Snippet: NK92MI cell membrane , Anti-CD56 antibodies modified Fe 3 O 4 nanoparticles , Good biocompatibility, magnetic targeting, rapid clearance by macrophages , -- , K562, B16F10, and H22 cells, NK92 cell possessing a broader-spectrum anti-cancer activity , The biomimetic delivery system up-regulated the secretion of granzyme B and IFN-γ at the tumor site, which thereby significantly improved the antitumor effect. , [ ] .

Techniques: Membrane, Produced, Amplification, Activation Assay, Modification, Activity Assay