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Boster Bio Anti-Cyclin-dependent kinase 2 cdk2 Antibody (Catalog # A00166). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
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Boster Bio Anti-CaMKII Rabbit Monoclonal Antibody catalog # M03241-2. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
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Image Search Results
Journal:
Article Title: The Growth Factor Granulin Interacts with Cyclin T1 and Modulates P-TEFb-Dependent Transcription
doi: 10.1128/MCB.23.5.1688-1702.2003
Figure Lengend Snippet: GST-GrnCDE is phosphorylated by P-TEFb and TAK. (A) GST-GrnCDE is a substrate for CDK9 but not CDK8 or CDK7. CDK9, CDK8, and CDK7 immunocomplexes from 293T WCE were used in kinase assays with purified GST or GST-GrnCDE as the substrate. (B) Depletion of CDK9 from 293T cell WCE. Immunoblotting (IB) detected only trace amounts of CDK9 in depleted (Dep.) WCE (upper panel), whereas the levels of CDK7 and CDK 8 remained the same (middle and lower panels). (C) GST-GrnCDE phosphorylation is greatly reduced after P-TEFb depletion. After binding to GST-Tat48Δ, kinase assays (TAK assays) were conducted with CTD4 peptide (upper panel) or GST-GrnCDE (middle panel) as the substrate. GST-GrnCDE phosphorylation was also examined after binding to GST-GrnCDE (GAK assay; bottom panel).
Article Snippet: Protein A- or protein G-Sepharose beads (10 μl) equilibrated in EBCD buffer were mixed with 1 μg of anti-CDK9, 1 μg of anti-CDK8, or 1 μg of
Techniques: Purification, Western Blot, Binding Assay