Journal: Oxidative Medicine and Cellular Longevity

Article Title: Polysaccharides from Garlic Protect against Liver Injury in DSS-Induced Inflammatory Bowel Disease of Mice via Suppressing Pyroptosis and Oxidative Damage

doi: 10.1155/2022/2042163

Figure Lengend Snippet: PSG inhibited oxidative stress of the liver tissues in IBD mice. Effect of PSG on the levels of ROS (a) and MDA (b) in the liver tissues of IBD mice. The protein expressions of the Nrf2/Keap1/HO1/NQO1 pathway in the liver tissues were determined by Western blot assay (c) and quantitative analysis for Western blot results ( n = 3) (d). (e) The protein expression of Nrf2 in the liver tissues was detected by IHC. Results were expressed as the mean ± SEM. ∗ p < 0.05 and ∗∗ p < 0.01 vs. the control group; # p < 0.05 and ## p < 0.01 vs. the model group (DSS only).

Article Snippet: Primary antibodies against Nrf2 (A0674), NLRP3 (A5652), ASC (A1170), and phospho-histone H2AX-S139 (AP0099) were obtained from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and TLR4 (293072) was from Santa Cruz Biotechnology Inc. (California, USA), respectively.

Techniques: Western Blot, Expressing, Control

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Polysaccharides from Garlic Protect against Liver Injury in DSS-Induced Inflammatory Bowel Disease of Mice via Suppressing Pyroptosis and Oxidative Damage

doi: 10.1155/2022/2042163

Figure Lengend Snippet: Proposed mechanism of PSG in treating DSS-induced IBD and SLI of mice. Polysaccharides of garlic (PSG); interleukin-1 β (IL-1 β ); interleukin 18 (IL-18); reactive oxygen species (ROS), malondialdehyde (MDA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), nuclear factor-k-gene binding (NF- κ B), nuclear factor E2-related factor 2 (Nrf2), Kelch-like ECH associated protein 1 (Keap1), heme oxygenase 1 (HO1), myeloperoxidase (MPO), diamine oxidase (DAO), lipopolysaccharide (LPS), upregulation (↑), and downregulation (↓).

Article Snippet: Primary antibodies against Nrf2 (A0674), NLRP3 (A5652), ASC (A1170), and phospho-histone H2AX-S139 (AP0099) were obtained from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and TLR4 (293072) was from Santa Cruz Biotechnology Inc. (California, USA), respectively.

Techniques: Binding Assay

Journal: PLOS ONE

Article Title: Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway

doi: 10.1371/journal.pone.0296792

Figure Lengend Snippet: (A-E) Representative blot images and quantitative analysis of LKB1, AMPK, Nrf2, HO-1. (F-G) Representative blot images and quantitative analysis of Nuclear-Nrf2. (H-I) Representative images of Nuclear-Nrf2 and the quantitative analysis for Nuclear-Nrf2 (scale bar, 100 μm). n = 3. Data are presented as means ± S.E.M. * p < 0.05 and ** p < 0.01 vs. db/m group; Φ p < 0.05 and ΦΦ p < 0.01 vs. db/db group.

Article Snippet: The frozen sections of the myocardium of mice were a sealed with 5% BSA blocking buffer for 1h at room temperature, GLUT4 (1:100, 66846-1-Ig, Proteintech, China) and Nrf2 (1:100, A0674, BOSTER, China) antibody was diluted and dropped onto the sections, and incubated at room temperature for 2 h. PBS was washed, and the diluted fluorescently labeled secondary antibody was added for 1h at 37 ̊C for protection from light, and the nuclear was stained with diluted DAPI.

Techniques:

Journal: PLOS ONE

Article Title: Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway

doi: 10.1371/journal.pone.0296792

Figure Lengend Snippet: (A) The cell viability of H9C2 cells was treated with HG with 0, 10, 30, and 50mM for 24 and 48 h. (B) The H9C2 cells were treated with IH and HG for 24 and 48 h, and cell viability of H9C2 cells was measured. (C) The cell viability of H9C2 cells treated with IH and HG. n = 6. (D-E) Representative images of mitochondrial membrane potential and quantitative analysis of the JC-1 ratio. n = 4. (F-G) Representative images of ROS and quantitative analysis of ROS. (H-K) Representative blot images and quantitative analysis of LKB1, AMPK, and Nrf2. n = 3. Data are presented as means ± S.E.M. * p < 0.05 and ** p < 0.01 vs. CON group; Φ p < 0.05 and ΦΦ p < 0.01 vs. HG group.

