C-110 Search Results


92
Alomone Labs cyppa
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
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90
Applied Engineering Inc ionisation chamber c110 (0.6)
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
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90
Kikkoman Biochemifa lumitester c-110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
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Kikkoman Corporation lumi-tester c-110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
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Glen Research c-110: ho(ch2)6ss(ch2)6-5′-tcgtcg-3′-c3-5′-acgttcg-3′-c3-5′-agatgat-3′ c-110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
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90
Isogen Life Science kikkoman lumitester c-110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
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Sumitomo Dainippon electric injection machine se75ev-c110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
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New Brunswick Scientific electronic colony counter new brunswick scientific
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
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Cabot Corporation silica tgc110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
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AGC Inc polycarbonate substrate carboglass (registered trademark) c-110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
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Sairan Co cardiopulmonary monitoring device sairan model c110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
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JENOPTIK Inc climate chamber c110/200
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
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Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, CyPPA, or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three different Trpm 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test

Journal: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology

Article Title: Ethanol’s Effects on Transient Receptor Potential Channel Expression in Brain Microvascular Endothelial Cells

doi: 10.1007/s11481-018-9796-3

Figure Lengend Snippet: Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, CyPPA, or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three different Trpm 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test

Article Snippet: For Trpm 7 inhibition, three different Trpm 7 Channel Blockers, NS 8593, CyPPA and SKA-31 (Alomone Labs, Jerusalem, Israel) were added to the inserts at 50 μM, together with a tracer Sodium Fluorescein (NaF) at 10 μg/mL, and incubated in a humidified cell culture incubator at 37 °C with 5% CO 2 for 24 h. One hundred microliter samples from both the upper insert and the lower chamber were transferred to a clear 96-well plate.

Techniques: Permeability, Construct, In Vitro, Incubation