Journal: Frontiers in Medicine

Article Title: ACADL and ADH1B signify ketone body metabolic reprogramming in osteoarthritic synovium: insights from bioinformatics and animal model studies

doi: 10.3389/fmed.2026.1700784

Figure Lengend Snippet: The evaluation of clinical predicting value, functional signaling pathways, and molecular crosstalk of ACADL and ADH1B . (A) The nomogram constructed based on the two biomarkers. “Points” indicates the individual scores corresponding to the values of each variable; “Total Points” represent the sum of all individual scores, which corresponds to the “Odds” axis at the bottom and can be used to estimate the predicted probability of the target event (e.g., disease occurrence). (B,C) The predictive performance of the nomogram model was jointly validated by ROC analysis (B) and calibration curves (C) , with results demonstrating its strong discriminative ability and calibration accuracy. (D,E) The primary pathways identified in GSEA of ACADL (D) and ADH1B (E) . (F,G) GeneMANIA-based interaction networks of ACADL (F) and ADH1B (G) .

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline with Tween-20 (TBST) for 60 min at room temperature, incubated with ACADL polyclonal antibody (Proteintech, Wuhan, China, 17442-1-AP, 1:1,000) or ADH1B polyclonal antibody (Affinity, Liyang, China, DF12809, 1:2,000) or Vinculin monoclonal antibody (Proteintech, Wuhan, China, 66305-2-Ig, 1:10,000) overnight at 4°C, washed with TBST, then incubated with Goat anti-rabbit IgG H&L (HRP) secondary antibody (Abcam, Cambridge, United Kingdom, ab6721, 1:10,000) or Rabbit anti-mouse IgG H&L (HRP) secondary antibody (Abcam, Cambridge, United Kingdom, ab6728, 1:20,000) for 60 min at room temperature.

Techniques: Functional Assay, Protein-Protein interactions, Construct

Journal: Frontiers in Medicine

Article Title: ACADL and ADH1B signify ketone body metabolic reprogramming in osteoarthritic synovium: insights from bioinformatics and animal model studies

doi: 10.3389/fmed.2026.1700784

Figure Lengend Snippet: Pharmacological prediction and molecular docking of ACADL and ADH1B . (A) Drug-biomarker regulatory network. Orange nodes represent the two biomarkers, while purple nodes represent the predicted drugs. (B,C) Molecular docking simulations of ACADL -progesterone (B) and ADH1B-fomepizole (C).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline with Tween-20 (TBST) for 60 min at room temperature, incubated with ACADL polyclonal antibody (Proteintech, Wuhan, China, 17442-1-AP, 1:1,000) or ADH1B polyclonal antibody (Affinity, Liyang, China, DF12809, 1:2,000) or Vinculin monoclonal antibody (Proteintech, Wuhan, China, 66305-2-Ig, 1:10,000) overnight at 4°C, washed with TBST, then incubated with Goat anti-rabbit IgG H&L (HRP) secondary antibody (Abcam, Cambridge, United Kingdom, ab6721, 1:10,000) or Rabbit anti-mouse IgG H&L (HRP) secondary antibody (Abcam, Cambridge, United Kingdom, ab6728, 1:20,000) for 60 min at room temperature.

Techniques: Biomarker Discovery

Journal: Frontiers in Medicine

Article Title: ACADL and ADH1B signify ketone body metabolic reprogramming in osteoarthritic synovium: insights from bioinformatics and animal model studies

doi: 10.3389/fmed.2026.1700784

Figure Lengend Snippet: ACADL and ADH1B expression in murine synovium. (A–D) The representative images of immunohistochemistry staining of ACADL (A) and ADH1B (B) in the synovium of mouse model and statistical analysis. (E) Western blot analysis of ACADL and ADH1B of mouse model. CTRL, control; OA, osteoarthritis. Data were presented as Mean ± SD (B,D,E). * p < 0.05, *** p < 0.001, **** p < 0.0001, non-paired Student t -test. Scale bar = 200μm (left) or 50μm (right).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline with Tween-20 (TBST) for 60 min at room temperature, incubated with ACADL polyclonal antibody (Proteintech, Wuhan, China, 17442-1-AP, 1:1,000) or ADH1B polyclonal antibody (Affinity, Liyang, China, DF12809, 1:2,000) or Vinculin monoclonal antibody (Proteintech, Wuhan, China, 66305-2-Ig, 1:10,000) overnight at 4°C, washed with TBST, then incubated with Goat anti-rabbit IgG H&L (HRP) secondary antibody (Abcam, Cambridge, United Kingdom, ab6721, 1:10,000) or Rabbit anti-mouse IgG H&L (HRP) secondary antibody (Abcam, Cambridge, United Kingdom, ab6728, 1:20,000) for 60 min at room temperature.

Techniques: Expressing, Immunohistochemistry, Staining, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Notoginsenoside R1 Regulates Ischemic Myocardial Lipid Metabolism by Activating the AKT/mTOR Signaling Pathway

doi: 10.3389/fphar.2022.905092

Figure Lengend Snippet: Validation of key differential protein expression in the lipid metabolism–associated network. (A) Heatmap of differential proteins in the lipid metabolism–associated network. (B) Expression of COX2, CPT2, ACADVL, and CD36 by Western blots. Values expressed as the mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the MI group; # p < 0.05, ## p < 0.01, and #### p < 0.001 vs. the sham group ( n = 3 for sham, MI, NGR1-L, and NGR1-H groups).

Article Snippet: The primary antibodies against very long-chain specific acyl-CoA dehydrogenase (ACADVL) (Cat# PB1076) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cat# BA2913) were purchased from Boster Biological Technology Co. Ltd. (Wuhan, China).

Techniques: Biomarker Discovery, Expressing, Western Blot