ANP-002 Search Results


antibody against α2 nka  (Alomone Labs)
94
Alomone Labs antibody against α2 nka
List of the primers.
Antibody Against α2 Nka, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody against α2 nka - by Bioz Stars, 2026-04
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List of the primers.

Journal: Biomedicines

Article Title: HIF-2α Controls Expression and Intracellular Trafficking of the α2-Subunit of Na,K-ATPase in Hypoxic H9c2 Cardiomyocytes

doi: 10.3390/biomedicines11112879

Figure Lengend Snippet: List of the primers.

Article Snippet: The antibody against α2-NKA (rabbit, #ANP-002, 1:1000 dilution) was from Alomone Labs and beta-actin, used to normalize the band densities as a housekeeping gene (mouse, #A2228, 1:10000 dilution), was from Sigma.

Techniques:

Effects of hypoxia and HIF silencing on α2-NKA mRNA expression. H9c2 cells were infected with 100 MOI shHIF-1α and shHIF-2α containing adenoviral vectors and kept in normoxia (19% O 2 ) or hypoxia (1% O 2 ) for 24 h. mRNA expression was normalized to 28SrRNA, and the results are given as the normalized values of scr.co. Mean values ± SD of four independent experiments as shown. The level of significance was set to p < 0.05: * effect of hypoxia compared to normoxia, + effect of HIF-2α silencing in hypoxic cells compared to hypoxic scr.co. scr.co: scrambled control; shHIF-1α: HIF-1α-silenced cells; shHIF-2α: HIF-2α-silenced cells.

Journal: Biomedicines

Article Title: HIF-2α Controls Expression and Intracellular Trafficking of the α2-Subunit of Na,K-ATPase in Hypoxic H9c2 Cardiomyocytes

doi: 10.3390/biomedicines11112879

Figure Lengend Snippet: Effects of hypoxia and HIF silencing on α2-NKA mRNA expression. H9c2 cells were infected with 100 MOI shHIF-1α and shHIF-2α containing adenoviral vectors and kept in normoxia (19% O 2 ) or hypoxia (1% O 2 ) for 24 h. mRNA expression was normalized to 28SrRNA, and the results are given as the normalized values of scr.co. Mean values ± SD of four independent experiments as shown. The level of significance was set to p < 0.05: * effect of hypoxia compared to normoxia, + effect of HIF-2α silencing in hypoxic cells compared to hypoxic scr.co. scr.co: scrambled control; shHIF-1α: HIF-1α-silenced cells; shHIF-2α: HIF-2α-silenced cells.

Article Snippet: The antibody against α2-NKA (rabbit, #ANP-002, 1:1000 dilution) was from Alomone Labs and beta-actin, used to normalize the band densities as a housekeeping gene (mouse, #A2228, 1:10000 dilution), was from Sigma.

Techniques: Expressing, Infection, Control

Effect of hypoxia and HIF silencing on the abundance of α2-NKA in the membranes and cytoplasmic fractions of H9c2 cells. Plasma membrane and intracellular α2-NKA protein expression of H9c2 cells silenced with shHIFs and kept in normoxia or hypoxia. The biotinylated fraction is considered the membrane fraction, and the non-biotinylated is considered the intracellular fraction. A representative blot showing α2-NKA ( a ) surface expression (biotinylated) and ( b ) intracellular expression (non-biotinylated). Beta-actin was not detected in the surface (biotinylated) membrane fraction but only in the intracellular pool. Mean values ± SD of four independent experiments as shown. The level of significance was set at p < 0.05: * effect of hypoxia compared to normoxia, + effect of HIF-2α silencing in hypoxic cells compared to hypoxic scr.co. scr.co: scrambled control; shHIF-1α: HIF-1α-silenced cells; shHIF-2α: HIF-2α-silenced cells.

Journal: Biomedicines

Article Title: HIF-2α Controls Expression and Intracellular Trafficking of the α2-Subunit of Na,K-ATPase in Hypoxic H9c2 Cardiomyocytes

doi: 10.3390/biomedicines11112879

Figure Lengend Snippet: Effect of hypoxia and HIF silencing on the abundance of α2-NKA in the membranes and cytoplasmic fractions of H9c2 cells. Plasma membrane and intracellular α2-NKA protein expression of H9c2 cells silenced with shHIFs and kept in normoxia or hypoxia. The biotinylated fraction is considered the membrane fraction, and the non-biotinylated is considered the intracellular fraction. A representative blot showing α2-NKA ( a ) surface expression (biotinylated) and ( b ) intracellular expression (non-biotinylated). Beta-actin was not detected in the surface (biotinylated) membrane fraction but only in the intracellular pool. Mean values ± SD of four independent experiments as shown. The level of significance was set at p < 0.05: * effect of hypoxia compared to normoxia, + effect of HIF-2α silencing in hypoxic cells compared to hypoxic scr.co. scr.co: scrambled control; shHIF-1α: HIF-1α-silenced cells; shHIF-2α: HIF-2α-silenced cells.

Article Snippet: The antibody against α2-NKA (rabbit, #ANP-002, 1:1000 dilution) was from Alomone Labs and beta-actin, used to normalize the band densities as a housekeeping gene (mouse, #A2228, 1:10000 dilution), was from Sigma.

Techniques: Membrane, Expressing, Control