Journal: Asian Journal of Andrology

Article Title: Circadian disturbance induces erectile dysfunction by impairing endothelial function

doi: 10.4103/aja202345

Figure Lengend Snippet: Primer sequences of real-time quantitative polymerase chain reaction

Article Snippet: After blocking with 5% powdered skim milk in Tris-buffered saline with Tween (TBS-T) for 1 h at room temperature, the membranes were incubated with primary antibodies against endothelial nitric oxide synthase (eNOS; 1:800, ab76198; Abcam, Cambridge, UK), neuronal nitric oxide synthase (nNOS; 1:800, ab3511; Abcam), CLOCK (1:1000, A7265; ABclonal Technology, Wuhan, China), BMAL1 (1:1000, A4714; ABclonal Technology), PER1/2/3 (1:1000/1000/1000, A8449/A3217/A2219; ABclonal Technology), CRY1/2 (1:1000/400, A13662/A17465; ABclonal Technology), and REV-ERBα (1:1000, A20452; ABclonal Technology) for 24 h at 4°C.

Techniques: Sequencing

Journal: Asian Journal of Andrology

Article Title: Circadian disturbance induces erectile dysfunction by impairing endothelial function

doi: 10.4103/aja202345

Figure Lengend Snippet: Circadian elements of corpora cavernosa are changed by light/dark alteration. ( a ) Representative western blot images for PER1, and statistical analysis for PER1 and Per1 . ( b ) Representative western blot images for PER2, and statistical analysis for PER2 and Per2 . ( c ) Representative western blot images for PER3, and statistical analysis for PER3 and Per3 . ( d ) Representative western blot images for CRY1, and statistical analysis for CRY1 and Cry1 . ( e ) Representative western blot images for CRY2, and statistical analysis for CRY2 and Cry2 . * P < 0.05, ** P < 0.01, *** P < 0.001. The definition of different groups is shown in . PER: period; CRY: cryptochrome; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: After blocking with 5% powdered skim milk in Tris-buffered saline with Tween (TBS-T) for 1 h at room temperature, the membranes were incubated with primary antibodies against endothelial nitric oxide synthase (eNOS; 1:800, ab76198; Abcam, Cambridge, UK), neuronal nitric oxide synthase (nNOS; 1:800, ab3511; Abcam), CLOCK (1:1000, A7265; ABclonal Technology, Wuhan, China), BMAL1 (1:1000, A4714; ABclonal Technology), PER1/2/3 (1:1000/1000/1000, A8449/A3217/A2219; ABclonal Technology), CRY1/2 (1:1000/400, A13662/A17465; ABclonal Technology), and REV-ERBα (1:1000, A20452; ABclonal Technology) for 24 h at 4°C.

Techniques: Western Blot