Journal: PLoS ONE

Article Title: Central Role for MCP-1/CCL2 in Injury-Induced Inflammation Revealed by In Vitro , In Silico , and Clinical Studies

doi: 10.1371/journal.pone.0079804

Figure Lengend Snippet: Primary hepatocytes isolated from wild-type and MCP-1 −/− mice (n = 3 each) were cultured under normoxic and hypoxic conditions for 48 h in three independent experiments. The cells were then fixed and processed for confocal immunofluorescence imaging as described in the Materials and Methods . ( A ) Fluorescent labeling: Phalloidin (white), MCP-1 (red), IL-6 (green), Hoechst (blue). ( B ) Quantification of immunostained cells (fluorescence intensity from 600–700 hepatocytes from 3 independent fields, n = 3 coverslips/experiment). Results are the mean ± SEM (* P <0.001 wild-type vs. MCP-1 −/− , analyzed by t -test).

Article Snippet: For immunostaining the following primary and secondary antibodies (diluted in PBS containing 1% donkey serum) were used: LEAF™ Purified anti-mouse MCP-1 antibody (1∶2000; Cat. No. 505905, BioLegend, San Diego, CA), rabbit polyclonal anti-IL-6 antibody (1∶2000; Cat. No. ab6672, Abcam), Cy3-conjugated anti-mouse (1∶1000; Cat. No. 715-165-151, Jackson-ImmunoResearch Laboratories, West Grove, PA), Alexa Fluor-conjugated 647 anti-rabbit (1∶1000; Cat. No. A31573, Invitrogen, Grand Island, NY), and 488-conjugated anti-phalloidin (1∶1000; Cat. No. A12379, Invitrogen).

Techniques: Isolation, Cell Culture, Immunofluorescence, Imaging, Labeling, Fluorescence

Journal: Disease Markers

Article Title: Qing Zao Fang (QZF) Alleviates the Inflammatory Microenvironment of the Submandibular Gland in Sjögren's Syndrome Based on the PI3K/Akt/HIF-1 α /VEGF Signaling Pathway

doi: 10.1155/2022/6153459

Figure Lengend Snippet: The primers for RT-qPCR.

Article Snippet: The catalogue number and brand of used primary antibodies were listed in the following: anti-PI3K (4228T, ABclonal), anti-p-PI3K (4228T, CST), anti-AKT (A17909, ABclonal), anti-p-AKT (4060T, ABclonal), anti-HIF-1 α (A16873, ABclonal), anti-VEGF (A12303, ABclonal), anti-IL-17A (A0688, ABclonal), anti-LC3 I/II (A19665, ABclonal), and β -actin (1 : 2000, AC026, ABclonal).

Techniques:

Journal: Disease Markers

Article Title: Qing Zao Fang (QZF) Alleviates the Inflammatory Microenvironment of the Submandibular Gland in Sjögren's Syndrome Based on the PI3K/Akt/HIF-1 α /VEGF Signaling Pathway

doi: 10.1155/2022/6153459

Figure Lengend Snippet: (a) The strip chart of protein expression in six groups detected by western blot. The expression of β -actin was serving as the internal reference to relatively quantify the protein expression by densitometry. (b–e) The column diagram of protein expression detected by western blot. (b) PI3K and phosphorylated PI3K; (c) AKT and phosphorylated AKT; (d) HIF-1 α and (e) VEGF. Error bars represent SD, n = 3. Calculation of statistical significance was performed in each group compared with SS, ∗∗ P < 0.01 and ∗∗∗ P < 0.001. ## P < 0.01 and ### P < 0.001 QZF-treated group versus hydroxychloroquine.

Article Snippet: The catalogue number and brand of used primary antibodies were listed in the following: anti-PI3K (4228T, ABclonal), anti-p-PI3K (4228T, CST), anti-AKT (A17909, ABclonal), anti-p-AKT (4060T, ABclonal), anti-HIF-1 α (A16873, ABclonal), anti-VEGF (A12303, ABclonal), anti-IL-17A (A0688, ABclonal), anti-LC3 I/II (A19665, ABclonal), and β -actin (1 : 2000, AC026, ABclonal).

Techniques: Stripping Membranes, Expressing, Western Blot