Journal: PLoS Pathogens

Article Title: Enhanced inflammation in New Zealand white rabbits when MERS-CoV reinfection occurs in the absence of neutralizing antibody

doi: 10.1371/journal.ppat.1006565

Figure Lengend Snippet: Rabbits were inoculated intranasally with EMC/2012 strain of MERS-CoV (green arrows) and tissue samples were collected for viral titration and histopathology at necropsy (blue arrows). Three rabbits were necropsied at each time point. Numbers indicate days since virus administration for primary, (secondary), or [tertiary] infections.

Article Snippet: For immunohistochemistry (IHC) mouse anti-MERS nucleocapsid protein (NP) antibody (Biorbyt) was added at a dilution of 1:100, followed by biotinylated horse anti-mouse immunoglobulin G (IgG; Vector Laboratories) at a dilution of 1:200.

Techniques: Titration, Histopathology, Virus

Journal: PLoS Pathogens

Article Title: Enhanced inflammation in New Zealand white rabbits when MERS-CoV reinfection occurs in the absence of neutralizing antibody

doi: 10.1371/journal.ppat.1006565

Figure Lengend Snippet: Viral RNA titers in the nasal turbinates (A) and lungs (B) of rabbits following infection with either 10 3 or 10 5 TCID 50 of EMC/2012 strain of MERS-CoV through day 5 after infection. In a separate experiment, viral RNA titers were determined in the nasal turbinates (C) and lungs (D) following infection with 10 6.5 TCID 50 . n = 3 rabbits per group. Statistical significance was determined using one-way ANOVA with Tukey’s multiple comparisons test. p values *<0.05, ***<0.001.

Article Snippet: For immunohistochemistry (IHC) mouse anti-MERS nucleocapsid protein (NP) antibody (Biorbyt) was added at a dilution of 1:100, followed by biotinylated horse anti-mouse immunoglobulin G (IgG; Vector Laboratories) at a dilution of 1:200.

Techniques: Infection

Journal: PLoS Pathogens

Article Title: Enhanced inflammation in New Zealand white rabbits when MERS-CoV reinfection occurs in the absence of neutralizing antibody

doi: 10.1371/journal.ppat.1006565

Figure Lengend Snippet: Images show H&E (left) and IHC against the MERS-CoV N protein (right) following infection with 10 5 TCID 50 (A,D), 10 3 TCID 50 (B,E), or a media only control (C,F). All images at 10x, (bar equivalent to 100μm) with 40x insets (bar equivalent to 20μm). Images shown are from day 3 post-infection for all groups.

Article Snippet: For immunohistochemistry (IHC) mouse anti-MERS nucleocapsid protein (NP) antibody (Biorbyt) was added at a dilution of 1:100, followed by biotinylated horse anti-mouse immunoglobulin G (IgG; Vector Laboratories) at a dilution of 1:200.

Techniques: Infection, Control

Journal: PLoS Pathogens

Article Title: Enhanced inflammation in New Zealand white rabbits when MERS-CoV reinfection occurs in the absence of neutralizing antibody

doi: 10.1371/journal.ppat.1006565

Figure Lengend Snippet: Viral RNA titers in the lungs of rabbits following reinfection with EMC/2012 (A). Images show H&E (left) and IHC for the MERS-CoV N protein (right) following reinfection for the 10 3// 10 5 TCID 50 reinfection group (B,E) and 10 5// 10 5 TCID 50 reinfection group (C,F). The 10 5 TCID 50 // media control group was included to demonstrate that the observed inflammation was not residual from the primary infection (D,G). n = 3 rabbits per group. All images at 10x, (bar equivalent to 100μm) with 40x inset (bar equivalent to 20μm). Images from day 3 post-infection.

Article Snippet: For immunohistochemistry (IHC) mouse anti-MERS nucleocapsid protein (NP) antibody (Biorbyt) was added at a dilution of 1:100, followed by biotinylated horse anti-mouse immunoglobulin G (IgG; Vector Laboratories) at a dilution of 1:200.

