Journal: Molecular medicine reports

Article Title: Effects of the eukaryotic initiation factor 6 gene on expression levels of inflammatory mediators in M2 macrophages during scar repair.

doi: 10.3892/mmr.2016.5294

Figure Lengend Snippet: Figure 4. mRNA expression levels of TIMP‑2, MMP‑2 and VEGF in M2 macrophages. mRNA levels were determined by reverse transcription‑quan titative polymerase chain reaction analysis, and protein expression levels were determined by ELISA. Data are presented as the mean ± standard deviation. **P<0.05, compared with eIF6+/+ M2 macrophages. eIF6, eukaryotic initia tion factor 6; TIMP, tissue inhibitor of metalloproteinase‑2; MMP‑2, matrix metalloproteinase‑2; VEGF, vascular endothelial growth factor.

Article Snippet: The supernatant obtained from the culture medium of macrophages following stimulation with IL-4 for 24 h was collected, and the expression levels of VEGF (cat. no. EK0506), MMP-2 (cat. no. EK0511) and TIMP-2 (cat. no. EK0322) were detected using ELISA kits (Boster Biological Technology Co., Ltd., Wuhan, China), in strict accordance with the manufacturer's protocol.

Techniques: Expressing, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation