A19473 Search Results


ddab  (Chem Impex International)
95
Chem Impex International ddab
Ddab, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-tom20 a19403  (ABclonal Biotechnology)
90
ABclonal Biotechnology anti-tom20 a19403
Anti Tom20 A19403, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti tom20  (Proteintech)
96
Proteintech anti tom20
Anti Tom20, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tomm20  (ABclonal Biotechnology)
90
ABclonal Biotechnology tomm20
Tomm20, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a19403 anti tollip proteintech  (Proteintech)
93
Proteintech a19403 anti tollip proteintech
A19403 Anti Tollip Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit antitomm20  (ABclonal Biotechnology)
90
ABclonal Biotechnology rabbit antitomm20
Rabbit Antitomm20, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tom20  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc tom20
(A) Determination of the submitochondrial localization of FBXL4 by Protease K and trypsin digestion. Mitochondria from HeLa FBXL4-FLAG stable line were purified and stored as intact mitochondria or treated with hypotonic swelling buffer or lysed with Triton X-100 buffer. Different mitochondrial preparations were then digested with Protease K or trypsin. (B) Analysis of the association of FBXL4 with membrane by alkaline carbonate extraction. Purified mitochondria from HeLa FBXL4-FLAG stable line were treated with alkaline carbonate buffer at the indicated pH and then centrifuged to collect the supernatant and the pellet fractions. S: supernatant; P: pellet. (C) “DAS” prediction of the transmembrane domain (TMD) of FBXL4. The red box highlights the putative TMD at FBXL4 N-terminus. (D) Analysis of the TMD and the flanking sequences of FBXL4, <t>Tom20</t> and Tom70. Positively-charged residues are highlighted in red; negatively-charged residues are highlighted in green. H.s. : Homosapien; R.n. : Rattus norvegicus. (E) Identification of the mitochondrial targeting sequence of FBXL4. Indicated FBXL4 N-terminal sequences were fused to RFP and expressed in HeLa cells to analyze their subcellular localization. (F) Determination of the submitochondrial localization of FBXL4(1-23)-RFP by Protease K digestion. Experiments were performed similarly as (A).
Tom20, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tom20 - by Bioz Stars, 2026-04
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anti smad5 (a1947)  (ABclonal Biotechnology)
90
ABclonal Biotechnology anti smad5 (a1947)
(A) Determination of the submitochondrial localization of FBXL4 by Protease K and trypsin digestion. Mitochondria from HeLa FBXL4-FLAG stable line were purified and stored as intact mitochondria or treated with hypotonic swelling buffer or lysed with Triton X-100 buffer. Different mitochondrial preparations were then digested with Protease K or trypsin. (B) Analysis of the association of FBXL4 with membrane by alkaline carbonate extraction. Purified mitochondria from HeLa FBXL4-FLAG stable line were treated with alkaline carbonate buffer at the indicated pH and then centrifuged to collect the supernatant and the pellet fractions. S: supernatant; P: pellet. (C) “DAS” prediction of the transmembrane domain (TMD) of FBXL4. The red box highlights the putative TMD at FBXL4 N-terminus. (D) Analysis of the TMD and the flanking sequences of FBXL4, <t>Tom20</t> and Tom70. Positively-charged residues are highlighted in red; negatively-charged residues are highlighted in green. H.s. : Homosapien; R.n. : Rattus norvegicus. (E) Identification of the mitochondrial targeting sequence of FBXL4. Indicated FBXL4 N-terminal sequences were fused to RFP and expressed in HeLa cells to analyze their subcellular localization. (F) Determination of the submitochondrial localization of FBXL4(1-23)-RFP by Protease K digestion. Experiments were performed similarly as (A).
Anti Smad5 (A1947), supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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albendazole cat. no. a1943  (Tokyo Chemical Industry)
90
Tokyo Chemical Industry albendazole cat. no. a1943
(A) Determination of the submitochondrial localization of FBXL4 by Protease K and trypsin digestion. Mitochondria from HeLa FBXL4-FLAG stable line were purified and stored as intact mitochondria or treated with hypotonic swelling buffer or lysed with Triton X-100 buffer. Different mitochondrial preparations were then digested with Protease K or trypsin. (B) Analysis of the association of FBXL4 with membrane by alkaline carbonate extraction. Purified mitochondria from HeLa FBXL4-FLAG stable line were treated with alkaline carbonate buffer at the indicated pH and then centrifuged to collect the supernatant and the pellet fractions. S: supernatant; P: pellet. (C) “DAS” prediction of the transmembrane domain (TMD) of FBXL4. The red box highlights the putative TMD at FBXL4 N-terminus. (D) Analysis of the TMD and the flanking sequences of FBXL4, <t>Tom20</t> and Tom70. Positively-charged residues are highlighted in red; negatively-charged residues are highlighted in green. H.s. : Homosapien; R.n. : Rattus norvegicus. (E) Identification of the mitochondrial targeting sequence of FBXL4. Indicated FBXL4 N-terminal sequences were fused to RFP and expressed in HeLa cells to analyze their subcellular localization. (F) Determination of the submitochondrial localization of FBXL4(1-23)-RFP by Protease K digestion. Experiments were performed similarly as (A).
Albendazole Cat. No. A1943, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mek162 a1947  (ApexBio)
90
ApexBio mek162 a1947

Mek162 A1947, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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smad5  (Proteintech)
93
Proteintech smad5

