Journal: Oncotarget

Article Title: Middle east respiratory syndrome corona virus spike glycoprotein suppresses macrophage responses via DPP4-mediated induction of IRAK-M and PPARγ

doi: 10.18632/oncotarget.14754

Figure Lengend Snippet: A . Lentiviral particles pseudotyped with MERS S glycoprotein were generated in HEK293T cells following transfection with plasmid pNL4.3R-E-luciferase and pcDNA3.1+ containing the MERS-CoV wild-type or D510A mutant spike glycoprotein. Normalized supernatants were used to infect THP-1 cells and infection was monitored by luciferase assay. The C9-tagged MERS-CoV Spike glycoprotein was detected by Western blot in purified lentiviral particles. HIV-1 p24 envelope protein was used as a normalizing control for the semi-quantitative analysis of MERS-CoV S protein among different types of pseudotyped viruses. THP-1 macrophages were infected with lentiviral particles pseudotyped with MERS wild type (wt) or the D510 mutant S glycoprotein (D510A) of MERS-CoV, or left uninfected (mock), and were stimulated with LPS. TNFα was measured in the supernatants at the naïve state B . and following LPS stimulation C . IL-6 was also measured in naïve and LPS-treated THP1 macrophages D ., E . * p < 0.05, ** p < 0.01

Article Snippet: The MERS-CoV S glycoprotein protein as expressed by the pcDNA3.1+ vector is tagged with C9 tag (TETSQVAPA) and thus the anti-C9 antibody [aE11] (ab90801, Abnova) was used for the detection of the protein.

Techniques: Generated, Transfection, Plasmid Preparation, Luciferase, Mutagenesis, Infection, Western Blot, Purification, Control

Journal: Upsala Journal of Medical Sciences

Article Title: A model to study complement involvement in experimental retinal degeneration

doi: 10.1080/03009734.2018.1431744

Figure Lengend Snippet: Primary antibodies used in immunohistochemical analysis.

Article Snippet: Soluble C5b-9 (sC5b-9) , Mouse monoclonal , Diatec Monoclonals AS, Oslo, Norway , 1:200 , sC5b-9.

Techniques: Immunohistochemical staining, Binding Assay

Journal: Upsala Journal of Medical Sciences

Article Title: A model to study complement involvement in experimental retinal degeneration

doi: 10.1080/03009734.2018.1431744

Figure Lengend Snippet: Immunofluorescent staining for calbindin (green) and the soluble terminal complement complex (sC5b-9, red) in retinal tissue and ARPE cells. Retinal nuclear layers (ONL, INL, and GCL) are indicated. Anti-calbindin antibody labels ganglion, horizontal, and amacrine cells in the retinal tissue (A–E). sC5b-9 (indicated by *) labels blood vessels in non-cultured control retinas (A), in retinas cultured for 3 days (B), and in retinas co-cultured for 3 days (C). After retinal culture or co-culture for 3 or 5 days, ganglion cells are sparsely positive for sC5b-9 (indicated by arrows in B–E). In co-cultured specimens, sC5b-9 seems to coincide with calbindin-positive cells in the INL. Scale bars, 20 μM.

Article Snippet: Soluble C5b-9 (sC5b-9) , Mouse monoclonal , Diatec Monoclonals AS, Oslo, Norway , 1:200 , sC5b-9.

Techniques: Staining, Cell Culture, Control, Co-Culture Assay