D012 Search Results


versaladder  (Gold Biotechnology Inc)
94
Gold Biotechnology Inc versaladder
a. Schematic of two different 300 bp piRNAi transgenes, each encoding three sg-piRNAs. 300 bp oligos synthesized at large scale on oligo chips are delivered as arrayed sub-pools with 121 unique oligos in each of 384 wells. The piRNA transgenes are flanked by orthogonal forward and reverse primer sites that allow ‘dial-out’ PCR of transgenes from complex oligo pools (Kosuri et al., 2010). Each oligo pool contains 121 different oligos with a maximum length of 300 base pairs (Matrix Oligo Pools, Twist Bioscience). Two piRNAi transgenes (encoding six sg-piRNAs) are required for silencing. Each pool can, therefore, target 60 genes and individual piRNAi transgenes can be ‘dialed out’ using 16 orthogonal primers (8 forward * 8 reverse = 64 unique combinations). Restriction sites (DraI or EcoRV) allow Sanger sequencing of pair-wise amplified piRNA transgenes. b. 96-well amplification of 60 different piRNA transgene pairs targeting him-5 using two rounds of PCR. The first PCR was a bulk amplification of all oligos in the pool using all 16 amplification primers concurrently. 60 specific piRNA transgenes were amplified in a second round of PCR performed with pair-wise orthogonal primers listed above wells (expected size = 300 bp, indicated by arrow). Control reactions contained no template to assess background amplification of contaminants. With no optimization, we were able to amplify 51 of 60 piRNA transgenes (nine wells with weak or no band at 300 bp). Ladder = 100–10,000 bp <t>VersaLadder</t> (GoldBio). For a large-scale library (for example, a whole-genome library) a subcloning step after the first bulk amplification and transformation of the amplified oligo pool into bacteria would maintain long-term integrity and facilitate distribution of the oligo-pool as lyophilized plasmid pools. c. Representative example of Sanger sequencing of a PCR-amplified piRNA transgene from the oligo pool. 12 of 12 sequenced PCR products contained the expected three unique sg-piRNAs. From the sequencing trace, a low level of cross-talk is visible (minor peaks below the three sg-piRNAs), which can likely be minimized by reducing the number of PCR cycles and using a sub-cloned library as a PCR template.
Versaladder, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/versaladder/product/Gold Biotechnology Inc
Average 94 stars, based on 1 article reviews
versaladder - by Bioz Stars, 2026-04
94/100 stars
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maoa inhibitor screening kit e-bc-d012  (Elabscience Biotechnology)
90
Elabscience Biotechnology maoa inhibitor screening kit e-bc-d012
a. Schematic of two different 300 bp piRNAi transgenes, each encoding three sg-piRNAs. 300 bp oligos synthesized at large scale on oligo chips are delivered as arrayed sub-pools with 121 unique oligos in each of 384 wells. The piRNA transgenes are flanked by orthogonal forward and reverse primer sites that allow ‘dial-out’ PCR of transgenes from complex oligo pools (Kosuri et al., 2010). Each oligo pool contains 121 different oligos with a maximum length of 300 base pairs (Matrix Oligo Pools, Twist Bioscience). Two piRNAi transgenes (encoding six sg-piRNAs) are required for silencing. Each pool can, therefore, target 60 genes and individual piRNAi transgenes can be ‘dialed out’ using 16 orthogonal primers (8 forward * 8 reverse = 64 unique combinations). Restriction sites (DraI or EcoRV) allow Sanger sequencing of pair-wise amplified piRNA transgenes. b. 96-well amplification of 60 different piRNA transgene pairs targeting him-5 using two rounds of PCR. The first PCR was a bulk amplification of all oligos in the pool using all 16 amplification primers concurrently. 60 specific piRNA transgenes were amplified in a second round of PCR performed with pair-wise orthogonal primers listed above wells (expected size = 300 bp, indicated by arrow). Control reactions contained no template to assess background amplification of contaminants. With no optimization, we were able to amplify 51 of 60 piRNA transgenes (nine wells with weak or no band at 300 bp). Ladder = 100–10,000 bp <t>VersaLadder</t> (GoldBio). For a large-scale library (for example, a whole-genome library) a subcloning step after the first bulk amplification and transformation of the amplified oligo pool into bacteria would maintain long-term integrity and facilitate distribution of the oligo-pool as lyophilized plasmid pools. c. Representative example of Sanger sequencing of a PCR-amplified piRNA transgene from the oligo pool. 12 of 12 sequenced PCR products contained the expected three unique sg-piRNAs. From the sequencing trace, a low level of cross-talk is visible (minor peaks below the three sg-piRNAs), which can likely be minimized by reducing the number of PCR cycles and using a sub-cloned library as a PCR template.
Maoa Inhibitor Screening Kit E Bc D012, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/maoa inhibitor screening kit e-bc-d012/product/Elabscience Biotechnology
Average 90 stars, based on 1 article reviews
maoa inhibitor screening kit e-bc-d012 - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


