APR-021-F Search Results


fitc anti p2y 1 antibody  (Alomone Labs)
93
Alomone Labs fitc anti p2y 1 antibody
Tissue taken from pups of PRYC x PRYP parents was used in PCR to determine offspring homozygous for <t>P2Y</t> 1 - LoxP flanked allele and hemizygous for PF4- cre (test mice) and offspring homozygous for P2Y 1 - LoxP flanked allele and a non carrier for PF4- cre (control ‘wild type’ mice). Representative PCR for LoxP and cre is shown of a litter ( A ). Platelets were taken via cardiac puncture and stained for with anti-CD41-PE conjugated antibody (platelet marker) and anti-P2Y 1 -FITC conjugated antibody to elucidate P2Y 1 expression between ‘test’ (Plt-P2Y 1 -/- ) and control (WT) platelets ( B ). In other experiments, platelets were harvested from blood, and leukocytes harvested from bone marrow of donor mice and their ability to migrate to fMLP (30nM) was measured using a transwell system ( C ) platelets, co-incubated with 100nM ADP, ( D ) neutrophils. Data expressed as means +/-SEM. n=3 ( B ) or 5-6 ( C , D ) per group. Significant difference represented: * P <0.05, ** P <0.01.
Fitc Anti P2y 1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc anti p2y 1 antibody - by Bioz Stars, 2026-04
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Tissue taken from pups of PRYC x PRYP parents was used in PCR to determine offspring homozygous for P2Y 1 - LoxP flanked allele and hemizygous for PF4- cre (test mice) and offspring homozygous for P2Y 1 - LoxP flanked allele and a non carrier for PF4- cre (control ‘wild type’ mice). Representative PCR for LoxP and cre is shown of a litter ( A ). Platelets were taken via cardiac puncture and stained for with anti-CD41-PE conjugated antibody (platelet marker) and anti-P2Y 1 -FITC conjugated antibody to elucidate P2Y 1 expression between ‘test’ (Plt-P2Y 1 -/- ) and control (WT) platelets ( B ). In other experiments, platelets were harvested from blood, and leukocytes harvested from bone marrow of donor mice and their ability to migrate to fMLP (30nM) was measured using a transwell system ( C ) platelets, co-incubated with 100nM ADP, ( D ) neutrophils. Data expressed as means +/-SEM. n=3 ( B ) or 5-6 ( C , D ) per group. Significant difference represented: * P <0.05, ** P <0.01.

Journal: bioRxiv

Article Title: Platelet-specific P2Y 1 receptor deficient mice have suppressed leukocyte recruitment in response to lipopolysaccharide

doi: 10.1101/2024.11.04.621858

Figure Lengend Snippet: Tissue taken from pups of PRYC x PRYP parents was used in PCR to determine offspring homozygous for P2Y 1 - LoxP flanked allele and hemizygous for PF4- cre (test mice) and offspring homozygous for P2Y 1 - LoxP flanked allele and a non carrier for PF4- cre (control ‘wild type’ mice). Representative PCR for LoxP and cre is shown of a litter ( A ). Platelets were taken via cardiac puncture and stained for with anti-CD41-PE conjugated antibody (platelet marker) and anti-P2Y 1 -FITC conjugated antibody to elucidate P2Y 1 expression between ‘test’ (Plt-P2Y 1 -/- ) and control (WT) platelets ( B ). In other experiments, platelets were harvested from blood, and leukocytes harvested from bone marrow of donor mice and their ability to migrate to fMLP (30nM) was measured using a transwell system ( C ) platelets, co-incubated with 100nM ADP, ( D ) neutrophils. Data expressed as means +/-SEM. n=3 ( B ) or 5-6 ( C , D ) per group. Significant difference represented: * P <0.05, ** P <0.01.

Article Snippet: FITC-anti-P2Y 1 antibody (Cat #APR-021-F) was purchased from Alomone Labs. Stromatol (Cat. #321200S) was purchased from Mascia Brunelli, Italy.

Techniques: Control, Staining, Marker, Expressing, Incubation