Journal: Scientific Reports

Article Title: TSC complex decrease the expression of mTOR by regulated miR-199b-3p

doi: 10.1038/s41598-025-85706-8

Figure Lengend Snippet: miR-199b-3p regulate the expression of mTOR ( A ) Quantitative analysis of mTOR in fibroblasts using qRT-PCR. ( B ) Quantitative analysis of mTOR in fibroblasts using western blot and the statistics. The originals gels can be seen in the supplementary figure S5. ( C ) Predicted miR-199b-3p target sequence of the mTOR 3’UTR using miRBase. ( D ) Quantification of miR-199b-3p and mRNA using qRT-PCR, the WT represents the mixture of the equal amount total RNA from the two healthy fibroblasts. Relative expression levels were normalized with GAPDH. ( E ). Luciferase reporter assay. ( F ) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics (low panel) and the statistics (up panel). The originals gels can be seen in the supplementary figure S5. ( G ) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics,β-actin served as a control of total protein.( H ) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR-199b-3p (low panel) and the statistics (up panel). ( I ) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR-199b-3p, β-actin served as a control of total protein. The originals gels can be seen in the supplementary figure S5.

Article Snippet: HEK293T cells were maintained in DMEM (Boster) supplemented with 10% FBS (BI).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Sequencing, Luciferase, Reporter Assay, Control

Journal: Scientific Reports

Article Title: TSC complex decrease the expression of mTOR by regulated miR-199b-3p

doi: 10.1038/s41598-025-85706-8

Figure Lengend Snippet: TSC complex regulates the endogenous content of miR199b-3p, * p < 0.05, ** p < 0.01,*** p < 0.001 comparison with WT. ( A ) The efficiency of knockdown TSC1 in shTSC1-HEK293T cell line. The originals gels can be seen in the supplementary figure S6. ( B ) The expression of miR-199b-3p in shTSC1-HEK293T cells and overexpression TSC1-WT or TSC1-N837fs in HEK293T cell line. ( C ) Quantitative analysis of mTOR in shTSC1-HEK293T cell line and overexpression TSC1-WT or TSC1 N837fs in HEK293T cell line using western blot (low panel) and the statistics (up panel). The originals gels can be seen in the supplementary figure S6. (D) The efficiency of knockdown TSC2 in shTSC2-HEK293T cell line. The originals gels can be seen in the supplementary figure S6. ( E ) The expression of miR-199b-3p in shTSC2-HEK293T cell line and overexpression TSC2-WT or TSC2- Q371fs in HEK293T cell line. ( F ) Quantitative analysis of mTOR in shTSC2-HEK293T cell line (low panel) and overexpression TSC2-WT or TSC2- Q371fs in HEK293T cell line using Western blot (low panel) and the statistics analysis (up panel). The originals gels can be seen in the supplementary figure S6.

Article Snippet: HEK293T cells were maintained in DMEM (Boster) supplemented with 10% FBS (BI).

Techniques: Comparison, Knockdown, Expressing, Over Expression, Western Blot

Journal: iScience

Article Title: Bat RNA viruses employ viral RHIMs orchestrating species-specific cell death programs linked to Z-RNA sensing and ZBP1-RIPK3 signaling

doi: 10.1016/j.isci.2024.111444

Figure Lengend Snippet:

Article Snippet: BSA , GBiosciences , RC1021.

Techniques: Virus, Recombinant, Transfection, Staining, Western Blot, Electron Microscopy, Plasmid Preparation, Software, Microscopy