We use essential cookies to operate our site. With your consent, we may also use non-essential cookies to improve your user experience and to analyze website traffic. Click "Accept" to accept cookies and go directly to the site, click "Reject" to reject all but cookies strictly necessary for the functioning of this site. You can reset your cookie settings at any time by visiting your Bioz "my account" page and selecting the "reset cookie preferences" link.

Accept Reject

     Combing through more than 330 million products and articles...      Welcome to Your Next Discovery   
All (3)
New England Biolabs
MilliporeSigma
Fisher Scientific
 

About 3 products / 474 snippets / 9 images

96 / 100
Bioz Stars
Bst DNA Polymerase, Large Fragment
New England Biolabs  
Catalog#: M0275
Techniques
 
Article Snippets
"es (San Diego, CA). E. coli total RNA was purchased from Applied Biosystems (Austin, TX). The following commercially available DNA polymerases were purchased: DNA polymerase I large (Klenow) fragment (New England Biolabs, Ipswich, MA), 3′→5′ exo − Klenow fragment (NEB), Bst DNA polymerase large fragment (NEB), Therminator ™ DNA polymerase (NEB), and Taq DNA polymerase (Promega). O’GeneRuler ™ ultra"  (see more)
96 / 100
Bioz Stars
Bst DNA Polymerase, Large Fragment
New England Biolabs  
star_border
Catalog#: M0275
Techniques
 
Article Snippets
"es (San Diego, CA). E. coli total RNA was purchased from Applied Biosystems (Austin, TX). The following commercially available DNA polymerases were purchased: DNA polymerase I large (Klenow) fragment (New England Biolabs, Ipswich, MA), 3′→5′ exo − Klenow fragment (NEB), Bst DNA polymerase large fragment (NEB), Therminator ™ DNA polymerase (NEB), and Taq DNA polymerase (Promega). O’GeneRuler ™ ultra"  (see more)
91 / 100
Bioz Stars
M0275
MilliporeSigma / Sigma Aldrich  
star_border
Techniques
 
Article Snippets
"M0275) were from Sigma Aldrich (Taufkirchen, Germany)."  (see more)
5+  more images
Restriction analysis and Southern blot hybridization of the amplified M13mp18 <t>DNA.</t> (A) Electrophoretic analysis of the LAMP amplified M13mp18 product. Six hundred copies of M13mp18 DNA were amplified by LAMP with the specific primers designed on the sequences shown in Figure 2 and run on a 2% agarose gel followed by SYBR Green I staining. Lane M, 100 bp ladder used as size marker (New England Biolabs); lane 1, M13mpl8 DNA digested with PvuII; lane 2, LAMP without <t>Bst</t> <t>DNA</t> <t>polymerase;</t> lane 3, LAMP without target M13 DNA; lane 4, complete LAMP; lanes 5–7, complete LAMP products after digestion with BamHI, PstI and PvuII, respectively (one fifth of the digests were loaded). (B–D) Southern blot analysis of the LAMP products. The 2% agarose gel shown in (A) was used for Southern blot hybridization with M13-281 DNA (B), M13-333 DNA (C) and M13BIP (D) as probes. (E) Alkaline agarose gel electrophoresis of the LAMP products. Lane m, λ DNA HindIII digests; lane 4, the same sample as in (A).
SB
HYBRID
AMP
GE-A
SYBR-G
Schematic of RCA for SNP identification. The allele-specific probes hybridise to target <t>DNA</t> so that the 3′-terminal nucleotide is at the SNP position. Ligation and therefore circularisation of the probes only occurs if the 3′-terminal nucleotide is complementary to the SNP site. If there is a mismatch at the 3′ base, complete hybridisation is prevented and ligation of the probe occurs at a much reduced rate. Successfully circularised probes are detected by amplification in the presence of amplification primers. The first primer hybridises to its complementary region on the probe backbone. The primer is extended as continued polymerisation and displacement result in the generation of a single-stranded concatamer of the original probe. The second primer binds to each tandem repeat of the original probe which results in strand displacement and branching of the RCA product. The second RCA primer contains a 5′-hairpin loop that is labelled with a fluorophore and quencher. When the hairpin stem is displaced by <t>DNA</t> <t>polymerase</t> during extension, the fluorophore and quencher are separated and a fluorescent signal is emitted.
LIGATION
HYBRID
AMP
a , One-pot WS-CRISPR reaction mixture is first prepared in one tube. After distributed into QuantStudio 3D digital chip, over ten thousand sub-nanoliter (∼0.7 nL) microreactions are isolated in microwells. When incubated at 52°C, each microreaction with SARS-CoV-2 RNA target undertakes WS-CRISPR reaction and generates strong green fluorescence (positive spots), whereas not in those without target (negative spots). Scale bar is 300 μm. Through detecting and counting the positive microreactions (or spots), SARS-CoV-2 RNA can be quantified based on the proportion of positive spots. b , Working principle of one-pot WS-CRISPR assay for SARS-CoV-2 detection. The WS-CRISPR reaction mixture contains Cas12a-crRNA complex, six DAMP primers (two outer primers of FO and RO, two inner primers of FI and RI, and two competition primers of FC and RC), ssDNA-FQ reporter, SuperScript IV reverse transcriptase, <t>Bst</t> <t>DNA</t> polymerase, and SARS-CoV-2 RNA in a one-pot format.
CRISPR
ISOLAT
INCU
FLUOR
Schematic representation of the RAM assay. Target <t>DNA,</t> capture probe, C-probe, and paramagnetic bead are added to hybridization buffer to allow formation of a hybrid complex. The hybrid is captured on a paramagnetic bead, allowing extensive washing to remove unbound C-probe and cellular components. The C-probe aligned on the target is linked together by a DNA ligase. RAM amplification is then carried out by the addition of forward (▸) and reverse (◃) primers and <t>DNA</t> <t>polymerase</t> (shaded ovals). The forward primer bound to the C-probe is extended by the polymerase and continues after one round of synthesis by displacing the bound forward primer and its extended product, generating a long ssDNA with repeated sequence. With the reactions going on, multiple reverse primers can bind to the nascent ssDNA as their binding sites are available. Each bound reverse primer will be extended and displace the downstream primers and their extended products. The forward primer binding sites of the displaced ssDNA are then available for the forward primer to bind and extend similarly, thus forming a large ramifying DNA complex. Finally, the RAM products are examined by gel electrophoresis after EcoRI digestion. Insert: the C-probe hybridizes to the target through their complementary regions (f and g), and sequence at the noncomplementary region (e) is generic for the binding of primers. The C-probe-target hybrid is captured on a paramagnetic bead (a) through the binding of the biotin moiety (c) of the capture probe (d) to streptavidin (b) coated on the beads.
HYBRID
AMP
SEQ
BIND-A
NAE
×
80 / 100
Bioz Stars
M0275 Crystal violet Fisher Scientific Cat#C581-100
Fisher Scientific  
star_border
Techniques
 
Article Snippets
".. Corning Cat# 25-051-Cl Minimum Essential Medium Eagle (MEM) 10X Sigma Cat# M0275 Crystal violet Fisher Scientific Cat#C581-100 Methyl cellulose Sigma Cat#M0512 Protease RNAscope Fluorescent Multiplex Reagent Kit Cat .."  (see more)