Journal: Journal of the American Chemical Society

Article Title: Fully Automated Characterization of Protein–Peptide Binding by Microfluidic 2D NMR

doi: 10.1021/jacs.2c13052

Figure Lengend Snippet: Overlay of selected 1 H– 15 N HSQC spectra of hFynSH3, obtained during the automated titration experiment with p85α P91-T104 . The coloring scheme represents the increasing concentration of p85α P91-T104 as indicated on the right. For clarity, only seven 1 H– 15 N HSQC spectra are shown, at specified molar ratios of ligand to protein. The starred Trp* 119 label refers to a signal originating from the tryptophan side chain NHε.

Article Snippet: The p85α P91-T104 peptide was obtained as >97% purity lyophilized powder from the supplier (ChinaPeptides, CN).

Techniques: Titration, Concentration Assay

Journal: Journal of the American Chemical Society

Article Title: Fully Automated Characterization of Protein–Peptide Binding by Microfluidic 2D NMR

doi: 10.1021/jacs.2c13052

Figure Lengend Snippet: Definition of the binding site for p85α P91-T104 on hFynSH3. In (A) a histogram of maximum chemical shift perturbations, Δδ max , is plotted as a function of the amino acid sequence of hFynSH3. Trp* labels refer to the Δδ max reported by the side chain NHε of tryptophan residues, and the horizontal line represents the significance threshold, σ c . In (B) and (C) Δδ max values are mapped on the backbone structure of hFynSH3 (PDB entry: 1AZG ). Colored in brown are the Δδ max values above σ c , including the tryptophan NHε shown as spheres, in beige are the Δδ max below σ c , and unassigned amides are shown in light gray. The p85α P91-T104 peptide is shown in ball and stick representation using the standard atom color coding.

Article Snippet: The p85α P91-T104 peptide was obtained as >97% purity lyophilized powder from the supplier (ChinaPeptides, CN).

Techniques: Binding Assay, Sequencing

Journal: 3 Biotech

Article Title: Agave atrovirens fibers as substrate and support for solid-state fermentation for cellulase production by Trichoderma asperellum

doi: 10.1007/s13205-016-0426-6

Figure Lengend Snippet: Growth analysis of Trichoderma asperellum on Agave atrovirens fibers with a hydrothermal pretreatment

Article Snippet: A set of 4 strains of Trichoderma identified as Trichoderma harzianum (T1-04), Trichoderma asperellum (T2-31) and 2 different more only known as Trichoderma spp. codified as Trichoderma T2 and Trichoderma T2-11 were obtained from the micoteca of the Autonomous Agrarian University Antonio Narro, and the T2 were obtained from the micoteca of the Autonomous University of Coahuila, both in Saltillo Coahuila, Mexico.

Techniques:

Journal: 3 Biotech

Article Title: Agave atrovirens fibers as substrate and support for solid-state fermentation for cellulase production by Trichoderma asperellum

doi: 10.1007/s13205-016-0426-6

Figure Lengend Snippet: Total sugars during fermentation with Trichoderma asperellum

Article Snippet: A set of 4 strains of Trichoderma identified as Trichoderma harzianum (T1-04), Trichoderma asperellum (T2-31) and 2 different more only known as Trichoderma spp. codified as Trichoderma T2 and Trichoderma T2-11 were obtained from the micoteca of the Autonomous Agrarian University Antonio Narro, and the T2 were obtained from the micoteca of the Autonomous University of Coahuila, both in Saltillo Coahuila, Mexico.

Techniques:

Journal: 3 Biotech

Article Title: Agave atrovirens fibers as substrate and support for solid-state fermentation for cellulase production by Trichoderma asperellum

doi: 10.1007/s13205-016-0426-6

Figure Lengend Snippet: Protein content in the fermentation with Trichoderma asperellum

Article Snippet: A set of 4 strains of Trichoderma identified as Trichoderma harzianum (T1-04), Trichoderma asperellum (T2-31) and 2 different more only known as Trichoderma spp. codified as Trichoderma T2 and Trichoderma T2-11 were obtained from the micoteca of the Autonomous Agrarian University Antonio Narro, and the T2 were obtained from the micoteca of the Autonomous University of Coahuila, both in Saltillo Coahuila, Mexico.

Techniques:

Journal: 3 Biotech

Article Title: Agave atrovirens fibers as substrate and support for solid-state fermentation for cellulase production by Trichoderma asperellum

doi: 10.1007/s13205-016-0426-6

Figure Lengend Snippet: Comparison of cellulase production by Trichoderma asperellum with other fungi under SSF

Article Snippet: A set of 4 strains of Trichoderma identified as Trichoderma harzianum (T1-04), Trichoderma asperellum (T2-31) and 2 different more only known as Trichoderma spp. codified as Trichoderma T2 and Trichoderma T2-11 were obtained from the micoteca of the Autonomous Agrarian University Antonio Narro, and the T2 were obtained from the micoteca of the Autonomous University of Coahuila, both in Saltillo Coahuila, Mexico.

Techniques: Comparison, Activity Assay