GPC-100 Search Results


gpc mono gpc-100  (Sepax Inc)
90
Sepax Inc gpc mono gpc-100
Gpc Mono Gpc 100, supplied by Sepax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gpc mono gpc-100/product/Sepax Inc
Average 90 stars, based on 1 article reviews
gpc mono gpc-100 - by Bioz Stars, 2026-04
90/100 stars
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synchropak gpc-100  (SynChrom Inc)
90
SynChrom Inc synchropak gpc-100
Synchropak Gpc 100, supplied by SynChrom Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/synchropak gpc-100/product/SynChrom Inc
Average 90 stars, based on 1 article reviews
synchropak gpc-100 - by Bioz Stars, 2026-04
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gpc100 column  (Eichrom Technologies LLC)
90
Eichrom Technologies LLC gpc100 column
Gpc100 Column, supplied by Eichrom Technologies LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gpc100 column/product/Eichrom Technologies LLC
Average 90 stars, based on 1 article reviews
gpc100 column - by Bioz Stars, 2026-04
90/100 stars
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synchropack gpc 100 hplc (ng108)  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd synchropack gpc 100 hplc (ng108)
Cofractionation of endogenous RGS proteins and COPI. (A) Endogenous RGS4 cofractionates with β-COP in <t>NG108</t> cells by gel filtration. Protein molecular weight standards were loaded onto a Synchropack <t>GPC</t> <t>100</t> HPLC column; the elution profile is shown in the chromatograph for thyroglobulin (669,000), aldolase (150,000), and chymotrypsinogen (25,000) molecular weight standards (solid line). NG108 cytosol was loaded onto the column, and 0.5-ml fractions were collected. The entire amount of protein in peak fractions was separated on 12% gels, transferred to nitrocellulose, and probed with specific antibodies against β-COP or RGS4 as indicated. (B) Characterization of RGS2 antibody. 6His-RGS2 or -RGS4 (50 ng) was electrophoresed and immunoblotted with RGS2 antiserum. The antiserum was preincubated with either a control peptide (FLAG; left panel) or immunizing peptide (middle panel) (both at 10 μg/ml) for 30 min at room temperature before immunodetection. Right panel shows lysate from HEK293T cells (100–150 μg of protein) treated with PMA (100 ng/ml for 2 h) (lane 1) or 6His-RGS2 as a positive control (lane 2) electrophoresed and blotted with RGS2 antiserum. (C) Endogenous RGS2 cofractionates with β′-COP in HEK293T cells. The cytosolic fraction from PMA-treated HEK293T cells was loaded onto a Superdex 200 FPLC column and fractionated as in A. Molecular weight marker migrations are indicated by arrows (thyroglobulin, 669,000; γ-globulin, 158,000; and ovalbumin, 44,000). Peak protein-containing fractions were separated by SDS-PAGE and immunodetected with antibodies for β′-COP (top panel) or RGS2 (bottom panel).
Synchropack Gpc 100 Hplc (Ng108), supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/synchropack gpc 100 hplc (ng108)/product/Amersham Pharmacia Biotech Ltd
Average 90 stars, based on 1 article reviews
synchropack gpc 100 hplc (ng108) - by Bioz Stars, 2026-04
90/100 stars
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gel filtration chromatography gpc 100  (Eprogen Inc)
90
Eprogen Inc gel filtration chromatography gpc 100
Cofractionation of endogenous RGS proteins and COPI. (A) Endogenous RGS4 cofractionates with β-COP in <t>NG108</t> cells by gel filtration. Protein molecular weight standards were loaded onto a Synchropack <t>GPC</t> <t>100</t> HPLC column; the elution profile is shown in the chromatograph for thyroglobulin (669,000), aldolase (150,000), and chymotrypsinogen (25,000) molecular weight standards (solid line). NG108 cytosol was loaded onto the column, and 0.5-ml fractions were collected. The entire amount of protein in peak fractions was separated on 12% gels, transferred to nitrocellulose, and probed with specific antibodies against β-COP or RGS4 as indicated. (B) Characterization of RGS2 antibody. 6His-RGS2 or -RGS4 (50 ng) was electrophoresed and immunoblotted with RGS2 antiserum. The antiserum was preincubated with either a control peptide (FLAG; left panel) or immunizing peptide (middle panel) (both at 10 μg/ml) for 30 min at room temperature before immunodetection. Right panel shows lysate from HEK293T cells (100–150 μg of protein) treated with PMA (100 ng/ml for 2 h) (lane 1) or 6His-RGS2 as a positive control (lane 2) electrophoresed and blotted with RGS2 antiserum. (C) Endogenous RGS2 cofractionates with β′-COP in HEK293T cells. The cytosolic fraction from PMA-treated HEK293T cells was loaded onto a Superdex 200 FPLC column and fractionated as in A. Molecular weight marker migrations are indicated by arrows (thyroglobulin, 669,000; γ-globulin, 158,000; and ovalbumin, 44,000). Peak protein-containing fractions were separated by SDS-PAGE and immunodetected with antibodies for β′-COP (top panel) or RGS2 (bottom panel).
Gel Filtration Chromatography Gpc 100, supplied by Eprogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gel filtration chromatography gpc 100/product/Eprogen Inc
Average 90 stars, based on 1 article reviews
gel filtration chromatography gpc 100 - by Bioz Stars, 2026-04
90/100 stars
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synchropack-gpc100 column  (Varian Medical)
90
Varian Medical synchropack-gpc100 column
Cofractionation of endogenous RGS proteins and COPI. (A) Endogenous RGS4 cofractionates with β-COP in <t>NG108</t> cells by gel filtration. Protein molecular weight standards were loaded onto a Synchropack <t>GPC</t> <t>100</t> HPLC column; the elution profile is shown in the chromatograph for thyroglobulin (669,000), aldolase (150,000), and chymotrypsinogen (25,000) molecular weight standards (solid line). NG108 cytosol was loaded onto the column, and 0.5-ml fractions were collected. The entire amount of protein in peak fractions was separated on 12% gels, transferred to nitrocellulose, and probed with specific antibodies against β-COP or RGS4 as indicated. (B) Characterization of RGS2 antibody. 6His-RGS2 or -RGS4 (50 ng) was electrophoresed and immunoblotted with RGS2 antiserum. The antiserum was preincubated with either a control peptide (FLAG; left panel) or immunizing peptide (middle panel) (both at 10 μg/ml) for 30 min at room temperature before immunodetection. Right panel shows lysate from HEK293T cells (100–150 μg of protein) treated with PMA (100 ng/ml for 2 h) (lane 1) or 6His-RGS2 as a positive control (lane 2) electrophoresed and blotted with RGS2 antiserum. (C) Endogenous RGS2 cofractionates with β′-COP in HEK293T cells. The cytosolic fraction from PMA-treated HEK293T cells was loaded onto a Superdex 200 FPLC column and fractionated as in A. Molecular weight marker migrations are indicated by arrows (thyroglobulin, 669,000; γ-globulin, 158,000; and ovalbumin, 44,000). Peak protein-containing fractions were separated by SDS-PAGE and immunodetected with antibodies for β′-COP (top panel) or RGS2 (bottom panel).
