R33949 Search Results


anti-fca (fca(n) antibody) r3399-1  (Abiocode Inc)
90
Abiocode Inc anti-fca (fca(n) antibody) r3399-1
a , b The degradation of SSF414D was slower than that of SSF414N in the cell-free protein degradation assay. Recombinant purified His-SSF414N/414D was incubated in equal amounts of total proteins extracted from 14-day-old wild-type Col-0 seedlings in the presence of ATP. His-SSF414N/414D was detected with an anti-His antibody. Representative images are shown in ( a ), and relative protein levels are shown in ( b ). In ( b ), data are presented as mean ± SEM, and n = 10 independent replicates. c , d Stability of SSF414N/414D-GFP in vivo. Fourteen-day-old SSF414N-GFP and SSF414D-GFP transgenic seedlings were treated with 50 μM MG132. In ( c , d ), SSF-GFP- and DAPI-stained nuclear signals in roots of 18 independent lines were collected by confocal microscopy and quantitatively analyzed under the same set of fluorescence intensities and at the same scale. From each line, more than 10 seedlings were assayed. Representative pictures are shown in ( c ), and the relative GFP signal level is normalized to DAPI in ( d ). In ( d ), box plot: lower vertical bar = sample minimum, lower box = lower quartile, middle line = median, upper box = upper quartile, upper vertical bar = sample maximum, single points = outliers. e , f Detection of SSF-GFP in vivo from Col-0, SSF414N-GFP, and SSF414D-GFP plants. Total protein was extracted from whole seedlings of 14-day-old Col-0, SSF414N-GFP, and SSF414D-GFP plants, incubated with GFP-trap to enrich GFP-fused protein, and analyzed by <t>immunoblotting</t> using an anti-GFP antibody. Total protein was used as the input loading control. In ( f ), data are presented as mean ± SEM, and n = 10 independent replicates. These experiments were repeated at least three times, and consistent results were obtained. In ( b , d , f ), asterisks indicate significant differences (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, not significant; two-tailed unpaired t test). Source data are provided as a Source data file.
Anti Fca (Fca(N) Antibody) R3399 1, supplied by Abiocode Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-fca (fca(n) antibody) r3399-1/product/Abiocode Inc
Average 90 stars, based on 1 article reviews
anti-fca (fca(n) antibody) r3399-1 - by Bioz Stars, 2026-04
90/100 stars
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tpd52l1 antibody  (NSJ Bioreagents)
N/A
Additional name(s) for this target protein: Tumor protein D52-like 1
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ro1138452  (Aladdin Scientific Corporation)
N/A
RO1138452 is one of the more potent high-affinity ligands and functional antagonists for the human IP (prostacyclin) receptor. RO1138452 antagonizes the carbaprostacyclin-induced activation of human neuroblastoma adenylate cyclase, blocking cyclic AMP accumulation in a dose-dependent
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rac cis-loxoprofen alcohol  (Aladdin Scientific Corporation)
N/A
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rac-metalaxyl carboxylic acid  (Aladdin Scientific Corporation)
N/A
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btbd7 antibody  (NSJ Bioreagents)
N/A
Additional name(s) for this target protein: BTB/POZ domain-containing protein 7
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nos1 antibody  (NSJ Bioreagents)
N/A
Additional name(s) for this target protein: Nitric oxide synthase 1
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rac 4-bromo phenylephrine hydrochloride  (Aladdin Scientific Corporation)
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precursor mirna expression clone for mouse mmu-mir-30e  (Genecopoeia)
N/A
Hairpin precursor miRNA of approximately 150 nucleotides is cloned into lentiviral or non-viral vectors for delivery in virtually all cell types.
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precursor mirna expression clone for mouse mmu-mir-294  (Genecopoeia)
N/A
Hairpin precursor miRNA of approximately 150 nucleotides is cloned into lentiviral or non-viral vectors for delivery in virtually all cell types.
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tom1l1 antibody  (NSJ Bioreagents)
N/A
Additional name(s) for this target protein: Target of myb1-like 1; SRCASM
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hippi antibody  (NSJ Bioreagents)
N/A
Additional name(s) for this target protein: HIP1 protein interactor; intraflagellar transport 57; IFT57
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Image Search Results