Smad5, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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smad5 - by Bioz Stars, 2026-04
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3-(2-hydroxyethyl)-1-(6-{[(2-hydroxyethyl)carbamoyl]amino}hexyl)urea  (Biosynth Carbosynth)
N/A
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Image Search Results


(A) Determination of the submitochondrial localization of FBXL4 by Protease K and trypsin digestion. Mitochondria from HeLa FBXL4-FLAG stable line were purified and stored as intact mitochondria or treated with hypotonic swelling buffer or lysed with Triton X-100 buffer. Different mitochondrial preparations were then digested with Protease K or trypsin. (B) Analysis of the association of FBXL4 with membrane by alkaline carbonate extraction. Purified mitochondria from HeLa FBXL4-FLAG stable line were treated with alkaline carbonate buffer at the indicated pH and then centrifuged to collect the supernatant and the pellet fractions. S: supernatant; P: pellet. (C) “DAS” prediction of the transmembrane domain (TMD) of FBXL4. The red box highlights the putative TMD at FBXL4 N-terminus. (D) Analysis of the TMD and the flanking sequences of FBXL4, Tom20 and Tom70. Positively-charged residues are highlighted in red; negatively-charged residues are highlighted in green. H.s. : Homosapien; R.n. : Rattus norvegicus. (E) Identification of the mitochondrial targeting sequence of FBXL4. Indicated FBXL4 N-terminal sequences were fused to RFP and expressed in HeLa cells to analyze their subcellular localization. (F) Determination of the submitochondrial localization of FBXL4(1-23)-RFP by Protease K digestion. Experiments were performed similarly as (A).

Journal: bioRxiv

Article Title: A mitochondrial SCF-FBXL4 ubiquitin E3 ligase complex restrains excessive mitophagy to prevent mitochondrial disease

doi: 10.1101/2022.11.11.516094

Figure Lengend Snippet: (A) Determination of the submitochondrial localization of FBXL4 by Protease K and trypsin digestion. Mitochondria from HeLa FBXL4-FLAG stable line were purified and stored as intact mitochondria or treated with hypotonic swelling buffer or lysed with Triton X-100 buffer. Different mitochondrial preparations were then digested with Protease K or trypsin. (B) Analysis of the association of FBXL4 with membrane by alkaline carbonate extraction. Purified mitochondria from HeLa FBXL4-FLAG stable line were treated with alkaline carbonate buffer at the indicated pH and then centrifuged to collect the supernatant and the pellet fractions. S: supernatant; P: pellet. (C) “DAS” prediction of the transmembrane domain (TMD) of FBXL4. The red box highlights the putative TMD at FBXL4 N-terminus. (D) Analysis of the TMD and the flanking sequences of FBXL4, Tom20 and Tom70. Positively-charged residues are highlighted in red; negatively-charged residues are highlighted in green. H.s. : Homosapien; R.n. : Rattus norvegicus. (E) Identification of the mitochondrial targeting sequence of FBXL4. Indicated FBXL4 N-terminal sequences were fused to RFP and expressed in HeLa cells to analyze their subcellular localization. (F) Determination of the submitochondrial localization of FBXL4(1-23)-RFP by Protease K digestion. Experiments were performed similarly as (A).

Article Snippet: BNIP3 (Abcam, ab109362; Abcam, ab10433; Cell signaling, 3769S), NIX (Cell signaling, 12396S), FUNDC1 (From Quan Chen lab), FLAG (Sigma-Aldrich, F1804), HA (Sigma-Aldrich, H6533), ACTIN (Sigma-Aldrich, A2066), TOM70 (Proteintech, 14528-1-AP), TOM20 (ABclonal, A19403), SMAC (Cell signaling, 2954S), MITOFILIN (Proteintech, 10179-1-AP), HSP60 (Cell signaling, 4870S), TIMM23(Proteintech, 11123-1-AP), SKP1 (Cell signaling, 2156S), CUL1 (Proteintech, 12895-1-AP), Total OXPHOS Cocktail (Abcam, ab110411), Cytochrome C (BD Biosciences, 556433), mCherry (Easybio, BE2026), MTCH2 (Proteintech, 16888-1-AP), BECLIN1 (Cell signaling, 3495T), FIP200 (ABclonal, A14685), TFAM (Abcam, ab131607), LONP1 (Proteintech, 15440-1-AP), VDAC (Cell signaling, 4866S), Donkey polyclonal anti-Rabbit IgG (H+L),HRP-conjugated (Jackson ImmunoResearch, 711-035-152), Donkey polyclonal anti-Mouse IgG (H+L), HRP-conjugated (Jackson ImmunoResearch, 711-035-151), Goat polyclonal anti-Mouse IgG, Fcγ fragment specific, HRP-conjugated (Jackson ImmunoResearch, 115-035-008), Goat polyclonal anti-Mouse IgG, light chain specific, HRP-conjugated (Jackson ImmunoResearch, 115-035-174).

Techniques: Purification, Membrane, Extraction, Sequencing

Journal: The EMBO Journal

Article Title: Reciprocal priming between receptor tyrosine kinases at recycling endosomes orchestrates cellular signalling outputs

doi: 10.15252/embj.2020107182

Figure Lengend Snippet:

Article Snippet: MEK162 , APEXBIO , A1947.

Techniques: Virus, Recombinant, Extraction, Multiplex sample analysis, Transfection, Molecular Weight, Marker, Reverse Transcription, Protease Inhibitor, Membrane, Cell Culture, Clinical Proteomics, Spectroscopy, Imaging, cDNA Synthesis, Plasmid Preparation, Negative Control, Mutagenesis, Clone Assay, Generated, Software, Microscopy