Article Snippet: The frozen sections of the myocardium of mice were a sealed with 5% BSA blocking buffer for 1h at room temperature, GLUT4 (1:100, 66846-1-Ig, Proteintech, China) and Nrf2 (1:100, A0674, BOSTER, China) antibody was diluted and dropped onto the sections, and incubated at room temperature for 2 h. PBS was washed, and the diluted fluorescently labeled secondary antibody was added for 1h at 37 ̊C for protection from light, and the nuclear was stained with diluted DAPI.

Techniques: Membrane

Journal: PLOS ONE

Article Title: Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway

doi: 10.1371/journal.pone.0296792

Figure Lengend Snippet: (A-B) Representative images of mitochondrial membrane potential and quantitative analysis of the JC-1 ratio. (C-D) Representative images and quantitative analysis of ROS. (E-G) Representative blot images and quantitative analysis of AMPK and Nrf2. n = 3. Data are presented as means ± S.E.M. * p < 0.05 and ** p < 0.01 vs. CON group; Φ p < 0.05 and ΦΦ p < 0.01 vs. IH+HG group.

Article Snippet: The frozen sections of the myocardium of mice were a sealed with 5% BSA blocking buffer for 1h at room temperature, GLUT4 (1:100, 66846-1-Ig, Proteintech, China) and Nrf2 (1:100, A0674, BOSTER, China) antibody was diluted and dropped onto the sections, and incubated at room temperature for 2 h. PBS was washed, and the diluted fluorescently labeled secondary antibody was added for 1h at 37 ̊C for protection from light, and the nuclear was stained with diluted DAPI.

Techniques: Membrane

Journal: PLOS ONE

Article Title: Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway

doi: 10.1371/journal.pone.0296792

Figure Lengend Snippet: (A-E) Representative blot images and quantitative analysis of LKB1, AMPK, Nrf2, HO-1. (F-G) Representative blot images and quantitative analysis of Nuclear-Nrf2. (H-I) Representative images of Nuclear-Nrf2 and the quantitative analysis for Nuclear-Nrf2 (scale bar, 100 μm). n = 3. Data are presented as means ± S.E.M. * p < 0.05 and ** p < 0.01 vs. db/m group; Φ p < 0.05 and ΦΦ p < 0.01 vs. db/db group.

Article Snippet: Seal with 5% skim milk powder sealing solution for 2 h, adding primary antibody and incubating overnight at 4°C: Tubulin (1:10000, GTX628802) and Caspase-3(1:1000, #14220) from CST; p-AMPK (1:2000, AP0116) and HO-1(1:1000, A1346) from ABclonal; PI3K (1:1000, AF6241) from Affinity; LKB1 (1:1000, 10746-1-AP), p-LKB1 (1:5000, 80127-1-RR), AMPK (1:500, 10929-2-AP), p-AKT (1:5000, 66444-1-Ig), AKT (1:5000, 60203-2-Ig), GLUT4 (1:3000, 66846-1-Ig) from Proteintech; Bax (1:1000, GB12690) from Seville; Bcl-2 (1:1000, BA0412) and Nrf2 (1:1000, A0674) from BOSTER; Lamin-B1(1:1000, SI17-06) from Huabio.

Techniques:

Journal: PLOS ONE

Article Title: Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway

doi: 10.1371/journal.pone.0296792

Figure Lengend Snippet: (A) The cell viability of H9C2 cells was treated with HG with 0, 10, 30, and 50mM for 24 and 48 h. (B) The H9C2 cells were treated with IH and HG for 24 and 48 h, and cell viability of H9C2 cells was measured. (C) The cell viability of H9C2 cells treated with IH and HG. n = 6. (D-E) Representative images of mitochondrial membrane potential and quantitative analysis of the JC-1 ratio. n = 4. (F-G) Representative images of ROS and quantitative analysis of ROS. (H-K) Representative blot images and quantitative analysis of LKB1, AMPK, and Nrf2. n = 3. Data are presented as means ± S.E.M. * p < 0.05 and ** p < 0.01 vs. CON group; Φ p < 0.05 and ΦΦ p < 0.01 vs. HG group.

