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Image Search Results
Journal: Scandinavian journal of immunology
Article Title: The expression and significance of TIPE2 in peripheral blood mononuclear cells from asthmatic children.
doi: 10.1111/sji.12110
Figure Lengend Snippet: Figure 1 The expression levels of TIPE2 mRNA in children with asthma and healthy controls. (A) TIPE2 mRNA expression in peripheral blood mononuclear cells from 42 children with asthma and 39 healthy controls was determined by semi-quantitative RT-PCR. The results showed that the expression of TIPE2 mRNA was reduced in patients with asthma compared with normal controls. (B) The expression of TIPE2 mRNA in 42 patients and 39 controls was determined by quantitative real-time PCR as described previously. The results showed that the expression level of TIPE2 mRNA in the children with asthma was significantly lower than that in healthy subjects (P = 0.0323).
Article Snippet: After blocking with 2% BSA in TBST containing 0.1% Tween-20 for 1 h at room temperature, the membrane was incubated overnight at 4 °C with 1:300 dilution of
Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction
Journal: Scandinavian journal of immunology
Article Title: The expression and significance of TIPE2 in peripheral blood mononuclear cells from asthmatic children.
doi: 10.1111/sji.12110
Figure Lengend Snippet: Figure 2 The expression of TIPE2 protein in peripheral blood mononuclear cells from 42 children with asthma and 39 healthy controls was detected by Western blot. b-actin was used as a loading control to assure equal amounts of protein in all lanes. The results of Western blot revealed that the expression of TIPE2 protein was lower in patients with asthma compared with normal controls.
Article Snippet: After blocking with 2% BSA in TBST containing 0.1% Tween-20 for 1 h at room temperature, the membrane was incubated overnight at 4 °C with 1:300 dilution of
Techniques: Expressing, Western Blot, Control
Journal: Scandinavian journal of immunology
Article Title: The expression and significance of TIPE2 in peripheral blood mononuclear cells from asthmatic children.
doi: 10.1111/sji.12110
Figure Lengend Snippet: Figure 5 Correlations of TIPE2 mRNA expression with IL-4, IFN-c, IgE and eosinophil in children with asthma. (A) There was a significantly negative correlation between TIPE2 mRNA expression and serum IL-4 level in all the patients with asthma (r = 0.3693, P = 0.0344). (B) There was a significantly negative correlation between TIPE2 mRNA expression and serum total IgE level in all the patients with asthma (r = 0.5173, P = 0.001). (C) There was a significantly negative correlation between TIPE2 mRNA expression and eosinophil count in all the patients with asthma (r = 0.3503, P = 0.0362). (D) There was no statistically significant correlation between TIPE2 mRNA expression and serum IFN-c level in all the patients with asthma (r = 0.1504, P = 0.3959).
Article Snippet: After blocking with 2% BSA in TBST containing 0.1% Tween-20 for 1 h at room temperature, the membrane was incubated overnight at 4 °C with 1:300 dilution of
Techniques: Expressing