Synchropack Gpc100 Column, supplied by Varian Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/synchropack-gpc100 column/product/Varian Medical
Average 90 stars, based on 1 article reviews
synchropack-gpc100 column - by Bioz Stars, 2026-04
90/100 stars
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column nucleogel gpc 100-5, ×  (MACHEREY NAGEL)
90
MACHEREY NAGEL column nucleogel gpc 100-5, ×
Cofractionation of endogenous RGS proteins and COPI. (A) Endogenous RGS4 cofractionates with β-COP in <t>NG108</t> cells by gel filtration. Protein molecular weight standards were loaded onto a Synchropack <t>GPC</t> <t>100</t> HPLC column; the elution profile is shown in the chromatograph for thyroglobulin (669,000), aldolase (150,000), and chymotrypsinogen (25,000) molecular weight standards (solid line). NG108 cytosol was loaded onto the column, and 0.5-ml fractions were collected. The entire amount of protein in peak fractions was separated on 12% gels, transferred to nitrocellulose, and probed with specific antibodies against β-COP or RGS4 as indicated. (B) Characterization of RGS2 antibody. 6His-RGS2 or -RGS4 (50 ng) was electrophoresed and immunoblotted with RGS2 antiserum. The antiserum was preincubated with either a control peptide (FLAG; left panel) or immunizing peptide (middle panel) (both at 10 μg/ml) for 30 min at room temperature before immunodetection. Right panel shows lysate from HEK293T cells (100–150 μg of protein) treated with PMA (100 ng/ml for 2 h) (lane 1) or 6His-RGS2 as a positive control (lane 2) electrophoresed and blotted with RGS2 antiserum. (C) Endogenous RGS2 cofractionates with β′-COP in HEK293T cells. The cytosolic fraction from PMA-treated HEK293T cells was loaded onto a Superdex 200 FPLC column and fractionated as in A. Molecular weight marker migrations are indicated by arrows (thyroglobulin, 669,000; γ-globulin, 158,000; and ovalbumin, 44,000). Peak protein-containing fractions were separated by SDS-PAGE and immunodetected with antibodies for β′-COP (top panel) or RGS2 (bottom panel).
Column Nucleogel Gpc 100 5, ×, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/column nucleogel gpc 100-5, ×/product/MACHEREY NAGEL
Average 90 stars, based on 1 article reviews
column nucleogel gpc 100-5, × - by Bioz Stars, 2026-04
90/100 stars
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gel permeation columns macrosphere gpc 100  (Alltech Inc)
90
Alltech Inc gel permeation columns macrosphere gpc 100
Cofractionation of endogenous RGS proteins and COPI. (A) Endogenous RGS4 cofractionates with β-COP in <t>NG108</t> cells by gel filtration. Protein molecular weight standards were loaded onto a Synchropack <t>GPC</t> <t>100</t> HPLC column; the elution profile is shown in the chromatograph for thyroglobulin (669,000), aldolase (150,000), and chymotrypsinogen (25,000) molecular weight standards (solid line). NG108 cytosol was loaded onto the column, and 0.5-ml fractions were collected. The entire amount of protein in peak fractions was separated on 12% gels, transferred to nitrocellulose, and probed with specific antibodies against β-COP or RGS4 as indicated. (B) Characterization of RGS2 antibody. 6His-RGS2 or -RGS4 (50 ng) was electrophoresed and immunoblotted with RGS2 antiserum. The antiserum was preincubated with either a control peptide (FLAG; left panel) or immunizing peptide (middle panel) (both at 10 μg/ml) for 30 min at room temperature before immunodetection. Right panel shows lysate from HEK293T cells (100–150 μg of protein) treated with PMA (100 ng/ml for 2 h) (lane 1) or 6His-RGS2 as a positive control (lane 2) electrophoresed and blotted with RGS2 antiserum. (C) Endogenous RGS2 cofractionates with β′-COP in HEK293T cells. The cytosolic fraction from PMA-treated HEK293T cells was loaded onto a Superdex 200 FPLC column and fractionated as in A. Molecular weight marker migrations are indicated by arrows (thyroglobulin, 669,000; γ-globulin, 158,000; and ovalbumin, 44,000). Peak protein-containing fractions were separated by SDS-PAGE and immunodetected with antibodies for β′-COP (top panel) or RGS2 (bottom panel).
Gel Permeation Columns Macrosphere Gpc 100, supplied by Alltech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gel permeation columns macrosphere gpc 100/product/Alltech Inc
Average 90 stars, based on 1 article reviews
gel permeation columns macrosphere gpc 100 - by Bioz Stars, 2026-04
90/100 stars
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cosmosil-packed column combined with gpc-300 and gpc-100  (Nacalai)
90
Nacalai cosmosil-packed column combined with gpc-300 and gpc-100
Cofractionation of endogenous RGS proteins and COPI. (A) Endogenous RGS4 cofractionates with β-COP in <t>NG108</t> cells by gel filtration. Protein molecular weight standards were loaded onto a Synchropack <t>GPC</t> <t>100</t> HPLC column; the elution profile is shown in the chromatograph for thyroglobulin (669,000), aldolase (150,000), and chymotrypsinogen (25,000) molecular weight standards (solid line). NG108 cytosol was loaded onto the column, and 0.5-ml fractions were collected. The entire amount of protein in peak fractions was separated on 12% gels, transferred to nitrocellulose, and probed with specific antibodies against β-COP or RGS4 as indicated. (B) Characterization of RGS2 antibody. 6His-RGS2 or -RGS4 (50 ng) was electrophoresed and immunoblotted with RGS2 antiserum. The antiserum was preincubated with either a control peptide (FLAG; left panel) or immunizing peptide (middle panel) (both at 10 μg/ml) for 30 min at room temperature before immunodetection. Right panel shows lysate from HEK293T cells (100–150 μg of protein) treated with PMA (100 ng/ml for 2 h) (lane 1) or 6His-RGS2 as a positive control (lane 2) electrophoresed and blotted with RGS2 antiserum. (C) Endogenous RGS2 cofractionates with β′-COP in HEK293T cells. The cytosolic fraction from PMA-treated HEK293T cells was loaded onto a Superdex 200 FPLC column and fractionated as in A. Molecular weight marker migrations are indicated by arrows (thyroglobulin, 669,000; γ-globulin, 158,000; and ovalbumin, 44,000). Peak protein-containing fractions were separated by SDS-PAGE and immunodetected with antibodies for β′-COP (top panel) or RGS2 (bottom panel).
Cosmosil Packed Column Combined With Gpc 300 And Gpc 100, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cosmosil-packed column combined with gpc-300 and gpc-100/product/Nacalai
Average 90 stars, based on 1 article reviews
cosmosil-packed column combined with gpc-300 and gpc-100 - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


