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All (3)
PromoCell
Santa Cruz
Genecopoeia
 

About 6 products / 40 snippets / 4 images

95 / 100
Bioz Stars
Preadipocyte Growth Medium
PromoCell  
Catalog#: C-27410
Techniques
Cell Culture, Recombinant, Incubation, Derivative Assay, Modification (see more)
Article Snippets
".. in PromoCell preadipocyte growth medium (PromoCell GmbH, Heidelberg, Germany) at 37 °C in a humidified 95% air and 5% CO2 .."  (see more)
93 / 100
Bioz vStars
sgRNA/Cas9 all-in-one expression clones targeting GLB1
Genecopoeia  
Catalog#: HCP327410-CG04-3-10
Description
"Transfect cells with our CRISPR plasmids with Cas9 and sgRNA for human, mouse, and rat. Search our database of more than 45,000 human, mouse, and rat genes for genome editing using CRISPR. sgRNA expression plasmids"  (see more)
95 / 100
Bioz Stars
Preadipocyte Growth Medium
PromoCell  
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Catalog#: C-27410
Techniques
Cell Culture, Recombinant, Incubation, Derivative Assay, Modification (see more)
Article Snippets
".. in PromoCell preadipocyte growth medium (PromoCell GmbH, Heidelberg, Germany) at 37 °C in a humidified 95% air and 5% CO2 .."  (see more)
95 / 100
Bioz Stars
Preadipocyte Growth Medium
PromoCell  
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Catalog#: C-39425
Techniques
 
Article Snippets
"emale abdominal subcutaneous adipose tissue of at least two different individuals were obtained from PromoCell (C-12730, Heidelberg, Germany). hPAd were cultivated in growth medium (PromoCell, C-27410)"  (see more)
Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white <t>preadipocytes</t> were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.
CELL-CTR
LC
MS
LC-MS
Knockdown of TBX15 in human primary preadipocytes significantly affects 130 genes (FDR < 0.05) in the WHRadjBMI co-expression network. A Illustration of TBX15 gene with introns and exons; and the relative RNA-seq read density in the human primary <t>preadipocyte</t> cells transfected with the negative control siRNA when compared to the cells transfected with the TBX15 siRNA. Scales for the read density are equal. B Bar plot showing the qPCR relative expression (2 −ddCt ) when compared to the housekeeping gene 36B4 and RNA-seq TPMs for TBX15 in the cells transfected with negative control siRNA when compared to the cells transfected with TBX15 siRNA ( n = 5). C Volcano plot of differentially expressed (DE) genes in TBX15 knockdown experiment, excluding TBX15 . Significant genes (FDR < 0.05) (dark gray), non-significant genes (light gray), and TFs (orange; FDR < 0.05) are plotted based on their log 10 p value and log 2 fold change in expression. Significantly differentially expressed TFs are labeled. Inlay shows the volcano plot of the TBX15 DE results with TBX15 included
EXP
RNAseq
TNSF
NEGA-C
LBLNG
Partial Least Squares Discriminant Analysis (PLS-DA) of proteins among the five stages. Stage-1: subcutaneous <t>preadipocytes,</t> stage-2: after inducing adipocyte differentiation, stages-3 to -5: from initiation of lipid droplet formation to appearance of mature subcutaneous adipocytes (dependent on lipid droplet amount and formation). The ellipses represent the 95% confidence interval. This figure was generated using MetaboAnalyst 5.0.
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92 / 100
Bioz Stars
Preadipocyte Growth Medium KIT
PromoCell  
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Catalog#: C-27417
Techniques
Cell Culture, Derivative Assay, In Vitro, Co-Culture Assay, Recombinant (see more)
Article Snippets
"Subsequently, media was replaced with differentiation media (S5) with 100 nM LDN193189, 100 nM Gamma Secretase inhibitor XX GSiXX (Millipore), 10 μM Alk5 inhibitor II, 1 μM T3 for 7 days."  (see more)
90 / 100
Bioz vStars
2 aminocarbonyl amino 3 phenylpropanoic acid
Santa Cruz  
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Catalog#: SC-274107
93 / 100
Bioz vStars
sgRNA/Cas9 all-in-one expression clones targeting GLB1
Genecopoeia  
star_border
Catalog#: HCP327410-CG04-3-10
Description
"Transfect cells with our CRISPR plasmids with Cas9 and sgRNA for human, mouse, and rat. Search our database of more than 45,000 human, mouse, and rat genes for genome editing using CRISPR. sgRNA expression plasmids"  (see more)
90 / 100
Bioz vStars
2 4 methylphenoxy methyl oxirane
Santa Cruz  
star_border
Catalog#: SC-274100