Journal: Cancer Research

Article Title: Cardiac Dysfunction Promotes Cancer Progression via Multiple Secreted Factors

doi: 10.1158/0008-5472.CAN-21-2463

Figure Lengend Snippet: Tumor progression phenotype is mediated by multiple secreted factors derived from the heart, tumor, and other tissues. A, Serum from either ATF3-transgenic or control mice was used to probe proteome cytokine array. For each protein, serum levels are presented as fold change relative to control serum. B, Serum levels as in A , obtained by ELISA for ceruloplasmin (CP) and CTGF and fibronectin (FN). C and D, Heart ( C ) and tumor ( D ) mRNA levels of the indicated genes were measured by qRT-PCR. Color code for the secreted factors from the heart (red), tumor (black), tumor and heart simultaneously (light blue), and other tissues and organs (green). Data are presented as mean ±SD relative expression compared with control, which was determined as 1. One-way ANOVA followed by Tukey posttests or multiple Student t tests. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Article Snippet: Quantification of candidate secreted factors protein levels in the blood was performed with Mouse CTGF-Connective Tissue Growth Factor ELISA Kit (E-EL-M0340, Elabscience), Mouse Ceruloplasmin ELISA Kit (NBP2–82160, Elabscience, Novus Biologicals) and mouse Fibronectin ELISA Kit (E-EL-M0506, Elabscience) according to the manufacturer's instructions.

Techniques: Derivative Assay, Transgenic Assay, Control, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing