Journal: Biomolecules

Article Title: Human Alpha-1 Antitrypsin Attenuates ENaC and MARCKS and Lowers Blood Pressure in Hypertensive Diabetic db/db Mice

doi: 10.3390/biom13010066

Figure Lengend Snippet: Western blot and densitometric analysis of cathepsin B and kidney kallikrein protein expression after hAAT or vehicle treatment. ( A ). Western blot for cathepsin B protein expression. Eighty micrograms of total protein from kidney cortex lysates from hypertensive diabetic db/db mice treated with either hAAT or vehicle. ( B ). Densitometry values were obtained using Image J for the cathepsin B band in panel A normalized to the Ponceau staining. N = 6 mice in each group. ( C ). Western blot for kallikrein protein expression. Eighty micrograms of total protein from kidney cortex lysates from hypertensive diabetic db/db mice treated with either hAAT or vehicle. ( D ). Densitometry values were obtained using Image J for the kallikrein band in panel C normalized to the Ponceau staining. N = 6 mice in each group.

Article Snippet: The membranes were washed twice with 1×TBS solution, and then incubated for 8–12 h in primary antibodies (ENaC alpha 59 [ ]), MARCKS (abcam, ab72459), aquaporin-2 (abcam, ab230170), NPRC [ ], cathepsin B (Cell Signaling Tech, 3383, Danvers, MA, USA), Kallikrein (Boster Bio, PA2038, Pleasanton, CA, USA), caveolin-1 (Cell Signaling Tech, ab2910), annexin A2 (Cell Signaling Tech, 8235), GAPDH-HRP (Cell Signaling Tech, 3683), and TSG101 (abcam, ab30871) at 4 degrees Celsius.

Techniques: Western Blot, Expressing, Staining