Cofractionation of endogenous RGS proteins and COPI. (A) Endogenous RGS4 cofractionates with β-COP in NG108 cells by gel filtration. Protein molecular weight standards were loaded onto a Synchropack GPC 100 HPLC column; the elution profile is shown in the chromatograph for thyroglobulin (669,000), aldolase (150,000), and chymotrypsinogen (25,000) molecular weight standards (solid line). NG108 cytosol was loaded onto the column, and 0.5-ml fractions were collected. The entire amount of protein in peak fractions was separated on 12% gels, transferred to nitrocellulose, and probed with specific antibodies against β-COP or RGS4 as indicated. (B) Characterization of RGS2 antibody. 6His-RGS2 or -RGS4 (50 ng) was electrophoresed and immunoblotted with RGS2 antiserum. The antiserum was preincubated with either a control peptide (FLAG; left panel) or immunizing peptide (middle panel) (both at 10 μg/ml) for 30 min at room temperature before immunodetection. Right panel shows lysate from HEK293T cells (100–150 μg of protein) treated with PMA (100 ng/ml for 2 h) (lane 1) or 6His-RGS2 as a positive control (lane 2) electrophoresed and blotted with RGS2 antiserum. (C) Endogenous RGS2 cofractionates with β′-COP in HEK293T cells. The cytosolic fraction from PMA-treated HEK293T cells was loaded onto a Superdex 200 FPLC column and fractionated as in A. Molecular weight marker migrations are indicated by arrows (thyroglobulin, 669,000; γ-globulin, 158,000; and ovalbumin, 44,000). Peak protein-containing fractions were separated by SDS-PAGE and immunodetected with antibodies for β′-COP (top panel) or RGS2 (bottom panel).

