Journal: BioMed Research International

Article Title: Ammonia Exposure Induced Cilia Dysfunction of Nasal Mucosa in the Piglets

doi: 10.1155/2020/1705387

Figure Lengend Snippet: Histopathological analysis of nasal mucosa. (a) Results of H&E staining of nasal mucosa. Bars = 50 μ m. (b) Results of AB-PAS staining of mucus produced by goblet cells and submucosal glands (arrow). Bars = 50 μ m. (c) Immunohistochemical staining of MUC5AC protein in the surface epithelium of nasal mucosa (arrow). Bars = 50 μ m. (d) Immunohistochemical staining of MUC5B protein in nasal mucosa (arrow). Bars = 50 μ m. (e) Statistical analysis of thickness of nasal mucosa. The pictures used for statistics have the same size, pixel value, shooting conditions, and magnification for each group, and 10 to 15 stained sections from each group were analyzed. (f) qPCR results of MUC5AC. (g) qPCR results of MUC5B. Data from (f) and (g) were analyzed with Student's t -test, and the values were presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01.

Article Snippet: The nose sections were fixed by 4% formaldehyde and then stained with anti-MUC5AC antibody (1 : 100, BA3293; Boster Corporation) and anti-MUC5B antibody (1 : 100, D261005; Sangon Biotech (Shanghai)) using the SABC IHC kits (SA1022/SA1028/SA1020, Boster Corporation (Wuhan)).

Techniques: Staining, Produced, Immunohistochemical staining

Journal: Cell Proliferation

Article Title: Characterization of porcine extraembryonic endoderm cells

doi: 10.1111/cpr.12591

Figure Lengend Snippet: Derivation and characterization of porcine extraembryonic endoderm cells (pXENCs). A, Formation of pXENCs (left) and of porcine‐induced pluripotent stem cells (piPSCs; right) during cultivation. B, Alkaline phosphatase (AP)‐negative pXENCs (left) and AP‐positive piPSCs (right). C, Quantitative real‐time PCR analysis for XEN cells markers ( Gata4 , Gata6 , Sox17 , Pdfgra , Hnf4a , Ihh , Apoe , Pth1r and Sparc ) in pXENCs and piPSCs. Data are represented as mean ± SD. * P < 0.05,** P <0.01 and *** P < 0.001. D, Immunofluorescence images showing the presence/absence (in green) of GATA6, GATA4, PDGFRα and SOX17 in pXENCs (left) and piPSCs (right). Scale bars: 100 µm (A), 50 µm (B), 200 µm (D)

Article Snippet: Primary antibodies were used against GATA4 (#BM5082; Boster Biological Technology, Wuhan, China) or against GATA6 (#AF1700), SOX17 (#AF1924) or PDGFRa (#AF1062; all from R&D Systems, Minneapolis, MN, USA).

Techniques: Real-time Polymerase Chain Reaction, Immunofluorescence

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Prognostic Value of β-Catenin and L1CAM Expressions in Type I Endometrial Carcinoma

doi: 10.31557/APJCP.2023.24.4.1413

Figure Lengend Snippet: A). Positive expression of β-catenin on the nucleus and cell cytoplasm of type I endometrial carcinoma Grade 2 (200x). B). Positive expression of L1CAM on cell membranes of type I endometrial carcinoma Grade 2 (200x)

Article Snippet: Immunohistochemical procedures were performed using β-catenin monoclonal antibodies (catalog No. GTX34442, dilution 1:50; Gene Tex Laboratory) and L1CAM polyclonal antibody (catalog No.A00729-1, dilution 1:50; Boster Biological Technology).

Techniques: Expressing

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Prognostic Value of β-Catenin and L1CAM Expressions in Type I Endometrial Carcinoma

doi: 10.31557/APJCP.2023.24.4.1413

Figure Lengend Snippet: A). β-catenin positive expression of tumor cells in the area of ​​Lymphovascular invasion (200x). B). L1CAM positive expression of tumor cells in the area of ​​lymphovascular invasion (100x). C). Positive expression of β-catenin in type I endometrial carcinoma that metastases to the omentum (100x). D). L1CAM positive expression in type I endometrial carcinoma metastases to the omentum (100x)

Article Snippet: Immunohistochemical procedures were performed using β-catenin monoclonal antibodies (catalog No. GTX34442, dilution 1:50; Gene Tex Laboratory) and L1CAM polyclonal antibody (catalog No.A00729-1, dilution 1:50; Boster Biological Technology).

Techniques: Expressing

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Prognostic Value of β-Catenin and L1CAM Expressions in Type I Endometrial Carcinoma

doi: 10.31557/APJCP.2023.24.4.1413

Figure Lengend Snippet: β-catenin and L1CAM Expressions in Type I Endometrial Carcinoma based on the Presence of Lymphovascular Space Invasion (LVSI)

Article Snippet: Immunohistochemical procedures were performed using β-catenin monoclonal antibodies (catalog No. GTX34442, dilution 1:50; Gene Tex Laboratory) and L1CAM polyclonal antibody (catalog No.A00729-1, dilution 1:50; Boster Biological Technology).

Techniques: Expressing

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Prognostic Value of β-Catenin and L1CAM Expressions in Type I Endometrial Carcinoma

doi: 10.31557/APJCP.2023.24.4.1413

Figure Lengend Snippet: β-catenin and L1CAM Expressions in Type I Endometrial Carcinoma based on the Presence of Lymph Node or Omental Metastases

Article Snippet: Immunohistochemical procedures were performed using β-catenin monoclonal antibodies (catalog No. GTX34442, dilution 1:50; Gene Tex Laboratory) and L1CAM polyclonal antibody (catalog No.A00729-1, dilution 1:50; Boster Biological Technology).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Effect of Mixed Transplantation of Autologous and Allogeneic Microskin Grafts on Wound Healing in a Rat Model of Acute Skin Defect

doi: 10.1371/journal.pone.0085672

Figure Lengend Snippet: Aa, Ab, and Ac show integrin β1 expression at 2 post-graft weeks (PGWs) in groups II,III, and IV, respectively, in Experiment A. Ad shows integrin β1 expression in the hair follicle in group IV in Experiment A. Ae and Af show integrin β1 expression at 2 PGWs in groups II and III, respectively, in Experiment B. Ag and Ah show expression of integrin β1 in the hair follicle and no expression of integrin β1 in the epidermis, respectively, of normal Sprague-Dawley (SD) rat skin. Ai, Aj, and Ak show negative control staining of 2 PGW sections from groups II, III, and IV, respectively, in Experiment A. Scale bar, 50 µm in Aa–Ah and 100 µm in Ai–Ak. In Experiment A, the mean integrated optical density (IOD) of integrin β1 expression at 2 PGWs was higher in groups III and IV than in group II (* p<0.05), and, more interestingly, was highest in group III among these 3 groups (* p<0.05, # p<0.05). At 3 and 4 PGWs, the integrin β1 expression remained higher in group III than in groups II and IV (* p<0.05, # p<0.05). In Experiment B, the integrin β1 expression at 2 PGWs was stronger in group III than in group I (* p<0.05), and no intergroup difference was found at 4 PGWs.

Article Snippet: The sections were then sequentially incubated with normal goat serum for 40 minutes to block nonspecific binding, with the primary rabbit anti-integrin β1 polyclonal antibody (1∶100, Wuhan Boster Biological Technology LTD, Hubei, China) for 15 hours at 4°C, and with the SABC kit (Wuhan Boster Biological Technology, LTD, Hubei, China) at 37°C to bind the primary antibody.

Techniques: Expressing, Negative Control, Staining