a. Schematic of two different 300 bp piRNAi transgenes, each encoding three sg-piRNAs. 300 bp oligos synthesized at large scale on oligo chips are delivered as arrayed sub-pools with 121 unique oligos in each of 384 wells. The piRNA transgenes are flanked by orthogonal forward and reverse primer sites that allow ‘dial-out’ PCR of transgenes from complex oligo pools (Kosuri et al., 2010). Each oligo pool contains 121 different oligos with a maximum length of 300 base pairs (Matrix Oligo Pools, Twist Bioscience). Two piRNAi transgenes (encoding six sg-piRNAs) are required for silencing. Each pool can, therefore, target 60 genes and individual piRNAi transgenes can be ‘dialed out’ using 16 orthogonal primers (8 forward * 8 reverse = 64 unique combinations). Restriction sites (DraI or EcoRV) allow Sanger sequencing of pair-wise amplified piRNA transgenes. b. 96-well amplification of 60 different piRNA transgene pairs targeting him-5 using two rounds of PCR. The first PCR was a bulk amplification of all oligos in the pool using all 16 amplification primers concurrently. 60 specific piRNA transgenes were amplified in a second round of PCR performed with pair-wise orthogonal primers listed above wells (expected size = 300 bp, indicated by arrow). Control reactions contained no template to assess background amplification of contaminants. With no optimization, we were able to amplify 51 of 60 piRNA transgenes (nine wells with weak or no band at 300 bp). Ladder = 100–10,000 bp VersaLadder (GoldBio). For a large-scale library (for example, a whole-genome library) a subcloning step after the first bulk amplification and transformation of the amplified oligo pool into bacteria would maintain long-term integrity and facilitate distribution of the oligo-pool as lyophilized plasmid pools. c. Representative example of Sanger sequencing of a PCR-amplified piRNA transgene from the oligo pool. 12 of 12 sequenced PCR products contained the expected three unique sg-piRNAs. From the sequencing trace, a low level of cross-talk is visible (minor peaks below the three sg-piRNAs), which can likely be minimized by reducing the number of PCR cycles and using a sub-cloned library as a PCR template.

Journal: Nature methods

Article Title: Reprogramming the piRNA pathway for multiplexed and transgenerational gene silencing in C. elegans

doi: 10.1038/s41592-021-01369-z

Figure Lengend Snippet: a. Schematic of two different 300 bp piRNAi transgenes, each encoding three sg-piRNAs. 300 bp oligos synthesized at large scale on oligo chips are delivered as arrayed sub-pools with 121 unique oligos in each of 384 wells. The piRNA transgenes are flanked by orthogonal forward and reverse primer sites that allow ‘dial-out’ PCR of transgenes from complex oligo pools (Kosuri et al., 2010). Each oligo pool contains 121 different oligos with a maximum length of 300 base pairs (Matrix Oligo Pools, Twist Bioscience). Two piRNAi transgenes (encoding six sg-piRNAs) are required for silencing. Each pool can, therefore, target 60 genes and individual piRNAi transgenes can be ‘dialed out’ using 16 orthogonal primers (8 forward * 8 reverse = 64 unique combinations). Restriction sites (DraI or EcoRV) allow Sanger sequencing of pair-wise amplified piRNA transgenes. b. 96-well amplification of 60 different piRNA transgene pairs targeting him-5 using two rounds of PCR. The first PCR was a bulk amplification of all oligos in the pool using all 16 amplification primers concurrently. 60 specific piRNA transgenes were amplified in a second round of PCR performed with pair-wise orthogonal primers listed above wells (expected size = 300 bp, indicated by arrow). Control reactions contained no template to assess background amplification of contaminants. With no optimization, we were able to amplify 51 of 60 piRNA transgenes (nine wells with weak or no band at 300 bp). Ladder = 100–10,000 bp VersaLadder (GoldBio). For a large-scale library (for example, a whole-genome library) a subcloning step after the first bulk amplification and transformation of the amplified oligo pool into bacteria would maintain long-term integrity and facilitate distribution of the oligo-pool as lyophilized plasmid pools. c. Representative example of Sanger sequencing of a PCR-amplified piRNA transgene from the oligo pool. 12 of 12 sequenced PCR products contained the expected three unique sg-piRNAs. From the sequencing trace, a low level of cross-talk is visible (minor peaks below the three sg-piRNAs), which can likely be minimized by reducing the number of PCR cycles and using a sub-cloned library as a PCR template.

Article Snippet: DNA ladder: 100–10,000 bp VersaLadder (GoldBio).

Techniques: Amplification, Synthesized, Sequencing, Subcloning, Transformation Assay, Plasmid Preparation, Clone Assay