a , b The degradation of SSF414D was slower than that of SSF414N in the cell-free protein degradation assay. Recombinant purified His-SSF414N/414D was incubated in equal amounts of total proteins extracted from 14-day-old wild-type Col-0 seedlings in the presence of ATP. His-SSF414N/414D was detected with an anti-His antibody. Representative images are shown in ( a ), and relative protein levels are shown in ( b ). In ( b ), data are presented as mean ± SEM, and n = 10 independent replicates. c , d Stability of SSF414N/414D-GFP in vivo. Fourteen-day-old SSF414N-GFP and SSF414D-GFP transgenic seedlings were treated with 50 μM MG132. In ( c , d ), SSF-GFP- and DAPI-stained nuclear signals in roots of 18 independent lines were collected by confocal microscopy and quantitatively analyzed under the same set of fluorescence intensities and at the same scale. From each line, more than 10 seedlings were assayed. Representative pictures are shown in ( c ), and the relative GFP signal level is normalized to DAPI in ( d ). In ( d ), box plot: lower vertical bar = sample minimum, lower box = lower quartile, middle line = median, upper box = upper quartile, upper vertical bar = sample maximum, single points = outliers. e , f Detection of SSF-GFP in vivo from Col-0, SSF414N-GFP, and SSF414D-GFP plants. Total protein was extracted from whole seedlings of 14-day-old Col-0, SSF414N-GFP, and SSF414D-GFP plants, incubated with GFP-trap to enrich GFP-fused protein, and analyzed by immunoblotting using an anti-GFP antibody. Total protein was used as the input loading control. In ( f ), data are presented as mean ± SEM, and n = 10 independent replicates. These experiments were repeated at least three times, and consistent results were obtained. In ( b , d , f ), asterisks indicate significant differences (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, not significant; two-tailed unpaired t test). Source data are provided as a Source data file.

Journal: Nature Communications

Article Title: Molecular variation in a functionally divergent homolog of FCA regulates flowering time in Arabidopsis thaliana

doi: 10.1038/s41467-020-19666-0

Figure Lengend Snippet: a , b The degradation of SSF414D was slower than that of SSF414N in the cell-free protein degradation assay. Recombinant purified His-SSF414N/414D was incubated in equal amounts of total proteins extracted from 14-day-old wild-type Col-0 seedlings in the presence of ATP. His-SSF414N/414D was detected with an anti-His antibody. Representative images are shown in ( a ), and relative protein levels are shown in ( b ). In ( b ), data are presented as mean ± SEM, and n = 10 independent replicates. c , d Stability of SSF414N/414D-GFP in vivo. Fourteen-day-old SSF414N-GFP and SSF414D-GFP transgenic seedlings were treated with 50 μM MG132. In ( c , d ), SSF-GFP- and DAPI-stained nuclear signals in roots of 18 independent lines were collected by confocal microscopy and quantitatively analyzed under the same set of fluorescence intensities and at the same scale. From each line, more than 10 seedlings were assayed. Representative pictures are shown in ( c ), and the relative GFP signal level is normalized to DAPI in ( d ). In ( d ), box plot: lower vertical bar = sample minimum, lower box = lower quartile, middle line = median, upper box = upper quartile, upper vertical bar = sample maximum, single points = outliers. e , f Detection of SSF-GFP in vivo from Col-0, SSF414N-GFP, and SSF414D-GFP plants. Total protein was extracted from whole seedlings of 14-day-old Col-0, SSF414N-GFP, and SSF414D-GFP plants, incubated with GFP-trap to enrich GFP-fused protein, and analyzed by immunoblotting using an anti-GFP antibody. Total protein was used as the input loading control. In ( f ), data are presented as mean ± SEM, and n = 10 independent replicates. These experiments were repeated at least three times, and consistent results were obtained. In ( b , d , f ), asterisks indicate significant differences (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, not significant; two-tailed unpaired t test). Source data are provided as a Source data file.

Article Snippet: SDS-PAGE was used to separate the proteins, and the target proteins were detected by immunoblotting with Anti-FCA (FCA(N) antibody) (Abiocode, R3399-1, dilution: 1:2000), or Anti-Ubiquitin antibody [Ubi-1] (Abcam, ab7254, dilution: 1:2000) antibodies.

Techniques: Degradation Assay, Recombinant, Purification, Incubation, In Vivo, Transgenic Assay, Staining, Confocal Microscopy, Fluorescence, Western Blot, Control, Two Tailed Test