![High-throughput (HT)-compatible and nearly xeno-free synthesis of vascular networks and blood vessel organoids from fluorescently tagged human induced pluripotent stem cells (hiPSCs). A Schematic illustration of the new differentiation protocol and representative images for the main differentiation steps (scale bars: d-2, d0, d3, d5 = 100 µm; d8, d12 = 250 µm; d14, d17 = 500µm). B Shown are the steps of embedding the vascular aggregates in an Akura 96-well plate and transferring the vascular networks from the Akura 96-well plate to a PrimeSurface 96 Slit-well plate. C The use of PrimeSurface 96 Slit-well plates reduces the time required for medium exchange (left image). Akura 96-well plates allow aggregates to be embedded in small cavities, minimizing the matrix surrounding the vascular networks (black arrows) and allowing direct transfer of vascular networks (white arrows) to new plates without time-consuming manual extraction of the networks from the gel (middle and right images). D The new protocol is simple to handle and achieves high synthesis efficiency after minimal training. Shown are the efficiencies of three training runs. E The sprouting efficiency is maintained when fetal bovine serum (FBS) is replaced with human platelet lysate (hPL) or chemically defined Panexin CD (PCD). The total numbers of sufficiently sprouted networks and vascular aggregates with insufficient sprouting are written inside the bars. F,G HiPSC-derived vascular networks (F) and blood vessel organoids (G) differentiated with the HT-compatible and nearly xeno-free protocol consist of a complex network of <t>endothelial</t> cells (CD31) and associated pericytes (PDGFR-β) [representative images; scale bars: 50 µm (F); 200 µm (G)]. White arrowheads indicate angiogenic sprouts. G Perfusion of vascular networks with TMR-amino-dextran in OrganoPlate graft plates shows anastomoses between the GFP-labeled vascular networks and the HUVEC-derived vascular bed (top, white arrowheads) as well as correct formation and permeability of the vascular networks (bottom).](https://bio-rxiv-thumbnail-images-cdn.bioz.com/dois_ending_with_88/10__1101_slash_2024__12__04__626588/10__1101_slash_2024__12__04__626588___F1.large.thumbnail.jpg)
![Effect of endogenous NO on the increase in RE in the ETC at low [O2]. Increases in RE in the ETC are represented by the normalized sum of changes in the cytochrome redox states. Dotted lines indicate a 10% increase in RE and the corresponding [O2] (significantly different, P < 0.05, with and without l-NMMA). Data are the mean of six experiments. (A) Changes in RE against [O2] <t>for</t> <t>RAW</t> 246.7 cells in the absence (filled circles) or presence (open circles) of 750 μM l-NMMA. (B) Changes in RE against [O2] for <t>HUVEC</t> in the absence (filled triangles) or presence (open triangles) of 500 μM l-NMMA. Broken lines show the [O2] at which there is a 10% increase in RE.](https://pub-med-central-thumbnail-images-cdn.bioz.com/pub_med_central_ids_ending_with_9657/pmc00419657/pmc00419657__zpq0180447380003.thumbnail.jpg)
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PromoCell
Lifeline Cell Technology
About 2 products / 490 snippets / 46 images
 99 / 100 Bioz Stars | HUVEC-c Human Umbilical Vein Endothelial Cells PromoCell   Sponsored | Visit Supplier | ||||
Catalog#: C-12200 Techniques Cell Culture, Isolation, Incubation, Recombinant, Modification, Transfection (see more) Article Snippets "Human umbilical vein endothelial cells (HUVECs) were purchased from Promocell (Jiangyin, China) and cultured in endothelial cell growth medium (Promocell)." (see more) | × Open an account star_border Product associated with above image that matches your search query Article Snippet "" Figure Legend " " | |||||
99 / 100 Bioz Stars | HUVEC-c Human Umbilical Vein Endothelial Cells PromoCell | star_border | Visit Supplier | |||
Catalog#: C-12200 Techniques Cell Culture, Isolation, Incubation, Recombinant, Modification, Transfection (see more) Article Snippets "Human umbilical vein endothelial cells (HUVECs) were purchased from Promocell (Jiangyin, China) and cultured in endothelial cell growth medium (Promocell)." (see more) | × Open an account star_border Product associated with above image that matches your search query Article Snippet "" Figure Legend " " | |||||
86 / 100 Bioz Stars | C-12200, cat Lifeline Cell Technology | star_border | Visit Supplier | |||
Article Snippets "HUVECs were purchased from Lifeline cell technology (C-12200, cat. no. 10171-906)." (see more) | × | |||||
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