Article Snippet: Seal with 5% skim milk powder sealing solution for 2 h, adding primary antibody and incubating overnight at 4°C: Tubulin (1:10000, GTX628802) and Caspase-3(1:1000, #14220) from CST; p-AMPK (1:2000, AP0116) and HO-1(1:1000, A1346) from ABclonal; PI3K (1:1000, AF6241) from Affinity; LKB1 (1:1000, 10746-1-AP), p-LKB1 (1:5000, 80127-1-RR), AMPK (1:500, 10929-2-AP), p-AKT (1:5000, 66444-1-Ig), AKT (1:5000, 60203-2-Ig), GLUT4 (1:3000, 66846-1-Ig) from Proteintech; Bax (1:1000, GB12690) from Seville; Bcl-2 (1:1000, BA0412) and Nrf2 (1:1000, A0674) from BOSTER; Lamin-B1(1:1000, SI17-06) from Huabio.

Techniques: Membrane

Journal: PLOS ONE

Article Title: Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway

doi: 10.1371/journal.pone.0296792

Figure Lengend Snippet: (A-B) Representative images of mitochondrial membrane potential and quantitative analysis of the JC-1 ratio. (C-D) Representative images and quantitative analysis of ROS. (E-G) Representative blot images and quantitative analysis of AMPK and Nrf2. n = 3. Data are presented as means ± S.E.M. * p < 0.05 and ** p < 0.01 vs. CON group; Φ p < 0.05 and ΦΦ p < 0.01 vs. IH+HG group.

Article Snippet: Seal with 5% skim milk powder sealing solution for 2 h, adding primary antibody and incubating overnight at 4°C: Tubulin (1:10000, GTX628802) and Caspase-3(1:1000, #14220) from CST; p-AMPK (1:2000, AP0116) and HO-1(1:1000, A1346) from ABclonal; PI3K (1:1000, AF6241) from Affinity; LKB1 (1:1000, 10746-1-AP), p-LKB1 (1:5000, 80127-1-RR), AMPK (1:500, 10929-2-AP), p-AKT (1:5000, 66444-1-Ig), AKT (1:5000, 60203-2-Ig), GLUT4 (1:3000, 66846-1-Ig) from Proteintech; Bax (1:1000, GB12690) from Seville; Bcl-2 (1:1000, BA0412) and Nrf2 (1:1000, A0674) from BOSTER; Lamin-B1(1:1000, SI17-06) from Huabio.

Techniques: Membrane

Journal: Foods

Article Title: Response Surface Methodology Optimization of Exosome-like Nanovesicles Extraction from Lycium ruthenicum Murray and Their Inhibitory Effects on Aβ-Induced Apoptosis and Oxidative Stress in HT22 Cells

doi: 10.3390/foods13203328

Figure Lengend Snippet: Effects of LRM-ELNs on the Nrf2/HO-1/NQO1 signaling pathway in HT22 cells induced by Aβ. ( A – E ) The protein levels of nucl-Nrf2, cyto-Nrf2, HO-1, and NQO1 in HT22 cells were determined by WB. Data are expressed as mean ± SEM. ** p < 0.01 vs. the control group, ## p < 0.01 vs. the Aβ group.

Article Snippet: Antibodies for Nrf2 (A0674), HO-1 (A1346), NQO1 (A0047), Bax (A12009), Bcl2 (A19693), and Histone H3 (A17562) were purchased from ABclonal (Wuhan, China).

Techniques: Control

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Ficus pandurata Hance Inhibits Ulcerative Colitis and Colitis-Associated Secondary Liver Damage of Mice by Enhancing Antioxidation Activity

doi: 10.1155/2021/2617881

Figure Lengend Snippet: FPH enhances the colonic antioxidation in the UC mice induced by DSS. Effect of FPH on the levels of MDA (a), T-SOD (b), and GSH-Px (c) in the colonic homogenate detected by ELISA. (d) Effect of FPH on the protein expressions of Keap1, Nrf2, HO1, NQO1, p22-phox, and NOX2 in colon tissues detected by Western blot. Results were expressed as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01 vs. the control group; # p < 0.05, ## p < 0.01 vs. the model group (DSS only).

Article Snippet: Primary antibodies against Nrf2 (A0674) and NLRP3 (A5652) were obtained from ABclonal Biotechnology (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Control