Journal: Scientific Reports

Article Title: Effect of non-thermal radiofrequency on body temperature in mice

doi: 10.1038/s41598-020-62789-z

Figure Lengend Snippet: Body temperature on the day 7, after the injection of the TRPM8 antagonist AMG2850 ( a ) and of the vehicle ( b ). The values are expressed as mean ± s.e.m. Arrow indicates the time of AMG2850 injection. Grey areas indicate the period of RF exposure for the EXPO groups. *p < 0.05, two-tailed Mann-Whitney U-tests.

Article Snippet: Thirty minutes prior to the onset of RF exposure in the morning of the seventh day, a TRPM8 channel antagonist, AMG2850 (Alomone Labs, Jerusalem, Israel), was injected intra-peritoneally at a dose of 30 mg.kg −1 in 5 mice of the SHAM group (called SHAM-ANT group) and 6 mice of the EXPO group (called EXPO-ANT group).

Techniques: Injection, Two Tailed Test, MANN-WHITNEY

Journal: Naunyn-Schmiedeberg's Archives of Pharmacology

Article Title: AMG2850, a potent and selective TRPM8 antagonist, is not effective in rat models of inflammatory mechanical hypersensitivity and neuropathic tactile allodynia

doi: 10.1007/s00210-015-1090-9

Figure Lengend Snippet: a AMG2850 potently inhibits both cold temperature- and icilin-induced increase in luminescence believed to reflect intracellular calcium in CHO cells stably expressing rat TRPM8. After a 2-h incubation with coelenterazine, cells were incubated with compounds for 2.5 min prior to the addition of either icilin (1 μM) or cold buffer (12 °C). Luminescence was measured by a CCD camera-based FLASH-luminometer. Concentration-response curves were generated using GraphPad Prism 4.01. Each point in the graphs is an average ± SEM of an experiment conducted in triplicate. Responses of agonists used were normalized to 100 % of maximum. b In a skin-nerve preparation, total percentage of C fibers responding to 300 μM menthol after incubation with vehicle or AMG2850. A response to menthol was defined as three action potentials above baseline over a 2-min interval. Incubation with AMG2850 significantly decreased the percentage of C fibers responding to menthol as compared to vehicle controls (** p < 0.005). c In a skin-nerve preparation, AMG2850 has no effect on C fiber firing in response to sustained mechanical force of increasing intensities. Note an increasing number of action potentials with increased force that is not altered by preincubation of AMG2850

Article Snippet: AMG2850, which was synthesized at Amgen Inc (Thousand Oaks, CA), resulted from an internal medicinal chemistry effort (Horne et al. ).

Techniques: Stable Transfection, Expressing, Incubation, Concentration Assay, Generated

Journal: Naunyn-Schmiedeberg's Archives of Pharmacology

Article Title: AMG2850, a potent and selective TRPM8 antagonist, is not effective in rat models of inflammatory mechanical hypersensitivity and neuropathic tactile allodynia

doi: 10.1007/s00210-015-1090-9

Figure Lengend Snippet: Summary of AMG2850 properties (where indicated using mean ± standard deviation)

Article Snippet: AMG2850, which was synthesized at Amgen Inc (Thousand Oaks, CA), resulted from an internal medicinal chemistry effort (Horne et al. ).

Techniques: Protein Binding

Journal: Naunyn-Schmiedeberg's Archives of Pharmacology

Article Title: AMG2850, a potent and selective TRPM8 antagonist, is not effective in rat models of inflammatory mechanical hypersensitivity and neuropathic tactile allodynia

doi: 10.1007/s00210-015-1090-9

Figure Lengend Snippet: a Intraperitoneal injection of icilin induces wet-dog shakes (WDS) in a dose-dependent manner. b AMG2850 reduces WDS in a dose- and plasma concentration-dependent manner. c AMG2850 (10 mg/kg, p.o.) significantly and fully blocked the cold pressor effect (*** p < 0.001)

Article Snippet: AMG2850, which was synthesized at Amgen Inc (Thousand Oaks, CA), resulted from an internal medicinal chemistry effort (Horne et al. ).

Techniques: Injection, Clinical Proteomics, Concentration Assay

Journal: Naunyn-Schmiedeberg's Archives of Pharmacology

Article Title: AMG2850, a potent and selective TRPM8 antagonist, is not effective in rat models of inflammatory mechanical hypersensitivity and neuropathic tactile allodynia

doi: 10.1007/s00210-015-1090-9

Figure Lengend Snippet: a AMG2850 did not affect the total distance traveled in reversed light cycle rat open field boxes ( p > 0.05) while the positive control compound chlordiazepoxide (CDP; 5.6 mg/kg) significantly reduced distance traveled (* p < 0.05). b Unilateral hindpaw injection of complete Freund’s adjuvant (CFA) causes a reduction in rearing behavior. The positive control indomethacin (indo; 1 mg/kg) significantly ( ++++ p < 0.0001) reversed the CFA-induced reduction in rearing behavior. AMG2850 (100 mg/kg) produced no significant effect (* p > 0.05). c SNL produces a reduction in tactile threshold from approximately 15 g to approximately 5 g. The positive control, gabapentin (200 mg/kg), significantly reversed the reduction in tactile threshold (** p < 0.01). AMG2850 (100 mg/kg) produced no significant effect at 2.5 h post treatment ( p > 0.05)

Article Snippet: AMG2850, which was synthesized at Amgen Inc (Thousand Oaks, CA), resulted from an internal medicinal chemistry effort (Horne et al. ).

Techniques: Positive Control, Injection, Adjuvant, Produced