Techniques: Control, Infection

Journal: PLoS Pathogens

Article Title: Enhanced inflammation in New Zealand white rabbits when MERS-CoV reinfection occurs in the absence of neutralizing antibody

doi: 10.1371/journal.ppat.1006565

Figure Lengend Snippet: Viral RNA titers in the lungs following tertiary infection with EMC/2012 strain (A). Images show H&E staining (B) and IHC with an antibody against the MERS-CoV N protein (C) in the 10 5// 10 5// 10 5 TCID 50 group. Images are representative of all rabbits following tertiary infection. All images at 10x, (bar equivalent to 100μm) with 40x inset (bar equivalent to 20μm). n = 3 rabbits per group. Statistical significance was determined using one-way ANOVA with Tukey’s multiple comparisons test. Images from day 3 post-infection. p values **<0.01, ***<0.001.

Article Snippet: For immunohistochemistry (IHC) mouse anti-MERS nucleocapsid protein (NP) antibody (Biorbyt) was added at a dilution of 1:100, followed by biotinylated horse anti-mouse immunoglobulin G (IgG; Vector Laboratories) at a dilution of 1:200.

Techniques: Infection, Staining

Journal: PLoS Pathogens

Article Title: Enhanced inflammation in New Zealand white rabbits when MERS-CoV reinfection occurs in the absence of neutralizing antibody

doi: 10.1371/journal.ppat.1006565

Figure Lengend Snippet: Viral RNA titers in the lungs upon infection with 10 5 TCID 50 of MERS-CoV in rabbits either previously infected with a low dose of MERS-CoV (10 3 TCID 50 ) four weeks prior or naïve rabbits following PT of post-infection sera at either a full dose or 1:10 dilution (A). Images show the H&E staining (left) and IHC with an antibody against the MERS-CoV N protein (right) following infection for the 10 3// 10 5 TCID 50 (reinfection) group (B,E), the group that received passive transfer of undiluted post-infection serum (C, F), and the group that received passive transfer of post-infection serum at 1:10 dilution (D,G). n = 3 rabbits per group. Images from day 3 post-infection at 10x, bar equivalent to 100μm.

Article Snippet: For immunohistochemistry (IHC) mouse anti-MERS nucleocapsid protein (NP) antibody (Biorbyt) was added at a dilution of 1:100, followed by biotinylated horse anti-mouse immunoglobulin G (IgG; Vector Laboratories) at a dilution of 1:200.

Techniques: Infection, Staining

Journal: PLoS Pathogens

Article Title: Enhanced inflammation in New Zealand white rabbits when MERS-CoV reinfection occurs in the absence of neutralizing antibody

doi: 10.1371/journal.ppat.1006565

Figure Lengend Snippet: ELISA against C3a protein in rabbit lung homogenates (A) show an increase in C3a levels present during reinfection compared to primary infection. Immunofluorescence images show MERS-CoV N antigen (green) and complement (red) following secondary infection (B) and primary infection (C). Images from day 3 post-infection at 40x, bar equivalent to 20μm. n = 3 rabbits per group. Statistical significance was determined using one-way ANOVA with Dunnett’s multiple comparisons test. p values *<0.05.

Article Snippet: For immunohistochemistry (IHC) mouse anti-MERS nucleocapsid protein (NP) antibody (Biorbyt) was added at a dilution of 1:100, followed by biotinylated horse anti-mouse immunoglobulin G (IgG; Vector Laboratories) at a dilution of 1:200.

Techniques: Enzyme-linked Immunosorbent Assay, Infection, Immunofluorescence

Journal: STAR Protocols

Article Title: Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials

doi: 10.1016/j.xpro.2022.101362

Figure Lengend Snippet: Surface antibody master mix, titrated for staining of up to 3 M cells per condition

Article Snippet: Anti-Human CD195/CCR5 (NP-6G4)-171Yb—50 Tests (1:50) , Fluidigm , Cat#3171017A.

Techniques: Staining

Journal: STAR Protocols

Article Title: Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials

doi: 10.1016/j.xpro.2022.101362

Figure Lengend Snippet:

Article Snippet: Anti-Human CD195/CCR5 (NP-6G4)-171Yb—50 Tests (1:50) , Fluidigm , Cat#3171017A.

Techniques: Recombinant, Saline, Staining, Blocking Assay, Isolation, Software, Cell Counting, Cytometry