Journal:

Article Title: RGS4 and RGS2 Bind Coatomer and Inhibit COPI Association with Golgi Membranes and Intracellular Transport

doi:

Figure Lengend Snippet: Cofractionation of endogenous RGS proteins and COPI. (A) Endogenous RGS4 cofractionates with β-COP in NG108 cells by gel filtration. Protein molecular weight standards were loaded onto a Synchropack GPC 100 HPLC column; the elution profile is shown in the chromatograph for thyroglobulin (669,000), aldolase (150,000), and chymotrypsinogen (25,000) molecular weight standards (solid line). NG108 cytosol was loaded onto the column, and 0.5-ml fractions were collected. The entire amount of protein in peak fractions was separated on 12% gels, transferred to nitrocellulose, and probed with specific antibodies against β-COP or RGS4 as indicated. (B) Characterization of RGS2 antibody. 6His-RGS2 or -RGS4 (50 ng) was electrophoresed and immunoblotted with RGS2 antiserum. The antiserum was preincubated with either a control peptide (FLAG; left panel) or immunizing peptide (middle panel) (both at 10 μg/ml) for 30 min at room temperature before immunodetection. Right panel shows lysate from HEK293T cells (100–150 μg of protein) treated with PMA (100 ng/ml for 2 h) (lane 1) or 6His-RGS2 as a positive control (lane 2) electrophoresed and blotted with RGS2 antiserum. (C) Endogenous RGS2 cofractionates with β′-COP in HEK293T cells. The cytosolic fraction from PMA-treated HEK293T cells was loaded onto a Superdex 200 FPLC column and fractionated as in A. Molecular weight marker migrations are indicated by arrows (thyroglobulin, 669,000; γ-globulin, 158,000; and ovalbumin, 44,000). Peak protein-containing fractions were separated by SDS-PAGE and immunodetected with antibodies for β′-COP (top panel) or RGS2 (bottom panel).

Article Snippet: The cytosolic fraction (supernatant) was concentrated by ultrafiltration and loaded onto a Synchropack GPC 100 HPLC (NG108) or Superdex 200 fast performance liquid chromatography (FPLC) (HEK293T) column (Amersham Pharmacia Biotech).

Techniques: Filtration, Molecular Weight, Immunodetection, Positive Control, Marker, SDS Page